Reviewed, UniProtKB/Swiss-Prot Q8PR33 (LLDD_XANAC)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase [cytochrome]
    EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	XAC0133

Organism

Xanthomonas axonopodis pv. citri (Citrus canker) [Complete proteome] [HAMAP]

Taxonomic identifier

92829 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP MF_01559
Cofactor	FMN By similarity.
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	L-lactate dehydrogenase [cytochrome] HAMAP MF_01559		PRO_0000206354
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8PR33-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: E60891DE3F3D5FC3	FASTA	388	41,830
	10 20 30 40 50 60 MIISAASDYR AAAEARLPPF LFHYIDGGAY AEHTLRRNVS DLADVALRQR VLRNMSDLRL 70 80 90 100 110 120 STELFGETLA MPVALAPVGL TGMYARRGEV QAARAAAARG IPFTLSTVSV CPIEEVAPAI 130 140 150 160 170 180 ERPMWFQLYV LKDRGFMRNA LERAKAAGVT TLVFTVDMPT PGARYRDAHS GMSGPNASLR 190 200 210 220 230 240 RMLQAVTHPR WAWDVGLLGK PHDLGNISAY RGSPTGLQDY IGWLGANFDP SIAWKDLEWI 250 260 270 280 290 300 REFWTGPMVI KGILDPEDAR DAVRFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGE 310 320 330 340 350 360 LKILADSGIR SGLDVVRMLA LGADAVLLGR AFVYALAAAG QAGVENLLTL IEKEMRVAMT 370 380 LTGTHSIADI SADALSRVTR ERADAISP

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 306.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008923 Genomic DNA. Translation: AAM35025.1.
RefSeq	NP_640489.1.
3D structure databases
HSSP	HSSP built from PDB template 1GOX based on UniProtKB P05414.
ModBase	Search...
Genome annotation databases
GeneID	1154204.
GenomeReviews	Gene locus XAC0133 in contig AE008923_GR.
KEGG	xac:XAC0133.
NMPDR	fig|190486.1.peg.133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PR33.
OMA	ETRLFDE.
Enzyme and pathway databases
BioCyc	XAXO190486:XAC0133-MON.
BRENDA	1.1.2.3. 289771.
Family and domain databases
HAMAP	MF_01559. [Tree]
InterPro	IPR012133. a-Hydoxy_acid_DH_FMN. IPR013785. Aldolase_TIM. IPR000262. FMN-dep_DH. IPR017934. FMN-dep_OHA_DH. IPR008259. FMN_hydac_DH_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_XANAC

Accession

Primary (citable) accession number: Q8PR33

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)