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Q8PS70 (CAPPA_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase

Short name=PEPC
Short name=PEPCase
EC=4.1.1.31
Gene names
Name:ppcA
Ordered Locus Names:MM_3212
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length526 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the irreversible beta-carboxylation of phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle By similarity. HAMAP-Rule MF_01904

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_01904

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01904

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01904

Sequence similarities

Belongs to the PEPCase type 2 family.

Ontologies

Keywords
   Biological processCarbon dioxide fixation
   LigandMagnesium
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbon fixation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

oxaloacetate metabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: InterPro

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

phosphoenolpyruvate carboxylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 526526Phosphoenolpyruvate carboxylase HAMAP-Rule MF_01904
PRO_0000309604

Sequences

Sequence LengthMass (Da)Tools
Q8PS70 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 7CCCE37156EF8B55

FASTA52658,177
        10         20         30         40         50         60 
MSKKATYPKV MCTQHPDSAS KYISTQEEPG EAIEAAVVFG CDEYMPDYEG KATPYHQNVQ 

        70         80         90        100        110        120 
IVSRFIEETD LIPGKDIFIT PRAPSAAQEN RFRQLMVMMS IAEANHGALE YSDVQAINEF 

       130        140        150        160        170        180 
VHPMTGTVGE ILDAQQHMVD VGELAKKEFG VAMEVPRIIP LIEDAPALLH AKELAENTLL 

       190        200        210        220        230        240 
SWEKRFGAAP EKFRVFLGKS DSALSFGHVA STLSCKYAIS GISELDLELD TSTGIIFGAG 

       250        260        270        280        290        300 
TLPFRGHLSL KNAENFFREY RGIGTITLQS AVRYSHDKGE AEALVRLAEE RLPESPEIYS 

       310        320        330        340        350        360 
GEEKEEIVNL IGIFGARYNR IIREMSCTIN QLAGLLPQQR DRLMHKGTGG YSRNVPDISG 

       370        380        390        400        410        420 
LVCLCRKDVG KELSASMPAE DLNLPRAIKF TGALYSIGLP PEFIGTGTAL EEAREKLGEE 

       430        440        450        460        470        480 
ACERLLKKYF PSLASDLSFA SGYLDLNVAS RFLPEACLKE IRKDIEVLRD TFSLKVQPEP 

       490        500        510        520 
SYRILLEMMQ PDLLQAGTKG NCMDEEVSQL VCSTLTKMGK IRKALG 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM32908.1.
RefSeqNP_635236.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PS70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_3212.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM32908; AAM32908; MM_3212.
GeneID1481554.
KEGGmma:MM_3212.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1892.
HOGENOMHOG000009826.
KOK01595.
OMAPRAIKFT.
ProtClustDBPRK13655.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-3277-MONOMER.

Family and domain databases

HAMAPMF_01904. PEPcase_type2.
InterProIPR007566. PEP_COase_arc-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF14010. PEPcase_2. 1 hit.
[Graphical view]
PIRSFPIRSF006677. UCP006677. 1 hit.
SUPFAMSSF51621. SSF51621. 1 hit.
TIGRFAMsTIGR02751. PEPCase_arch. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCAPPA_METMA
AccessionPrimary (citable) accession number: Q8PS70
Entry history
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: October 1, 2002
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families