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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
LigandMagnesium

Enzyme and pathway databases

BioCyciXCAM190485:G1FZM-1381-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:XCC1383
OrganismiXanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Taxonomic identifieri190485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000001010 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001927771 – 869Bifunctional uridylyltransferase/uridylyl-removing enzymeAdd BLAST869

Interactioni

Protein-protein interaction databases

STRINGi190485.XCC1383

Structurei

3D structure databases

ProteinModelPortaliQ8PAU4
SMRiQ8PAU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini450 – 572HDPROSITE-ProRule annotationAdd BLAST123
Domaini692 – 771ACT 1UniRule annotationAdd BLAST80
Domaini798 – 869ACT 2UniRule annotationAdd BLAST72

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 332UridylyltransferaseAdd BLAST332
Regioni333 – 691Uridylyl-removingAdd BLAST359

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105E1P Bacteria
COG2844 LUCA
HOGENOMiHOG000261778
KOiK00990
OMAiARICGYF

Family and domain databases

CDDicd00077 HDc, 1 hit
HAMAPiMF_00277 PII_uridylyl_transf, 1 hit
InterProiView protein in InterPro
IPR002912 ACT_dom
IPR003607 HD/PDEase_dom
IPR006674 HD_domain
IPR013546 PII_UdlTrfase/GS_AdlTrfase
IPR002934 Polymerase_NTP_transf_dom
IPR010043 UTase/UR
PANTHERiPTHR13734:SF45 PTHR13734:SF45, 2 hits
PfamiView protein in Pfam
PF08335 GlnD_UR_UTase, 1 hit
PF01966 HD, 1 hit
PF01909 NTP_transf_2, 1 hit
PIRSFiPIRSF006288 PII_uridyltransf, 1 hit
SMARTiView protein in SMART
SM00471 HDc, 1 hit
TIGRFAMsiTIGR01693 UTase_glnD, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 2 hits
PS51831 HD, 1 hit

Sequencei

Sequence statusi: Complete.

Q8PAU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTATPADRPD PGVAGDADWA AEARPLLVHA DMRLCKRFDQ GEPTERLLAL
60 70 80 90 100
RARAVDQLMR NAWTRCIPAD AGLSLHAVGG YGRGELFPRS DVDLLVLGET
110 120 130 140 150
AAQQRHEQAL ARLFALLWDV GLPISHAVRS PAQCTSAAAD QTVLTALIES
160 170 180 190 200
RPLVADAQAR AALAAAIAPQ QVWPPRAFFQ AKREELHARH QRFGDTADNL
210 220 230 240 250
EPDIKDGPGG LRDLQTLGWM ALRAFGVKDL EALVGLGHVG MDEAAALRRE
260 270 280 290 300
REELARLRYG LHLVANRPEE RLRFDYQKTL AERLGFADDP ESLGVEKMMQ
310 320 330 340 350
RFYRSAALIR RISDRLLQRF EEQFDGEAVP VQLDAGFSLR RGYLTADADT
360 370 380 390 400
WPDGDVVQVF ALFAQWAAHR EVRGLHSLTA RALAEVLRDL PAYDVADAIA
410 420 430 440 450
RDRFMALLRG PRAVETLNRM ARLGVLGQWI PAFASVSGRM QFDLFHVYTV
460 470 480 490 500
DQHTLMVLRN IALFAAGRAD ERFSITHEVW PRLRKPELLL LAGLFHDIAK
510 520 530 540 550
GRGGDHSELG AVDARAFCLA HRLSEGDTEL VTWLVEQHLR MSVTAQKQDI
560 570 580 590 600
SDPEVIHRFA TLVGTRERLD YLYLLTCADI AGTSPKLWNA WKDRLLADLY
610 620 630 640 650
FAARRALREG LEHPPPREER LREARESART LMQAQGHDDA TIDRQFAGMP
660 670 680 690 700
DENFLRFRPE QLAWQAASLI EVQIGQTLVK ARRAVPDNDA LEVFVYSPDR
710 720 730 740 750
DGLFSAIVAT LDRKGYGIHR ARVLDAPHDA IFDVFEVLPQ DSSADGDPQR
760 770 780 790 800
LAAALRQVLA GDLLKVRPSR RAVPRQLRHF RFAPRVEFSE SAGGRRTRIS
810 820 830 840 850
LVAPDRPGLL ADVAHVLRMQ HLRVHDARIA TFGERAEDQF QITDEHDRPL
860
PDAARQALRD ALCACLDPT
Length:869
Mass (Da):97,246
Last modified:October 10, 2002 - v1
Checksum:iBC86E2D0BBB9159B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008922 Genomic DNA Translation: AAM40681.1
RefSeqiNP_636757.1, NC_003902.1
WP_011036575.1, NC_003902.1

Genome annotation databases

EnsemblBacteriaiAAM40681; AAM40681; XCC1383
GeneIDi1001802
KEGGixcc:XCC1383
PATRICifig|190485.4.peg.1486

Similar proteinsi

Entry informationi

Entry nameiGLND_XANCP
AccessioniPrimary (citable) accession number: Q8PAU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: February 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome