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Protein

D-alanine--D-alanine ligase B

Gene

ddlB

Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi268Magnesium or manganese 1UniRule annotation1
Metal bindingi282Magnesium or manganese 1UniRule annotation1
Metal bindingi282Magnesium or manganese 2UniRule annotation1
Metal bindingi284Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi146 – 201ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciXCAM190485:G1FZM-726-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligase BUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligase BUniRule annotation
D-alanylalanine synthetase BUniRule annotation
Gene namesi
Name:ddlBUniRule annotation
Ordered Locus Names:XCC0727
OrganismiXanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Taxonomic identifieri190485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000001010 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001779091 – 318D-alanine--D-alanine ligase BAdd BLAST318

Interactioni

Protein-protein interaction databases

STRINGi190485.XCC0727

Structurei

3D structure databases

ProteinModelPortaliQ8PCJ8
SMRiQ8PCJ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini117 – 315ATP-graspUniRule annotationAdd BLAST199

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011592
KOiK01921
OMAiMQGLLEC

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q8PCJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTQIAPARI SDPAAFGRVA VLLGGTSSER EVSLNSGSNV LDALRARGVD
60 70 80 90 100
AQPVDGIPAL AQALVAQRFD RVFNVLHGHN GGGEDGIVQG LMEAFGVPYT
110 120 130 140 150
GSNVLGSALS MDKIRTKQVW LSLGLSTPRY ARLAAGASAQ QIHAAAEQIG
160 170 180 190 200
LPVIVKPANE GSSVGVSRVF DQAQLDEAVT LAARYDGALL MEQLIEGDEL
210 220 230 240 250
TVAVLGDVAL PSIRIVPKGQ WYDYNAKYIA EDTQYLCPGL DGDAEAQIGQ
260 270 280 290 300
LALDAFRAAG CSGWGRVDVM RDGSTGQLYL LEVNTAPGMT SHSLVPKAAR
310
QLGIDFEALV WRVLEQTL
Length:318
Mass (Da):33,569
Last modified:October 1, 2002 - v1
Checksum:i82B6E6AEEB93E1A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008922 Genomic DNA Translation: AAM40042.1
RefSeqiNP_636118.1, NC_003902.1
WP_011035964.1, NC_003902.1

Genome annotation databases

EnsemblBacteriaiAAM40042; AAM40042; XCC0727
GeneIDi1001363
35547194
KEGGixcc:XCC0727
PATRICifig|190485.4.peg.791

Similar proteinsi

Entry informationi

Entry nameiDDLB_XANCP
AccessioniPrimary (citable) accession number: Q8PCJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: October 1, 2002
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health