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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Xanthomonas axonopodis pv. citri (strain 306)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75S-adenosyl-L-methionineUniRule annotation1
Binding sitei100S-adenosyl-L-methionineUniRule annotation1
Binding sitei127S-adenosyl-L-methionineUniRule annotation1
Active sitei150By similarity1
Binding sitei150S-adenosyl-L-methionineUniRule annotation1
Binding sitei154SubstrateUniRule annotation1
Binding sitei186SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:XAC3303
OrganismiXanthomonas axonopodis pv. citri (strain 306)
Taxonomic identifieri190486 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000000576 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001714241 – 261tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST261

Interactioni

Protein-protein interaction databases

STRINGi190486.XAC3303

Structurei

3D structure databases

ProteinModelPortaliQ8PHF4
SMRiQ8PHF4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni156 – 161Interaction with RNAUniRule annotation6
Regioni223 – 226Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q8PHF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDPFTSDGA KMPPKPFTIE EGRRQVRSFV LRQGRFTPAQ QRTFDALWPR
60 70 80 90 100
FGLDYLGAPR DLAATFGRDA HKVLEIGFGN GAALRFAAQQ DPSRDYIGIE
110 120 130 140 150
VHAPGVGRLL NALEEDGSTH VRLYHHDAVE VLEHEIADGA LDEVRIYFPD
160 170 180 190 200
PWHKKRHNKR RLIQPAFAQL LVRKLREGGR LHAATDWADY AEQMWDVLDA
210 220 230 240 250
TPGLVNRAGP RGHVERPTWR PQTHFETRGQ KLGHGVWDLL YDRDSGIGNR
260
ESQEQLPSAS G
Length:261
Mass (Da):29,539
Last modified:October 1, 2002 - v1
Checksum:iE2C84954C459D05C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008923 Genomic DNA Translation: AAM38146.1
RefSeqiWP_011052179.1, NC_003919.1

Genome annotation databases

EnsemblBacteriaiAAM38146; AAM38146; XAC3303
KEGGixac:XAC3303

Similar proteinsi

Entry informationi

Entry nameiTRMB_XANAC
AccessioniPrimary (citable) accession number: Q8PHF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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