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Q8PHZ5 (GSHB_XANAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:XAC3103
OrganismXanthomonas axonopodis pv. citri (strain 306) [Complete proteome] [HAMAP]
Taxonomic identifier190486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Magnesium
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutathione synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197496

Regions

Domain124 – 311188ATP-grasp
Nucleotide binding151 – 20858ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8PHZ5 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: BAEABE5F8A0FF268

FASTA31634,321
        10         20         30         40         50         60 
MSLDVVVVMD PIASIKIAKD TTFAMLLEAQ RRGHRLHYVR PGGLSLREGR AVAQVAPLSV 

        70         80         90        100        110        120 
REDKTSWFTL GEFAELAFGP GQVVLMRKDP PVDAEFVYDT QVLSVAQRAG AQIVNDPQGL 

       130        140        150        160        170        180 
RDYNEKLAAL LFPQCCPPTL VSRDAAALKA FVLEHGQAVL KPLDGMGGRS IFRSGSGDPN 

       190        200        210        220        230        240 
LNVILETLTD GNRKLTLAQR FIPDITAGDK RILLVDGLPV DYCLARIPQG DEFRGNLAAG 

       250        260        270        280        290        300 
GRGEGRPLSE RDRWIAAQVG PEMRRRGMRF VGLDVIGDYL TEVNVTSPTC VRELDAQFGL 

       310 
NIAGLLFDAI EAGAAQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008923 Genomic DNA. Translation: AAM37948.1.
RefSeqNP_643412.1. NC_003919.1.

3D structure databases

ProteinModelPortalQ8PHZ5.
SMRQ8PHZ5. Positions 4-314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190486.XAC3103.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM37948; AAM37948; XAC3103.
GeneID1157174.
KEGGxac:XAC3103.
PATRIC24058341. VBIXanAxo33670_3224.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMADWKIARA.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

BioCycXAXO190486:GH55-3103-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_XANAC
AccessionPrimary (citable) accession number: Q8PHZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways