Reviewed, UniProtKB/Swiss-Prot Q8PS50 (THIM_METMA)

Names and origin

Protein names

Recommended name:
    Hydroxyethylthiazole kinase
    EC=2.7.1.50
Alternative name(s):
    4-methyl-5-beta-hydroxyethylthiazole kinase
      Short name=Thz kinase
      Short name=TH kinase

Gene names

Name:	thiM
Ordered Locus Names:	MM_3234

Organism

Methanosarcina mazei (Methanosarcina frisia) [Complete proteome] [HAMAP]

Taxonomic identifier

2209 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP MF_00228
Cofactor	Binds 2 magnesium ions per subunit By similarity.
Pathway	Cofactor biosynthesis; thiamine pyrophosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 4-methyl-5-(2-hydroxyethyl)-thiazole: step 1/1. HAMAP MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	thiamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Hydroxyethylthiazole kinase HAMAP MF_00228		PRO_0000156969
Sites
	Metal binding	89	1	Magnesium 1 By similarity
	Metal binding	121	1	Magnesium 1 By similarity
	Binding site	40	1	Substrate; via amide nitrogen By similarity
	Binding site	116	1	ATP By similarity
	Binding site	162	1	ATP By similarity
	Binding site	189	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PS50-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 5D5128BAEECBC3EC	FASTA	261	27,555
	10 20 30 40 50 60 MKNPLKTIRE TKPLVHHITN WVTIYDCANI TRAFGALPVM AHAPEECADM TGISSALVLN 70 80 90 100 110 120 IGTLTSEIID SMLLSAAAAN KKKIPVVLDA VGVGATRFRD EMAAKILGSV RVDIIKGNYS 130 140 150 160 170 180 EIAKLAGENA ETKGVESTSI SADPQKVAKE FAKSSSSVVV MTGKEDVISD GNRTFVVKNG 190 200 210 220 230 240 HELMGSIVGT GCMAASIIGS FASVNPDYCD AAKDALCYFG AAGELAAKNS AGPGSFKVNL 250 260 YDEVFNLSDE KVQAMMKVEE L

References

[1]

"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea." Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R. , Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G. J. Mol. Microbiol. Biotechnol. 4:453-461(2002) [PubMed: 12125824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008384 Genomic DNA. Translation: AAM32930.1.
RefSeq	NP_635258.1.
3D structure databases
HSSP	HSSP built from PDB template 1EKQ based on UniProtKB P39593.
ModBase	Search...
Genome annotation databases
GeneID	1481576.
GenomeReviews	Gene locus MM_3234 in contig AE008384_GR.
KEGG	mma:MM_3234.
NMPDR	fig|192952.1.peg.3234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PS50.
OMA	Q8PS50. ASPVMAH.
Enzyme and pathway databases
BioCyc	MMAZ192952:MM3234-MON.
BRENDA	2.7.1.50. 261165.
Family and domain databases
HAMAP	MF_00228. [Tree]
InterPro	IPR000417. Hyethyz_kinase. IPR011144. Hyethyz_kinsmonf. [Graphical view]
PANTHER	PTHR20857:SF14. Hyethyz_kinase. 1 hit.
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_METMA

Accession

Primary (citable) accession number: Q8PS50

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)