ID   THIM_METMA              Reviewed;         261 AA.
AC   Q8PS50;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 51.
DE   RecName: Full=Hydroxyethylthiazole kinase;
DE            EC=2.7.1.50;
DE   AltName: Full=4-methyl-5-beta-hydroxyethylthiazole kinase;
DE            Short=Thz kinase;
DE            Short=TH kinase;
GN   Name=thiM; OrderedLocusNames=MM_3234;
OS   Methanosarcina mazei (Methanosarcina frisia).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales;
OC   Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=2209;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88;
RX   MEDLINE=22120827; PubMed=12125824;
RA   Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A.,
RA   Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C.,
RA   Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S.,
RA   Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P.,
RA   Fritz H.-J., Gottschalk G.;
RT   "The genome of Methanosarcina mazei: evidence for lateral gene
RT   transfer between Bacteria and Archaea.";
RL   J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-methyl-5-(2-hydroxyethyl)thiazole =
CC       ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.
CC   -!- COFACTOR: Binds 2 magnesium ions per subunit (By similarity).
CC   -!- PATHWAY: Cofactor biosynthesis; thiamine pyrophosphate
CC       biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-
CC       5-(2-hydroxyethyl)-thiazole: step 1/1.
CC   -!- SIMILARITY: Belongs to the Thz kinase family.
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DR   EMBL; AE008384; AAM32930.1; -; Genomic_DNA.
DR   RefSeq; NP_635258.1; -.
DR   HSSP; P39593; 1EKQ.
DR   GeneID; 1481576; -.
DR   GenomeReviews; AE008384_GR; MM_3234.
DR   KEGG; mma:MM_3234; -.
DR   NMPDR; fig|192952.1.peg.3234; -.
DR   HOGENOM; Q8PS50; -.
DR   OMA; Q8PS50; ASPVMAH.
DR   BioCyc; MMAZ192952:MM3234-MON; -.
DR   BRENDA; 2.7.1.50; 261165.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004417; F:hydroxyethylthiazole kinase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009228; P:thiamin biosynthetic process; IEA:UniProtKB-KW.
DR   HAMAP; MF_00228; -; 1.
DR   InterPro; IPR000417; Hyethyz_kinase.
DR   InterPro; IPR011144; Hyethyz_kinsmonf.
DR   PANTHER; PTHR20857:SF14; Hyethyz_kinase; 1.
DR   Pfam; PF02110; HK; 1.
DR   PIRSF; PIRSF000513; Thz_kinase; 1.
DR   PRINTS; PR01099; HYETHTZKNASE.
DR   TIGRFAMs; TIGR00694; thiM; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Kinase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Thiamine biosynthesis; Transferase.
FT   CHAIN         1    261       Hydroxyethylthiazole kinase.
FT                                /FTId=PRO_0000156969.
FT   METAL        89     89       Magnesium 1 (By similarity).
FT   METAL       121    121       Magnesium 1 (By similarity).
FT   BINDING      40     40       Substrate; via amide nitrogen (By
FT                                similarity).
FT   BINDING     116    116       ATP (By similarity).
FT   BINDING     162    162       ATP (By similarity).
FT   BINDING     189    189       Substrate; via amide nitrogen (By
FT                                similarity).
SQ   SEQUENCE   261 AA;  27555 MW;  5D5128BAEECBC3EC CRC64;
     MKNPLKTIRE TKPLVHHITN WVTIYDCANI TRAFGALPVM AHAPEECADM TGISSALVLN
     IGTLTSEIID SMLLSAAAAN KKKIPVVLDA VGVGATRFRD EMAAKILGSV RVDIIKGNYS
     EIAKLAGENA ETKGVESTSI SADPQKVAKE FAKSSSSVVV MTGKEDVISD GNRTFVVKNG
     HELMGSIVGT GCMAASIIGS FASVNPDYCD AAKDALCYFG AAGELAAKNS AGPGSFKVNL
     YDEVFNLSDE KVQAMMKVEE L
//