Reviewed, UniProtKB/Swiss-Prot Q8PGR7 (PUR4_XANAC)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	XAC3549

Organism

Xanthomonas axonopodis pv. citri (Citrus canker) [Complete proteome] [HAMAP]

Taxonomic identifier

92829 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	1348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1348	1348	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100424
Regions
	Domain	1099 – 1348	250	Glutamine amidotransferase type-1
	Nucleotide binding	300 – 311	12	ATP Potential
Sites
	Active site	1192	1	Nucleophile By similarity
	Active site	1313	1	By similarity
	Active site	1315	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PGR7-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 4F2ED1E821469A37	FASTA	1,348	144,661
	10 20 30 40 50 60 MMVLEGASAL SPFRRARLET RLQTLVPALR ITGAWHVYFI RPEAGQSPDQ ATLQRILQAN 70 80 90 100 110 120 AAPAERDADA SSRYVVPRLG TLSPWSSKAT ELVRGAGQPI QRVERGTRID LAGWPDDASA 130 140 150 160 170 180 QAAVAKLLHD PMTQSLLGSA AAAEALFNVP DPGQLRRVPL DGLEQANRDL GLALAQDEID 190 200 210 220 230 240 YLRERFGALG RDPADVELMM FAQANSEHCR HKIFNATWTI DGKPQERSLF RMIKHTHQQT 250 260 270 280 290 300 PQHTLSAYSD NAAVVEGVPA ARYRPDPATG QYRSEAVLPS AFAIKVETHN HPTAIAPFPG 310 320 330 340 350 360 AATGAGGEIR DEGATGRGGK PKAGLTGFSV SHLRIPTLPQ PWEAPRALNP RMAPALDIML 370 380 390 400 410 420 DGPLGGAAFN NEFGRPNLLG YFRSFELAEG PGLTRAYDKP IMLAGGLGAI DRNQVEKLRL 430 440 450 460 470 480 QPGDAVIVLG GPAMLIGLGG GAASSVAAGD SAEALDFASV QRENPEMERR CQEVIDRCVA 490 500 510 520 530 540 LGVDNPIRWF HDVGAGGLSN AIPELLHDSG VGGIIDLGRV LTDDPSLSPL ELWCNESQER 550 560 570 580 590 600 YVLGVPQARL EEFAAICARE RCPFAAVGVA TAEERLVVGY GVLDAGNRES GVGNRNGTLV 610 620 630 640 650 660 AADTASHHSP FPTRDSQLPI DLPMDVLFGK APKMHRDAAH PGAPQWPVLQ TASLDLQQAG 670 680 690 700 710 720 LRVLAHPTVA SKSFLVTIGD RSVGGLTARE QMIGPWQLPL ADCAITLAGF DTFEGEAMSI 730 740 750 760 770 780 GERTPLALLN AAASARMAVG EAITNLCAAP VQTLDSIKLS ANWMAAAGHS GEDALLYDAV 790 800 810 820 830 840 RAIGMELCPA LELSVPVGKD SLSMQAQWQV GNRESGIGNG ETPQPSASTI PDSPFPIPGE 850 860 870 880 890 900 TLKSVSPVSL IISAFAPVGD VRTQLTPLLR SGEESELWLI GLGGGKQRLG GSVLAQVYAD 910 920 930 940 950 960 DTALPAFGGE VPDLDDAQRL RSFFELIRDA RDSGLLLAYH DRSDGGAFAA LCEMAFASRQ 970 980 990 1000 1010 1020 GLDITLDAWG DDAFRSLFNE ELGAVVQIAS EDRAAFADLV ERHALTECAQ RIARPTGTPR 1030 1040 1050 1060 1070 1080 IRVSGQGRVL AEWRWEELFD AWWSVTHAMQ KLRDNPDSAD EERALARDFK ASGLRPKLVF 1090 1100 1110 1120 1130 1140 DPSDDVAAPF VATGTRPKVA ILREQGVNGQ IEMAYNFERA GFRPYDVHMS DLIEGRVDLS 1150 1160 1170 1180 1190 1200 AFVGFAACGG FSYGDVLGAG RGWATSILER SALRDAFAAF FARSDTFALG VCNGCQMLSQ 1210 1220 1230 1240 1250 1260 LKDIIPGAEH WPRFLRNRSE QFEARTALLE VVESPSIFLR GMAGSRIPVA VAHGEGRAEF 1270 1280 1290 1300 1310 1320 DSAVDQAAAR VALRYIDGDG AVASQYPLNP NGSPDGITGL TSSDGRVTIL MPHPERTPRS 1330 1340 ANLSWYPVDW GDDSPWLRMF RNARVWCG

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 306.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008923 Genomic DNA. Translation: AAM38392.1.
RefSeq	NP_643856.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1157620.
GenomeReviews	Gene locus XAC3549 in contig AE008923_GR.
KEGG	xac:XAC3549.
NMPDR	fig|190486.1.peg.3500.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335309.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	XAXO190486:XAC3549-MONOMER.
BRENDA	6.3.5.3. 289771.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_XANAC

Accession

Primary (citable) accession number: Q8PGR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)