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Q8PS50 (THIM_METMA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:MM_3234
OrganismMethanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) (Methanosarcina frisia) [Complete proteome] [HAMAP]
Taxonomic identifier192952 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000156969

Sites

Binding site401Substrate; via amide nitrogen By similarity
Binding site1161ATP By similarity
Binding site1621ATP By similarity
Binding site1891Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8PS50 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 5D5128BAEECBC3EC

FASTA26127,555
        10         20         30         40         50         60 
MKNPLKTIRE TKPLVHHITN WVTIYDCANI TRAFGALPVM AHAPEECADM TGISSALVLN 

        70         80         90        100        110        120 
IGTLTSEIID SMLLSAAAAN KKKIPVVLDA VGVGATRFRD EMAAKILGSV RVDIIKGNYS 

       130        140        150        160        170        180 
EIAKLAGENA ETKGVESTSI SADPQKVAKE FAKSSSSVVV MTGKEDVISD GNRTFVVKNG 

       190        200        210        220        230        240 
HELMGSIVGT GCMAASIIGS FASVNPDYCD AAKDALCYFG AAGELAAKNS AGPGSFKVNL 

       250        260 
YDEVFNLSDE KVQAMMKVEE L 

« Hide

References

[1]"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea."
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. expand/collapse author list , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.
J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008384 Genomic DNA. Translation: AAM32930.1.
RefSeqNP_635258.1. NC_003901.1.

3D structure databases

ProteinModelPortalQ8PS50.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING192952.MM_3234.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM32930; AAM32930; MM_3234.
GeneID1481576.
KEGGmma:MM_3234.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OMAQTFTANV.

Enzyme and pathway databases

BioCycMMAZ192952:GCK2-3299-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_METMA
AccessionPrimary (citable) accession number: Q8PS50
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways