Q8PCQ7 (PUR4_XANCP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3

Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	XCC0656

Organism

Xanthomonas campestris pv. campestris

Taxonomic identifier

340 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	1348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer.
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1348	1348	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100425
Regions
	Domain	1099 – 1348	250	Glutamine amidotransferase type-1
	Nucleotide binding	300 – 311	12	ATP Potential
Sites
	Active site	1192	1	Nucleophile By similarity
	Active site	1313	1	By similarity
	Active site	1315	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8PCQ7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 2A4001CBE41D30D4	FASTA	1,348	144,279
	10 20 30 40 50 60 MMVLEGASAL SPFRRARLET RLQTLVPALR LTGAWHVYFI RADAGRTPDQ ATLQRILQAE 70 80 90 100 110 120 PAPAPRDEAA SSRYVVPRLG TLSPWSSKAT ELMRGAGQPI QRVERGTRID LAGWPEGEAD 130 140 150 160 170 180 QAAVAKLLHD PMTQSLLGSA AAAEALFNVP DPGQLERIPL DALEQANGDL GLALAQDEID 190 200 210 220 230 240 YLRERFAALG RDPADVELMM FAQANSEHCR HKIFNASWTI DGKPQERSLF RMIKHTHQQT 250 260 270 280 290 300 PQHTLSAYSD NAAVVEGVPA ARFRPDPATG EYRSEAVVPS AFAIKVETHN HPTAIAPFPG 310 320 330 340 350 360 AATGAGGEIR DEGATGRGGK PKAGLTGFSV SHLRIPTLPQ PWEAPRALNP RMAPALDIML 370 380 390 400 410 420 DGPLGGAAFN NEFGRPNLLG YFRSFELAEG QGLTRAYDKP IMLAGGLGAI DRNQVEKLRL 430 440 450 460 470 480 QPGDAVIVLG GPAMLIGLGG GAASSVAAGD SAEALDFASV QRENPEMERR CQEVIDHCVA 490 500 510 520 530 540 LGTDNPIRWF HDVGAGGLSN AIPELLHDSG VGGVIDLARV PSDDPSLSPL ELWCNESQER 550 560 570 580 590 600 YVLGVPQARL DEFAAICARE RCPFAAVGVA TAEERLVVGY GVFDAANRES GIGNRNSALP 610 620 630 640 650 660 AAEAASAHSL FPTPDSPLPI NLPMDVLFGK APKMHRDAVH PAAPQWPVLQ TGALDLQEAG 670 680 690 700 710 720 LRVLAHPTVA SKSFLVTIGD RSVGGLTARE QMIGPWQLPL ADCAITLAGF ETFDGEAMSI 730 740 750 760 770 780 GERTPLALLN AAASARMAVG EAITNLCAAP VQTLDSIKLS ANWMAAAGHA GEDALLYDAV 790 800 810 820 830 840 RAVGMELCPA LELSIPVGKD SLSMQAQWQV GNGESGIGNG ETPAPSASAI PDSRLPIPGE 850 860 870 880 890 900 TLKSVSPVSL IISAFAPVSD VRTQLTPLLQ REDESELWLI GLGGGKQRLG GSVLAQVYAD 910 920 930 940 950 960 DSALPAFGGE TPDLDDPQRL RQFFELIRDA REGGLLLAYH DRSDGGAFAA LCEMAFASRQ 970 980 990 1000 1010 1020 GLDITLDAWG DDAFRSLFNE ELGAVVQIAN EDRAAFADLV ERHALTECAQ RIARPTGTPR 1030 1040 1050 1060 1070 1080 VRVSGQGRVL AEWRWEALFD AWWSVTHAMQ KLRDNPDSAD EERAVARNFQ APGLRPKLVF 1090 1100 1110 1120 1130 1140 DPSEDVAAPF VATGARPKVA ILREQGVNGQ IEMAYNFERA GFRAFDVHMS DLIEGRVDLA 1150 1160 1170 1180 1190 1200 QFAGFAACGG FSYGDVLGAG RGWATSILER AALRDAFAAF FARSDTFALG VCNGCQMLSQ 1210 1220 1230 1240 1250 1260 LKDIIPGAEH WPRFLRNRSE QFEARTALLE VVESPSILLR GMAGSRIPVA VAHGEGRAEF 1270 1280 1290 1300 1310 1320 DTAVDQAAAR VALRFIDGDG AVASQYPLNP NGSPDGITGL TSTDGRATIL MPHPERTPRS 1330 1340 VNLSWHPAGW GEDSPWLRMF RNARVWCG

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008922 Genomic DNA. Translation: AAM39972.1.
RefSeq	NP_636048.1. NC_003902.1.
3D structure databases
ProteinModelPortal	Q8PCQ7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1001316.
GenomeReviews	Gene locus XCC0656 in contig AE008922_GR.
KEGG	xcc:XCC0656.
PATRIC	24071968. VBIXanCam115730_0719.
Phylogenomic databases
HOGENOM	HBG335309.
OMA	VKAVGME.
ProtClustDB	PRK05297.
Enzyme and pathway databases
BioCyc	XCAM190485:XCC0656-MONOMER.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR022940. PRibForGlyAmidine_synth_bac. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_XANCP

Accession

Primary (citable) accession number: Q8PCQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program