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Q8PHF4 (TRMB_XANAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:XAC3303
OrganismXanthomonas axonopodis pv. citri (strain 306) [Complete proteome] [HAMAP]
Taxonomic identifier190486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 261261tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171424

Regions

Region156 – 1616Interaction with RNA Potential
Region223 – 2264Substrate binding Potential

Sites

Active site1501 By similarity
Binding site751S-adenosyl-L-methionine By similarity
Binding site1001S-adenosyl-L-methionine By similarity
Binding site1271S-adenosyl-L-methionine By similarity
Binding site1501S-adenosyl-L-methionine By similarity
Binding site1541Substrate By similarity
Binding site1861Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8PHF4 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: E2C84954C459D05C

FASTA26129,539
        10         20         30         40         50         60 
MTDPFTSDGA KMPPKPFTIE EGRRQVRSFV LRQGRFTPAQ QRTFDALWPR FGLDYLGAPR 

        70         80         90        100        110        120 
DLAATFGRDA HKVLEIGFGN GAALRFAAQQ DPSRDYIGIE VHAPGVGRLL NALEEDGSTH 

       130        140        150        160        170        180 
VRLYHHDAVE VLEHEIADGA LDEVRIYFPD PWHKKRHNKR RLIQPAFAQL LVRKLREGGR 

       190        200        210        220        230        240 
LHAATDWADY AEQMWDVLDA TPGLVNRAGP RGHVERPTWR PQTHFETRGQ KLGHGVWDLL 

       250        260 
YDRDSGIGNR ESQEQLPSAS G 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008923 Genomic DNA. Translation: AAM38146.1.
RefSeqNP_643610.1. NC_003919.1.

3D structure databases

ProteinModelPortalQ8PHF4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190486.XAC3303.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM38146; AAM38146; XAC3303.
GeneID1157374.
KEGGxac:XAC3303.
PATRIC24058727. VBIXanAxo33670_3415.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAQDNFLCC.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycXAXO190486:GH55-3303-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_XANAC
AccessionPrimary (citable) accession number: Q8PHF4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2002
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways