Reviewed, UniProtKB/Swiss-Prot Q8PQA2 (GLGB2_XANAC)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 2
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 2
      Short name=BE 2
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2

Gene names

Name:	glgB2
Ordered Locus Names:	XAC0426

Organism

Xanthomonas axonopodis pv. citri (Citrus canker) [Complete proteome] [HAMAP]

Taxonomic identifier

92829 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 719	719	1,4-alpha-glucan-branching enzyme 2 HAMAP MF_00685		PRO_0000188765
Sites
	Active site	293	1	By similarity
	Active site	328	1	By similarity
	Active site	333	1	By similarity
	Active site	396	1	By similarity
	Active site	398	1	By similarity
	Active site	451	1	By similarity
	Active site	518	1	By similarity
	Active site	519	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PQA2-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 9E992FD6268E8E02	FASTA	719	79,256
	10 20 30 40 50 60 MSQTLQALAD GLPADAFAVL GPKLLADGRR QVRVLAPGAE AMGLIDPRGK LLARMQASAI 70 80 90 100 110 120 DGVFEGTLAV DGPYRLRIVW PDRVQEVEDP YAFAATLDES LLLQIAAGDG QALRRALGAQ 130 140 150 160 170 180 HLHCGDVPGV RFALWAPHAQ RVAVVGDFNG WDVRRHPMRQ RIGGFWELFL PRVDAGARYK 190 200 210 220 230 240 YAVTAADGRV LLKADPVARQ TELPPATASV VPSAATFAWT DAAWMANRDP AAVPGPLSIY 250 260 270 280 290 300 EVHAASWRRD GHNQPLDWPT LAEQLIPYVQ QLGFTHIELL PITEHPFGGS WGYQPLGLYA 310 320 330 340 350 360 PTARHGSPDG FAQFVDACHR AGIGVILDWV SAHFPDDAHG LAQFDGAALY EHADPREGMH 370 380 390 400 410 420 RDWNTLIYNY GRPEVTAYLL GSALEWIEHY HLDGLRVDAV ASMLYRDYGR AEGEWVPNAH 430 440 450 460 470 480 GGRENLEAVA FLRQLNRDIA TQFPGVLTIA EESTAWPGVT AAISDGGLGF THKWNMGWMH 490 500 510 520 530 540 DTLGYMQRDP AERAHHHSQL TFGLVYAFDE RFVLPLSHDE VVHGTGGLLG QMPGDDWRRF 550 560 570 580 590 600 ANLRAYLALM WAHPGDKLLF MGAEFGQWAD WNHDQSLDWH LLDGARHRGM QQLVGDLNAA 610 620 630 640 650 660 LRRTPALYRG THRAEGFDWS VADDARNSVL AFVRHDPDGG APLLAVSNLT PQPHHDYHVG 670 680 690 700 710 VPRAGLWREI LNTDSAHYGG SNLGNSGRLA TEPVGMHGHA QRLRLTLPPL ATIYLQAEK

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 306.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008923 Genomic DNA. Translation: AAM35317.1.
RefSeq	NP_640781.2.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q8PQA2.
Genome annotation databases
GeneID	1154497.
GenomeReviews	Gene locus XAC0426 in contig AE008923_GR.
KEGG	xac:XAC0426.
NMPDR	fig|190486.1.peg.425.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	GFDWSVA.
Enzyme and pathway databases
BioCyc	XAXO190486:XAC0426-MONOMER.
BRENDA	2.4.1.18. 289771.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB2_XANAC

Accession

Primary (citable) accession number: Q8PQA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)