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Q8PSV9

- SYI_METMA

UniProt

Q8PSV9 - SYI_METMA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-3030-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MM_2967
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000000595: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10581058Isoleucine--tRNA ligasePRO_0000098584Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_2967.

Structurei

3D structure databases

ProteinModelPortaliQ8PSV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi596 – 6005"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PSV9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIKEITAKYD AEQIEKKVTQ FWEDSDAYRK TREHRKSGKR LFFVDGPPYT
60 70 80 90 100
TGHIHLGTAW NKIIKDSILR YYSMNNRYIL ERPGWDMHGL PIEVKVEGVL
110 120 130 140 150
GFKSKKDIES FGVENFIEKC KEFAIKQKQA MTEQFQRLGV WLQWPDPYMT
160 170 180 190 200
LKDEYIEAAW WTLKQASEKD LLEVGKRSVN WCPRCETAIA DSEVEYSERT
210 220 230 240 250
DPSIYVKFRV KGEENTFIVI WTTTPWTIPA NVAVAVHPAY EYSKFRAIRQ
260 270 280 290 300
DGSEEILIAA TELIKNVLKQ GRYTDFEVLE TMLGEELTKL EYESPVGDLV
310 320 330 340 350
PVQNEIKHGV YLADFVTVEN TGCVHIAPGH GMDDFNLGAK HKLPILCPVG
360 370 380 390 400
SNGSYTEEAG EYAGKNVKEA NPIVIEDLKA RNRLLAEGTV THRYGHCWRC
410 420 430 440 450
KTPIIYLATE QWFLKVTEIK EKMLEEIDAV DWYPDWAGSA RFRTWVEGAR
460 470 480 490 500
DWCISRQRYW GIPIPVWKCK KCGKLEVIGT KAELLEKAGL NGDIELHRPY
510 520 530 540 550
VDRVTVPCEC GGEKKRVEDV FDVWFDSAVA SWATLKFPQT RDQFDEWWPA
560 570 580 590 600
DFITEGHDQT RGWFYSQLGA SMVGFGRAPY KSVLMHGFTL DAGGKKMSKS
610 620 630 640 650
LGNVISPLDI IGRFGADTLR AYVLSSSAPW DDLKFNLEEV ETIHRSINIL
660 670 680 690 700
WNVFRFPLPY MALDNFDPMQ VSLDSVRDAL REEDRWILSR AQSVVKSVDE
710 720 730 740 750
AMSGYLLHKA VREILDFTLE DLSRWYIQLI RPRTWTEADD PDKLAAYCVL
760 770 780 790 800
YEVYVTITKL ISPFMPYLAE EMYQNLIRNV DPKAPESVHM CDWPKVNEAY
810 820 830 840 850
LDTKLEEAMN TARSIVEAAS NARQKAGRKL RWPISRIVVS PESEDAARAV
860 870 880 890 900
ERLRSVLMDQ TNSKDIVLTG VGKSWDELGL EVIPDPGKIG PVFKRDAGKV
910 920 930 940 950
VPALQKVDGF ALKKAFAEAG EFELTLADGS TVKVTPEMAN FKETLPEGTA
960 970 980 990 1000
SAESDAGPVY VDANLTPELE AEGYAREVIR RLQDMRKELD LVVDENIQVS
1010 1020 1030 1040 1050
VRIEDERVLK LVETLKGLIA EEVRADVFDI GSGVNVSGDL VKDWDVEGIA

MKMGIAKK
Length:1,058
Mass (Da):120,411
Last modified:October 1, 2002 - v1
Checksum:i8BA5C0DE0FDCE4F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008384 Genomic DNA. Translation: AAM32663.1.
RefSeqiNP_634991.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM32663; AAM32663; MM_2967.
GeneIDi1481309.
KEGGimma:MM_2967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008384 Genomic DNA. Translation: AAM32663.1 .
RefSeqi NP_634991.1. NC_003901.1.

3D structure databases

ProteinModelPortali Q8PSV9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 192952.MM_2967.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM32663 ; AAM32663 ; MM_2967 .
GeneIDi 1481309.
KEGGi mma:MM_2967.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MMAZ192952:GCK2-3030-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiSYI_METMA
AccessioniPrimary (citable) accession number: Q8PSV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3