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Protein

1,4-alpha-glucan branching enzyme GlgB 1

Gene

glgB1

Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei403 – 4031NucleophileBy similarity
Active sitei456 – 4561Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciXCAM190485:GIXZ-135-MONOMER.
UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB 1 (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1
Alpha-(1->4)-glucan branching enzyme 1
Glycogen branching enzyme 1
Short name:
BE 1
Gene namesi
Name:glgB1
Ordered Locus Names:XCC0135
OrganismiXanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
Taxonomic identifieri190485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000001010: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7247241,4-alpha-glucan branching enzyme GlgB 1PRO_0000188766Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi190485.XCC0135.

Structurei

3D structure databases

ProteinModelPortaliQ8PE48.
SMRiQ8PE48. Positions 112-723.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8PE48-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNRWDPGVT RALAEARHGD AFAVLGAHPS TNGRLLRTYH PGAEHVTAVL
60 70 80 90 100
ADGREVPLEA GPEPGLFAGE LPAEGRYRLR IGWPGGTQDT ADPYAFGPLL
110 120 130 140 150
SDFDLHLISE GHHLQLADAL GANAVEVDGV RGTRFAVWAP NASRVAVVGD
160 170 180 190 200
FNSWDARRHP MRLRHQAGVW ELFVPDVGPG AHYKYQLRGP HGHELPAKAD
210 220 230 240 250
PVARRAELAP GTASIVADPT PHQWSDDGWM ATRARRQAHD APMSIYEIHA
260 270 280 290 300
GSWLREEGLD LDWDGLADRL IPYVADMGFT HVELMPVTEH PFGGSWGYQP
310 320 330 340 350
LGLFAPTARF GSPDGFARFV DRCHREGIGV IVDWVPAHFP TDAHGLAHFD
360 370 380 390 400
GTALYEHADP REGFHRDWNT LIYNHGRREV SGFLIASALE FLQRYHVDGL
410 420 430 440 450
RVDAVASMLY RDYSRNAGEW VPNIHGGREN YETIAFLRRL NEVVREHAPG
460 470 480 490 500
AVTIAEESTA WPGVTADVSH GGLGFHYKWN MGWMHDTLHY IGLDPIYRRY
510 520 530 540 550
HHGELTFSMV YAYSERFVLP ISHDEVVHGK GSLLGRMPGD DWQRFANLRA
560 570 580 590 600
YLGFMFTHPG RKLLFMGCEF GQPTEWNHDA GLPWHLLDDA RHRGVQTLVR
610 620 630 640 650
DLNHLYAQYP ALHAHDDDPS GFAWLVGDDA ANSVVAFLRK GKRGDAPVLV
660 670 680 690 700
VINYTPVVQQ GYRLGVPQGG LWREVFNSDA GIYGGANLGN GGAVTAEPQS
710 720
MHGHAQSLPL LLPPLGVIVL APQG
Length:724
Mass (Da):80,088
Last modified:October 1, 2002 - v1
Checksum:i9927812712B93C4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008922 Genomic DNA. Translation: AAM39454.1.
RefSeqiNP_635530.2. NC_003902.1.

Genome annotation databases

EnsemblBacteriaiAAM39454; AAM39454; XCC0135.
GeneIDi1001987.
KEGGixcc:XCC0135.
PATRICi24070825. VBIXanCam115730_0150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008922 Genomic DNA. Translation: AAM39454.1.
RefSeqiNP_635530.2. NC_003902.1.

3D structure databases

ProteinModelPortaliQ8PE48.
SMRiQ8PE48. Positions 112-723.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190485.XCC0135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM39454; AAM39454; XCC0135.
GeneIDi1001987.
KEGGixcc:XCC0135.
PATRICi24070825. VBIXanCam115730_0150.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiDATEVWV.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciXCAM190485:GIXZ-135-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLGB1_XANCP
AccessioniPrimary (citable) accession number: Q8PE48
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: October 1, 2002
Last modified: January 7, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.