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Protein

N-acetylornithine carbamoyltransferase

Gene

argF'

Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of N-acetylornithine to N-acetylcitrulline in an alternative arginine biosynthesis pathway. The enzyme has no activity with ornithine.

Catalytic activityi

Carbamoyl phosphate + N(2)-acetyl-L-ornithine = phosphate + N-acetyl-L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in the pathway L-arginine biosynthesis, which is part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112Carbamoyl phosphate1
Binding sitei148Carbamoyl phosphate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12073.
BRENDAi2.1.3.9. 6708.
UniPathwayiUPA00068.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylornithine carbamoyltransferase (EC:2.1.3.9)
Short name:
AOTCase
Gene namesi
Name:argF'
Ordered Locus Names:XCC2249
OrganismiXanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Taxonomic identifieri190485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000001010 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001132681 – 339N-acetylornithine carbamoyltransferaseAdd BLAST339

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi190485.XCC2249.

Structurei

Secondary structure

1339
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 11Combined sources3
Helixi14 – 29Combined sources16
Turni35 – 38Combined sources4
Beta strandi40 – 47Combined sources8
Helixi50 – 62Combined sources13
Beta strandi66 – 70Combined sources5
Helixi72 – 75Combined sources4
Beta strandi82 – 84Combined sources3
Helixi95 – 105Combined sources11
Beta strandi107 – 112Combined sources6
Helixi120 – 123Combined sources4
Turni124 – 126Combined sources3
Helixi127 – 135Combined sources9
Beta strandi140 – 146Combined sources7
Helixi149 – 163Combined sources15
Beta strandi172 – 177Combined sources6
Helixi187 – 198Combined sources12
Beta strandi202 – 206Combined sources5
Helixi210 – 212Combined sources3
Helixi216 – 229Combined sources14
Beta strandi232 – 236Combined sources5
Helixi239 – 243Combined sources5
Beta strandi247 – 252Combined sources6
Helixi257 – 259Combined sources3
Helixi266 – 270Combined sources5
Helixi271 – 276Combined sources6
Helixi280 – 284Combined sources5
Beta strandi286 – 288Combined sources3
Beta strandi290 – 292Combined sources3
Turni299 – 301Combined sources3
Helixi305 – 308Combined sources4
Helixi315 – 333Combined sources19

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei28Important for structural integrityBy similarity1
Sitei161Important for structural integrityBy similarity1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KZCX-ray2.20A1-339[»]
3KZKX-ray1.90A1-339[»]
3KZMX-ray1.95A1-339[»]
3KZNX-ray1.80A1-339[»]
3KZOX-ray1.90A1-339[»]
3L02X-ray2.30A1-339[»]
3L04X-ray2.50A1-339[»]
3L05X-ray2.80A1-339[»]
3L06X-ray2.81A1-339[»]
3M4JX-ray2.20A1-339[»]
3M4NX-ray1.90A1-339[»]
3M5CX-ray1.85A1-339[»]
3M5DX-ray2.20A1-339[»]
ProteinModelPortaliQ8P8J2.
SMRiQ8P8J2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8P8J2.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 53Carbamoyl phosphate binding5
Regioni293 – 296N-acetylornithine binding4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK09065.
OMAiSAREHPC.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8P8J2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLKHFLNTQ DWSRAELDAL LTQAALFKRN KLGSELKGKS IALVFFNPSM
60 70 80 90 100
RTRTSFELGA FQLGGHAVVL QPGKDAWPIE FNLGTVMDGD TEEHIAEVAR
110 120 130 140 150
VLGRYVDLIG VRAFPKFVDW SKDREDQVLK SFAKYSPVPV INMETITHPC
160 170 180 190 200
QELAHALALQ EHFGTPDLRG KKYVLTWTYH PKPLNTAVAN SALTIATRMG
210 220 230 240 250
MDVTLLCPTP DYILDERYMD WAAQNVAESG GSLQVSHDID SAYAGADVVY
260 270 280 290 300
AKSWGALPFF GNWEPEKPIR DQYQHFIVDE RKMALTNNGV FSHCLPLRRN
310 320 330
VKATDAVMDS PNCIAIDEAE NRLHVQKAIM AALVGQSRP
Length:339
Mass (Da):37,873
Last modified:October 1, 2002 - v1
Checksum:i8FEFBA16773A00D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008922 Genomic DNA. Translation: AAM41528.1.
RefSeqiNP_637604.1. NC_003902.1.
WP_011037393.1. NC_003902.1.

Genome annotation databases

EnsemblBacteriaiAAM41528; AAM41528; XCC2249.
GeneIDi998828.
KEGGixcc:XCC2249.
PATRICi24075403. VBIXanCam115730_2399.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008922 Genomic DNA. Translation: AAM41528.1.
RefSeqiNP_637604.1. NC_003902.1.
WP_011037393.1. NC_003902.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3KZCX-ray2.20A1-339[»]
3KZKX-ray1.90A1-339[»]
3KZMX-ray1.95A1-339[»]
3KZNX-ray1.80A1-339[»]
3KZOX-ray1.90A1-339[»]
3L02X-ray2.30A1-339[»]
3L04X-ray2.50A1-339[»]
3L05X-ray2.80A1-339[»]
3L06X-ray2.81A1-339[»]
3M4JX-ray2.20A1-339[»]
3M4NX-ray1.90A1-339[»]
3M5CX-ray1.85A1-339[»]
3M5DX-ray2.20A1-339[»]
ProteinModelPortaliQ8P8J2.
SMRiQ8P8J2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi190485.XCC2249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM41528; AAM41528; XCC2249.
GeneIDi998828.
KEGGixcc:XCC2249.
PATRICi24075403. VBIXanCam115730_2399.

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK09065.
OMAiSAREHPC.

Enzyme and pathway databases

UniPathwayiUPA00068.
BioCyciMetaCyc:MONOMER-12073.
BRENDAi2.1.3.9. 6708.

Miscellaneous databases

EvolutionaryTraceiQ8P8J2.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00101. ATCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAOTC_XANCP
AccessioniPrimary (citable) accession number: Q8P8J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.