Reviewed, UniProtKB/Swiss-Prot Q8PLB4 (CHEB1_XANAC)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	XAC1888

Organism

Xanthomonas axonopodis pv. citri (Citrus canker) [Complete proteome] [HAMAP]

Taxonomic identifier

92829 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000158043
Regions
	Domain	8 – 125	118	Response regulatory
	Domain	165 – 357	193	CheB-type methylesterase
Sites
	Active site	177	1	By similarity
	Active site	203	1	By similarity
	Active site	299	1	By similarity
Amino acid modifications
	Modified residue	59	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PLB4-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: DDDBDF1C4E11942F	FASTA	358	38,028
	10 20 30 40 50 60 MTLETPVRVL IVDDSAVVRQ MLTEILSRDA GIEVVGSAAD PLLAREKIKR LNPDVITLDV 70 80 90 100 110 120 EMPRMDGLVF LENLMRLRPT PVVMISSLTE RGADTTLQAL SLGAVDFVSK PKIDVARGLE 130 140 150 160 170 180 GYAEEIVSKV KMAAKAKVSA LNRPSAPKVT LDMQSAPVAG SALRFRTTDR LIAIGASAGG 190 200 210 220 230 240 TEALRVVLEH MPADAPAVVM TQHLPASFST AFAERLNRHS AMSVREATDG EAILPGHAYL 250 260 270 280 290 300 PPGGQHLRII RDGARWRCRI DDGPPVNRHK PAVDVLFRSV AANAGPNAVG AILTGMGDDG 310 320 330 340 350 ARGLLEMLQA GAPTLVQDEA SSVVWGMPGA AYKLGAAQEV VPLDRVAERL LALSAQAR

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 306.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE008923 Genomic DNA. Translation: AAM36750.1.
RefSeq	NP_642214.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHD based on UniProtKB P04042.
ModBase	Search...
Genome annotation databases
GeneID	1155959.
GenomeReviews	Gene locus XAC1888 in contig AE008923_GR.
KEGG	xac:XAC1888.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PLB4.
OMA	IVIAQHI.
Enzyme and pathway databases
BioCyc	XAXO190486:XAC1888-MON.
BRENDA	3.1.1.61. 289771.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_XANAC

Accession

Primary (citable) accession number: Q8PLB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)