Reviewed, UniProtKB/Swiss-Prot Q8PBL8 (DNAE2_XANCP)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	XCC1101

Organism

Xanthomonas campestris pv. campestris [Complete proteome] [HAMAP]

Taxonomic identifier

340 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	1082 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1082	1082	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103405

Sequences

	Sequence		Length	Mass (Da)
	Q8PBL8-1 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 1E393E9F4C775871	FASTA	1,082	120,079
	10 20 30 40 50 60 MSWDDAIDGV DRDTPGGRMP RGWTVAARLR AANDDITHAA VADTLPAYAE LHCLSDFSFL 70 80 90 100 110 120 RGASSAEQLF ARAHHCGYSA LAITDECSLA GIVRGLEASR ATGVQLIVGS EFTLVDGTRF 130 140 150 160 170 180 VLLVENAHGY PQLCSVITTG RRAAGKGAYR LGRAEVEAHF RDVVPGVFAL WLPGDQPQAE 190 200 210 220 230 240 QGAWLQRVFA ERAFLAVELH REQDDAARLQ ALQALAQQLG MSALASGDVQ MAQRRDRIVQ 250 260 270 280 290 300 DTLTAIRHTL PLADCGAHLF RNGERHLRPR RALGNIYPHA LLQASVELAQ RCTFDLSKVQ 310 320 330 340 350 360 YTYPRELVPQ GHTPASYLRQ LTEAGMRERW PEGAPAQVVA QIDSELELIA YKGYEAFFLT 370 380 390 400 410 420 VQDVVRFARA QGILCQGRGS SANSAVCYAL GITAVNPSET RLLMARFLSK ERDEPPDIDV 430 440 450 460 470 480 DFEHERREEV LQYVYTKYGR ERAALAATVI CYRGKSAVRD VAKAFGLPPD QIALLANCYG 490 500 510 520 530 540 WGNGDTPMEQ RIAEAGFDLA NPLINKILAV TEHLRDHPRH LSQHVGGFVI SDEPLSMLVP 550 560 570 580 590 600 VENAAMADRT IIQWDKDDLE TMKLLKVDCL ALGMLTCIRK TLDLVRGHRG RDYTIATLPG 610 620 630 640 650 660 EDAATYKMIQ RADTVGVFQI ESRAQMAMLP RLKPREFYDL VIEVAIVRPG PIQGDMVHPY 670 680 690 700 710 720 LRRRQGYEPV SFPSPGVEEI LGRTLGIPLF QEQVMELVIH AGYTDSEADQ LRRSMAAWRR 730 740 750 760 770 780 GGDMEPHRVR IRELMAGRGY APEFIDQIFE QIKGFGSYGF PQSHAASFAK LVYASCWLKR 790 800 810 820 830 840 HEPAAFACGL LNAQPMGFYS ASQIVQDARR GSPERQRVEV LPVDVLHSDW DNILVGGRPW 850 860 870 880 890 900 HSDADPGEQP AIRLGLRQVS GLSEKVVERI VAARAQRPFA DIGDLCLRAA LDEKARLALA 910 920 930 940 950 960 EAGALQSMVG NRNAARWAMA GVEARRPLLP GSPAERAVEL PAPRAGEEIL ADYRAVGLSL 970 980 990 1000 1010 1020 RQHPMALLRP QMLQRRILGL RELQARRHGS GVHVAGLVTQ RQRPATAKGT IFVTLEDEHG 1030 1040 1050 1060 1070 1080 MINVIVWSHL AMRRRRALLE SRLLAVRGRW ERVDGVEHLI AGDLYDLSDL LGEMQLPSRD FH

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE012208 Genomic DNA. Translation: AAM40400.1. Different initiation.
RefSeq	NP_636476.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1000905.
GenomeReviews	Gene locus XCC1101 in contig AE008922_GR.
KEGG	xcc:XCC1101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8PBL8.
Enzyme and pathway databases
BioCyc	XCAM190485:XCC1101-MON.
BRENDA	2.7.7.7. 281360.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_XANCP

Accession

Primary (citable) accession number: Q8PBL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)