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Q8PTK1 (DNLI2_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 2

EC=6.5.1.1
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] 2
Gene names
Name:lig2
Ordered Locus Names:MM_2714
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 568568DNA ligase 2 HAMAP-Rule MF_00407
PRO_0000059606

Sites

Active site2561N6-AMP-lysine intermediate By similarity
Binding site2541ATP By similarity
Binding site2611ATP By similarity
Binding site2761ATP By similarity
Binding site3061ATP By similarity
Binding site3461ATP By similarity
Binding site4251ATP By similarity
Binding site4311ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8PTK1 [UniParc].

Last modified October 10, 2002. Version 1.
Checksum: D98B7C62AD2B6751

FASTA56863,185
        10         20         30         40         50         60 
MTSFREFAET CQAIEKISST IETTNKVADL LKKVDVEELP VATHFIMSEV FPAWSGEQLG 

        70         80         90        100        110        120 
IGTSLLYVSL SKASGMSIHS IESLVRTTGD IGDTALLILK EKRKNQVTFS SFFEEKPELS 

       130        140        150        160        170        180 
ITEVYRRFKI ASEASGKGSQ DIKVKNLQFL FTSSSPREAK YISRLALEEL RIGVGEGVVR 

       190        200        210        220        230        240 
DAIAKAFSVP AEIVEHSFMV TNDLGIVAAA AKKGGVEALE RLGIEINRPI KMMLSQISPD 

       250        260        270        280        290        300 
IDADIRAMKE VAIEWKFDGA RVQIHKDGNS VTLFSRKLEN VTSSLPDLVE IVRKHVKAES 

       310        320        330        340        350        360 
AILDGEAVAV DENGVPRAFQ EILKRFRRKY DVREKALGIP IQLNFFDIMY INGKTLIDLP 

       370        380        390        400        410        420 
LLERRKALES CVESSVEDSK SISVAEQVIT GDLELVEKIY REALKAGHEG VMVKNPNSVY 

       430        440        450        460        470        480 
SPGKRGKNWL KKKPLMDTLD LVIVGAEWGF GRRANLIGSY TVACYDPDTT RFLQVGKVGT 

       490        500        510        520        530        540 
GLTDDQLKEL TEILSGLMEG GEAGGVFAIR PKVVLEIAFE EIQKSPNYDS GFALRFPRFI 

       550        560 
RIRDDKDPEE ADTIQRIGRV YSQQLKRL 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM32410.1.
RefSeqNP_634738.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PTK1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_2714.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM32410; AAM32410; MM_2714.
GeneID1481056.
KEGGmma:MM_2714.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000036008.
KOK10747.
OMAGRPRPFQ.
ProtClustDBCLSK568925.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-2775-MONOMER.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SUPFAMSSF117018. SSF117018. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI2_METMA
AccessionPrimary (citable) accession number: Q8PTK1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: February 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families