Reviewed, UniProtKB/Swiss-Prot Q8PTR0 (MCH_METMA)

Names and origin

Protein names

Recommended name:
    Methenyltetrahydromethanopterin cyclohydrolase
    EC=3.5.4.27
Alternative name(s):
    Methenyl-H4MPT cyclohydrolase

Gene names

Name:	mch
Ordered Locus Names:	MM_2653

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible interconversion of 5-formyl-H4MPT to methenyl-H4MPT+ By similarity.
Catalytic activity	5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = 5-formyl-5,6,7,8-tetrahydromethanopterin. HAMAP MF_00486
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 3/3. HAMAP MF_00486
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MCH family.

Ontologies

Keywords
Biological process	Methanogenesis One-carbon metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methenyltetrahydromethanopterin cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Methenyltetrahydromethanopterin cyclohydrolase HAMAP MF_00486		PRO_0000140883

Sequences

	Sequence		Length	Mass (Da)
	Q8PTR0-1 [UniParc]. Last modified October 10, 2002. Version 1. Checksum: E52D1A7D169FAA8E	FASTA	321	34,982
	10 20 30 40 50 60 MISVNEMGSY VIEEMLDWSE DLKTEVIKLE NGATIIDCGI KAEGGYEAGM YLARLCLADL 70 80 90 100 110 120 ADLKYSTFDL NGIKWPAIQV ATDNPVIACM ASQYAGWRIS VGNYFGMGSG PARALGLKPK 130 140 150 160 170 180 ELYEEIGYED DFEAAVLVME SDKLPDEKVV EYIAKHCSVD PENVMIAVAP TASIAGSVQI 190 200 210 220 230 240 SARVVETGIH KFESIGFDIN CIKSGYGIAP IAPIVGNDVQ CMGSTNDCVI YCGETNYTVS 250 260 270 280 290 300 FEGELAKLED FVRKVPSTTS DDFGKPFYQT FKAANFDFFK VDAGMFAPAR VTVNDLKNKK 310 320 TISSGGLYPE ILLESFGIRK V

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM32349.1.
RefSeq	NP_634677.1.
3D structure databases
HSSP	HSSP built from PDB template 1QLM based on UniProtKB P94954.
ModBase	Search...
Genome annotation databases
GeneID	1480995.
GenomeReviews	Gene locus MM_2653 in contig AE008384_GR.
KEGG	mma:MM_2653.
NMPDR	fig|192952.1.peg.2653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PTR0.
OMA	Q8PTR0. YFAMGSG.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM2653-MON.
BRENDA	3.5.4.27. 261165.
Family and domain databases
HAMAP	MF_00486. [Tree]
InterPro	IPR003209. METHMP_CycHdrlase. [Graphical view]
Pfam	PF02289. MCH. 1 hit. [Graphical view]
ProDom	PD011637. Cyclohydrolase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03120. one_C_mch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCH_METMA

Accession

Primary (citable) accession number: Q8PTR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	October 10, 2002
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)