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Q8PN19

- SYI_XANAC

UniProt

Q8PN19 - SYI_XANAC

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xanthomonas axonopodis pv. citri (strain 306)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei577 – 5771Aminoacyl-adenylateUniRule annotation
Binding sitei621 – 6211ATPUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciXAXO190486:GH55-1254-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:XAC1254
OrganismiXanthomonas axonopodis pv. citri (strain 306)
Taxonomic identifieri190486 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000000576: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 943943Isoleucine--tRNA ligasePRO_0000098504Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi190486.XAC1254.

Structurei

3D structure databases

ProteinModelPortaliQ8PN19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi618 – 6225"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8PN19-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQDYKATLH LPATEFPMRG DLPKREPAML ERWEREGFYA QLRANAAGRP
60 70 80 90 100
LFVLHDGPPY ANGQIHLGHA VNKILKDIIV KSKYLAGFDA PYIPGWDCHG
110 120 130 140 150
LPIEIAIEKK YGKVGVKLDA AEFRQKCREY ATEQIDLQRR DFKRLGVIGD
160 170 180 190 200
WDNPYKTLDF RFEANEIRAL AKVVDNGHLT RGVKPVHWCF DCGSALAEAE
210 220 230 240 250
IEYADKVSPT VDIAYPARDP AAVAAAFGAS LPAGVGVAVP IWTTTPWTLP
260 270 280 290 300
ASLAVSLGAE LDYVLVEGPA DRGQPRWLVI AEALAAKALA RYGVGEVVVH
310 320 330 340 350
GHAKGAALEH MLLNHPFYAE REIPLLLGDH VSAEDGTGAV HTAPGHGQED
360 370 380 390 400
YQVSKQYGLL ERYGAAQINP VDGRGVYLPS TPPLRDTVLA GLHIWKANDV
410 420 430 440 450
IIEALRETGV LLAASKMEHS YPHCWRHKTP IAFRATPQWF ISMEQANLRA
460 470 480 490 500
DALKAIENVH WYPSWGQARI AGMVDGRPDW TISRQRTWGV PIALFVHRET
510 520 530 540 550
GEPHPRSTEL LRQVADRVEQ GGVDVWYTLD ASELLSSEAA DYEKITDILD
560 570 580 590 600
VWFDSGVTHE AVLPDRGLPK PADLYLEGSD QHRGWFQSSL LSGVAMDKAA
610 620 630 640 650
PYKQCLTHGF TVDEHGRKMS KSLGNGIEPQ DIMKTLGADI LRLWIASADY
660 670 680 690 700
SNEMSLSQEI LKRNADAYRR LRNTARFLLG NLHGFDPLQH LVALDEMVLL
710 720 730 740 750
DRWIVHRAHE LQEKIVAAYA RYDFAEIVQA LLNFCSVDLG SLYLDVTKDR
760 770 780 790 800
LYTMAEDARG RRSAQSAMYH VAEAFVRWIA PVLSFTAEEL WGYLPGKHVD
810 820 830 840 850
NVLFATWYDG LAPLPADAAL SGADFDKLLV LREQVAKVLE PMRANGAIGA
860 870 880 890 900
ALEAEITVAA DAQTAARWQP LAEELRFLFI SGDVTVTAAS TDDIFVSAQP
910 920 930 940
TTKAKCVRCW HHQASVGSDP RHPELCSRCV SNIEGPGERR RWF
Length:943
Mass (Da):104,739
Last modified:October 1, 2002 - v1
Checksum:i401115611B1AD660
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008923 Genomic DNA. Translation: AAM36126.1.
RefSeqiNP_641590.1. NC_003919.1.
WP_011050802.1. NC_003919.1.

Genome annotation databases

EnsemblBacteriaiAAM36126; AAM36126; XAC1254.
GeneIDi1155325.
KEGGixac:XAC1254.
PATRICi24054481. VBIXanAxo33670_1322.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE008923 Genomic DNA. Translation: AAM36126.1 .
RefSeqi NP_641590.1. NC_003919.1.
WP_011050802.1. NC_003919.1.

3D structure databases

ProteinModelPortali Q8PN19.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 190486.XAC1254.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAM36126 ; AAM36126 ; XAC1254 .
GeneIDi 1155325.
KEGGi xac:XAC1254.
PATRICi 24054481. VBIXanAxo33670_1322.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci XAXO190486:GH55-1254-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparison of the genomes of two Xanthomonas pathogens with differing host specificities."
    da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P.
    , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.
    Nature 417:459-463(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 306.

Entry informationi

Entry nameiSYI_XANAC
AccessioniPrimary (citable) accession number: Q8PN19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3