Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8PTK1

- DNLI2_METMA

UniProt

Q8PTK1 - DNLI2_METMA

Protein

DNA ligase 2

Gene

lig2

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (10 Oct 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.UniRule annotation

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).UniRule annotation

    Cofactori

    Divalent metal cations.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei254 – 2541ATPUniRule annotation
    Active sitei256 – 2561N6-AMP-lysine intermediateUniRule annotation
    Binding sitei261 – 2611ATPUniRule annotation
    Binding sitei276 – 2761ATPUniRule annotation
    Binding sitei306 – 3061ATPUniRule annotation
    Binding sitei346 – 3461ATPUniRule annotation
    Binding sitei425 – 4251ATPUniRule annotation
    Binding sitei431 – 4311ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: UniProtKB-HAMAP
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. DNA ligation involved in DNA repair Source: InterPro
    4. DNA recombination Source: UniProtKB-HAMAP
    5. DNA replication Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell cycle, Cell division, DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMMAZ192952:GCK2-2775-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase 2UniRule annotation (EC:6.5.1.1UniRule annotation)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP] 2UniRule annotation
    Gene namesi
    Name:lig2UniRule annotation
    Ordered Locus Names:MM_2714
    OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
    Taxonomic identifieri192952 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
    ProteomesiUP000000595: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 568568DNA ligase 2PRO_0000059606Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi192952.MM_2714.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8PTK1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1793.
    HOGENOMiHOG000036008.
    KOiK10747.
    OMAiARVQVHK.

    Family and domain databases

    Gene3Di1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPiMF_00407. DNA_ligase.
    InterProiIPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8PTK1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSFREFAET CQAIEKISST IETTNKVADL LKKVDVEELP VATHFIMSEV    50
    FPAWSGEQLG IGTSLLYVSL SKASGMSIHS IESLVRTTGD IGDTALLILK 100
    EKRKNQVTFS SFFEEKPELS ITEVYRRFKI ASEASGKGSQ DIKVKNLQFL 150
    FTSSSPREAK YISRLALEEL RIGVGEGVVR DAIAKAFSVP AEIVEHSFMV 200
    TNDLGIVAAA AKKGGVEALE RLGIEINRPI KMMLSQISPD IDADIRAMKE 250
    VAIEWKFDGA RVQIHKDGNS VTLFSRKLEN VTSSLPDLVE IVRKHVKAES 300
    AILDGEAVAV DENGVPRAFQ EILKRFRRKY DVREKALGIP IQLNFFDIMY 350
    INGKTLIDLP LLERRKALES CVESSVEDSK SISVAEQVIT GDLELVEKIY 400
    REALKAGHEG VMVKNPNSVY SPGKRGKNWL KKKPLMDTLD LVIVGAEWGF 450
    GRRANLIGSY TVACYDPDTT RFLQVGKVGT GLTDDQLKEL TEILSGLMEG 500
    GEAGGVFAIR PKVVLEIAFE EIQKSPNYDS GFALRFPRFI RIRDDKDPEE 550
    ADTIQRIGRV YSQQLKRL 568
    Length:568
    Mass (Da):63,185
    Last modified:October 10, 2002 - v1
    Checksum:iD98B7C62AD2B6751
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008384 Genomic DNA. Translation: AAM32410.1.
    RefSeqiNP_634738.1. NC_003901.1.

    Genome annotation databases

    EnsemblBacteriaiAAM32410; AAM32410; MM_2714.
    GeneIDi1481056.
    KEGGimma:MM_2714.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE008384 Genomic DNA. Translation: AAM32410.1 .
    RefSeqi NP_634738.1. NC_003901.1.

    3D structure databases

    ProteinModelPortali Q8PTK1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 192952.MM_2714.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAM32410 ; AAM32410 ; MM_2714 .
    GeneIDi 1481056.
    KEGGi mma:MM_2714.

    Phylogenomic databases

    eggNOGi COG1793.
    HOGENOMi HOG000036008.
    KOi K10747.
    OMAi ARVQVHK.

    Enzyme and pathway databases

    BioCyci MMAZ192952:GCK2-2775-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3260.10. 1 hit.
    2.40.50.140. 1 hit.
    HAMAPi MF_00407. DNA_ligase.
    InterProi IPR022865. DNA_ligae_ATP-dep_bac/arc.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117018. SSF117018. 1 hit.
    SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
      Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
      , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
      J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

    Entry informationi

    Entry nameiDNLI2_METMA
    AccessioniPrimary (citable) accession number: Q8PTK1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2002
    Last sequence update: October 10, 2002
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3