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Protein

Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme

Gene

glnE

Organism
Xanthomonas axonopodis pv. citri (strain 306)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the regulation of glutamine synthetase GlnA, a key enzyme in the process to assimilate ammonia. When cellular nitrogen levels are high, the C-terminal adenylyl transferase (AT) inactivates GlnA by covalent transfer of an adenylyl group from ATP to specific tyrosine residue of GlnA, thus reducing its activity. Conversely, when nitrogen levels are low, the N-terminal adenylyl removase (AR) activates GlnA by removing the adenylyl group by phosphorolysis, increasing its activity. The regulatory region of GlnE binds the signal transduction protein PII (GlnB) which indicates the nitrogen status of the cell.UniRule annotation

Catalytic activityi

[Glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine + phosphate = [glutamine synthetase]-L-tyrosine + ADP.UniRule annotation
ATP + [glutamine synthetase]-L-tyrosine = diphosphate + [glutamine synthetase]-O4-(5'-adenylyl)-L-tyrosine.UniRule annotation

Cofactori

Mg2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Nucleotidyltransferase, Transferase
LigandATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeUniRule annotation
Alternative name(s):
ATP:glutamine synthetase adenylyltransferaseUniRule annotation
ATaseUniRule annotation
Including the following 2 domains:
Glutamine synthetase adenylyl-L-tyrosine phosphorylaseUniRule annotation (EC:2.7.7.89UniRule annotation)
Alternative name(s):
Adenylyl removaseUniRule annotation
Short name:
ARUniRule annotation
Short name:
AT-NUniRule annotation
Glutamine synthetase adenylyl transferaseUniRule annotation (EC:2.7.7.42UniRule annotation)
Alternative name(s):
Adenylyl transferaseUniRule annotation
Short name:
ATUniRule annotation
Short name:
AT-CUniRule annotation
Gene namesi
Name:glnEUniRule annotation
Ordered Locus Names:XAC0550
OrganismiXanthomonas axonopodis pv. citri (strain 306)
Taxonomic identifieri190486 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000000576 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002092851 – 941Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzymeAdd BLAST941

Interactioni

Protein-protein interaction databases

STRINGi190486.XAC0550

Structurei

3D structure databases

ProteinModelPortaliQ8PPY3
SMRiQ8PPY3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 437Adenylyl removaseUniRule annotationAdd BLAST437
Regioni444 – 941Adenylyl transferaseUniRule annotationAdd BLAST498

Sequence similaritiesi

Belongs to the GlnE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE6 Bacteria
COG1391 LUCA
HOGENOMiHOG000256491
KOiK00982
OMAiFTVQLLQ
OrthoDBiPOG091H0675

Family and domain databases

HAMAPiMF_00802 GlnE, 1 hit
InterProiView protein in InterPro
IPR023057 GlnE
IPR005190 GlnE_rpt_dom
IPR013546 PII_UdlTrfase/GS_AdlTrfase
PANTHERiPTHR30621 PTHR30621, 1 hit
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 2 hits
PF03710 GlnE, 2 hits

Sequencei

Sequence statusi: Complete.

Q8PPY3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPMPTVSMSP ALTALIERAV ARVRQSLPVE QAWPGGEFDR QLAQVALASE
60 70 80 90 100
FALDTLARQP ALLQHLAQPD PPPLPLPQFD PAQPQLWPAQ LRRYRSAEST
110 120 130 140 150
RLVWRDVLGL DSVEATLAGA TQLAEHCLQC GLQALEQQFH TRHGKVVAAD
160 170 180 190 200
GSVQRLVVFG LGKLGGGELN FSSDVDLVYA YPQGGQSDGA RPLAAEEYFA
210 220 230 240 250
RLGQQLARLL DETTADGFSH RVDLRLRPFG TAGRVALSFA GMDQYFQREG
260 270 280 290 300
RDWERYAWLK ARAVAGDIDA GEAWLETLRP FVYRRYLDFT ALDGLREMKA
310 320 330 340 350
AITAEVARHD RLDDIKRGPG GIREIEFLAQ SLQLIRGGRE PSLRERRLLP
360 370 380 390 400
ALQALVAAGQ IDQENGQALS TAYRFLRRLE NRLQMLRDAQ THALPQAPLD
410 420 430 440 450
RERIALGLGY AQWSALLDAL APQRARVAAE FAELLAPRVR ATAPDALADY
460 470 480 490 500
WRALPDGDAA PLAGIGLNDP DGAHRALADF AQSSGVRALS DSARARLDRV
510 520 530 540 550
MPALLHAATR ASQPDAAVRR MLGLLQATLR RTSYLALLDE QPSALARLVD
560 570 580 590 600
VLSRSALLAE RLAAYPLLLD ELLDTRISGP LPDRAALHAA CAHTLHIDDT
610 620 630 640 650
EAALRELNER RLALSFRIAL ATLDGRQQAV ESTRQLAWLA EAVVQTVLHL
660 670 680 690 700
ARSDMLAAHG HVPGGSFAIV GYGSLGGLEL GFGSDLDLVF LYDHPREVDA
710 720 730 740 750
SDGKRPLDAG RWFARLAQKV MALLAAETGA GRLYDIDVRL RPDGGKGALV
760 770 780 790 800
SSLASYREYQ RDRAWTWEHQ ALVRARAVAG DAALCDAFAQ VRRDTLMRVR
810 820 830 840 850
DTAQLHEDVR KMRARMRAEL DRSDAGRLDL KQGAGGLVDL EFVLQAGVLG
860 870 880 890 900
LAAQQPQLLD ACDTPALIDA LARTHWLPDE SAAPLHQAHA TLVDAGLSCT
910 920 930 940
LDRRPRLIAP TPAIQQARGI IFNAARGQGL TFPLGKDETA L
Length:941
Mass (Da):102,806
Last modified:October 1, 2002 - v1
Checksum:i9249F9740229C2F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008923 Genomic DNA Translation: AAM35439.1
RefSeqiWP_011050383.1, NC_003919.1

Genome annotation databases

EnsemblBacteriaiAAM35439; AAM35439; XAC0550
KEGGixac:XAC0550

Similar proteinsi

Entry informationi

Entry nameiGLNE_XANAC
AccessioniPrimary (citable) accession number: Q8PPY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health