Q8PTR0 (MCH_METMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methenyltetrahydromethanopterin cyclohydrolase
EC=3.5.4.27

Alternative name(s):
Methenyl-H4MPT cyclohydrolase

Gene names

Name:	mch
Ordered Locus Names:	MM_2653

Organism

Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

192952 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible interconversion of 5-formyl-H4MPT to methenyl-H4MPT+ By similarity. HAMAP MF_00486
Catalytic activity	5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = 5-formyl-5,6,7,8-tetrahydromethanopterin. HAMAP MF_00486
Pathway	One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3. HAMAP MF_00486
Subcellular location	Cytoplasm By similarity HAMAP MF_00486.
Sequence similarities	Belongs to the MCH family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methenyltetrahydromethanopterin cyclohydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Methenyltetrahydromethanopterin cyclohydrolase HAMAP MF_00486		PRO_0000140883

Sequences

	Sequence		Length	Mass (Da)
	Q8PTR0 [UniParc]. Last modified October 10, 2002. Version 1. Checksum: E52D1A7D169FAA8E	FASTA	321	34,982
	10 20 30 40 50 60 MISVNEMGSY VIEEMLDWSE DLKTEVIKLE NGATIIDCGI KAEGGYEAGM YLARLCLADL 70 80 90 100 110 120 ADLKYSTFDL NGIKWPAIQV ATDNPVIACM ASQYAGWRIS VGNYFGMGSG PARALGLKPK 130 140 150 160 170 180 ELYEEIGYED DFEAAVLVME SDKLPDEKVV EYIAKHCSVD PENVMIAVAP TASIAGSVQI 190 200 210 220 230 240 SARVVETGIH KFESIGFDIN CIKSGYGIAP IAPIVGNDVQ CMGSTNDCVI YCGETNYTVS 250 260 270 280 290 300 FEGELAKLED FVRKVPSTTS DDFGKPFYQT FKAANFDFFK VDAGMFAPAR VTVNDLKNKK 310 320 TISSGGLYPE ILLESFGIRK V

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008384 Genomic DNA. Translation: AAM32349.1.
RefSeq	NP_634677.1. NC_003901.1.
3D structure databases
ProteinModelPortal	Q8PTR0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1480995.
GenomeReviews	Gene locus MM_2653 in contig AE008384_GR.
KEGG	mma:MM_2653.
NMPDR	fig|192952.1.peg.2653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497176.
OMA	YFAMGSG.
ProtClustDB	PRK02264.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM2653-MONOMER.
Family and domain databases
HAMAP	MF_00486. McH. [Tree]
InterPro	IPR003209. METHMP_CycHdrlase. [Graphical view]
KO	K01499.
Pfam	PF02289. MCH. 1 hit. [Graphical view]
SUPFAM	SSF56199. Cyclohydrolase. 1 hit.
TIGRFAMs	TIGR03120. One_C_mch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCH_METMA

Accession

Primary (citable) accession number: Q8PTR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 10, 2002
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program