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Q8PSV9

- SYI_METMA

UniProt

Q8PSV9 - SYI_METMA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, MM_2967
Organism
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei599 – 5991ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMMAZ192952:GCK2-3030-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:MM_2967
OrganismiMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia)
Taxonomic identifieri192952 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
ProteomesiUP000000595: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10581058Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098584Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi192952.MM_2967.

Structurei

3D structure databases

ProteinModelPortaliQ8PSV9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi596 – 6005"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PSV9-1 [UniParc]FASTAAdd to Basket

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MIKEITAKYD AEQIEKKVTQ FWEDSDAYRK TREHRKSGKR LFFVDGPPYT     50
TGHIHLGTAW NKIIKDSILR YYSMNNRYIL ERPGWDMHGL PIEVKVEGVL 100
GFKSKKDIES FGVENFIEKC KEFAIKQKQA MTEQFQRLGV WLQWPDPYMT 150
LKDEYIEAAW WTLKQASEKD LLEVGKRSVN WCPRCETAIA DSEVEYSERT 200
DPSIYVKFRV KGEENTFIVI WTTTPWTIPA NVAVAVHPAY EYSKFRAIRQ 250
DGSEEILIAA TELIKNVLKQ GRYTDFEVLE TMLGEELTKL EYESPVGDLV 300
PVQNEIKHGV YLADFVTVEN TGCVHIAPGH GMDDFNLGAK HKLPILCPVG 350
SNGSYTEEAG EYAGKNVKEA NPIVIEDLKA RNRLLAEGTV THRYGHCWRC 400
KTPIIYLATE QWFLKVTEIK EKMLEEIDAV DWYPDWAGSA RFRTWVEGAR 450
DWCISRQRYW GIPIPVWKCK KCGKLEVIGT KAELLEKAGL NGDIELHRPY 500
VDRVTVPCEC GGEKKRVEDV FDVWFDSAVA SWATLKFPQT RDQFDEWWPA 550
DFITEGHDQT RGWFYSQLGA SMVGFGRAPY KSVLMHGFTL DAGGKKMSKS 600
LGNVISPLDI IGRFGADTLR AYVLSSSAPW DDLKFNLEEV ETIHRSINIL 650
WNVFRFPLPY MALDNFDPMQ VSLDSVRDAL REEDRWILSR AQSVVKSVDE 700
AMSGYLLHKA VREILDFTLE DLSRWYIQLI RPRTWTEADD PDKLAAYCVL 750
YEVYVTITKL ISPFMPYLAE EMYQNLIRNV DPKAPESVHM CDWPKVNEAY 800
LDTKLEEAMN TARSIVEAAS NARQKAGRKL RWPISRIVVS PESEDAARAV 850
ERLRSVLMDQ TNSKDIVLTG VGKSWDELGL EVIPDPGKIG PVFKRDAGKV 900
VPALQKVDGF ALKKAFAEAG EFELTLADGS TVKVTPEMAN FKETLPEGTA 950
SAESDAGPVY VDANLTPELE AEGYAREVIR RLQDMRKELD LVVDENIQVS 1000
VRIEDERVLK LVETLKGLIA EEVRADVFDI GSGVNVSGDL VKDWDVEGIA 1050
MKMGIAKK 1058
Length:1,058
Mass (Da):120,411
Last modified:October 1, 2002 - v1
Checksum:i8BA5C0DE0FDCE4F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008384 Genomic DNA. Translation: AAM32663.1.
RefSeqiNP_634991.1. NC_003901.1.
WP_011034868.1. NC_003901.1.

Genome annotation databases

EnsemblBacteriaiAAM32663; AAM32663; MM_2967.
GeneIDi1481309.
KEGGimma:MM_2967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008384 Genomic DNA. Translation: AAM32663.1 .
RefSeqi NP_634991.1. NC_003901.1.
WP_011034868.1. NC_003901.1.

3D structure databases

ProteinModelPortali Q8PSV9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 192952.MM_2967.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM32663 ; AAM32663 ; MM_2967 .
GeneIDi 1481309.
KEGGi mma:MM_2967.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MMAZ192952:GCK2-3030-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
    Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R.
    , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
    J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Entry informationi

Entry nameiSYI_METMA
AccessioniPrimary (citable) accession number: Q8PSV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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