ID   GSHB_XANCP              Reviewed;         316 AA.
AC   Q8P6P1;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 43.
DE   RecName: Full=Glutathione synthetase;
DE            EC=6.3.2.3;
DE   AltName: Full=Glutathione synthase;
DE   AltName: Full=GSH synthetase;
DE            Short=GSH-S;
DE            Short=GSHase;
GN   Name=gshB; OrderedLocusNames=XCC2926;
OS   Xanthomonas campestris pv. campestris.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=340;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33913 / NCPPB 528 / LMG 568;
RX   MEDLINE=22022145; PubMed=12024217; DOI=10.1038/417459a;
RA   da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R.,
RA   Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A.,
RA   Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C.,
RA   Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F.,
RA   Ciapina L.P., Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R.,
RA   El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C.,
RA   Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A.,
RA   Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F.,
RA   Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M.,
RA   Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H.,
RA   Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R.,
RA   Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F.,
RA   Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D.,
RA   Trindade dos Santos M., Truffi D., Tsai S.M., White F.F.,
RA   Setubal J.C., Kitajima J.P.;
RT   "Comparison of the genomes of two Xanthomonas pathogens with differing
RT   host specificities.";
RL   Nature 417:459-463(2002).
CC   -!- CATALYTIC ACTIVITY: ATP + gamma-L-glutamyl-L-cysteine + glycine =
CC       ADP + phosphate + glutathione.
CC   -!- COFACTOR: Binds 1 magnesium or manganese ion per subunit (By
CC       similarity).
CC   -!- PATHWAY: Sulfur metabolism; glutathione biosynthesis; glutathione
CC       from L-cysteine and L-glutamate: step 2/2.
CC   -!- SIMILARITY: Belongs to the prokaryotic GSH synthase family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; AE012407; AAM42198.1; -; Genomic_DNA.
DR   RefSeq; NP_638274.1; -.
DR   HSSP; P04425; 1GSH.
DR   GeneID; 999889; -.
DR   GenomeReviews; AE008922_GR; XCC2926.
DR   KEGG; xcc:XCC2926; -.
DR   HOGENOM; Q8P6P1; -.
DR   OMA; Q8P6P1; VRNAPEK.
DR   BioCyc; XCAM190485:XCC2926-MON; -.
DR   BRENDA; 6.3.2.3; 281360.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004363; F:glutathione synthase activity; IEA:HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006750; P:glutathione biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00162; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR006284; Glut_synth_pro.
DR   InterPro; IPR004218; GSHS_ATP_bd.
DR   InterPro; IPR004215; GSHS_N.
DR   InterPro; IPR013817; Pre-ATP_grasp.
DR   Gene3D; G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
DR   Gene3D; G3DSA:3.40.50.20; Pre-ATP_grasp; 1.
DR   Pfam; PF02955; GSH-S_ATP; 1.
DR   Pfam; PF02951; GSH-S_N; 1.
DR   TIGRFAMs; TIGR01380; glut_syn; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glutathione biosynthesis; Ligase;
KW   Magnesium; Manganese; Metal-binding; Nucleotide-binding.
FT   CHAIN         1    316       Glutathione synthetase.
FT                                /FTId=PRO_0000197497.
FT   DOMAIN      124    311       ATP-grasp.
FT   NP_BIND     151    208       ATP (By similarity).
FT   METAL       282    282       Magnesium or manganese (By similarity).
FT   METAL       284    284       Magnesium or manganese (By similarity).
SQ   SEQUENCE   316 AA;  34173 MW;  B6818474D0D17641 CRC64;
     MSLDVVVVMD PIASIKIAKD TTFAMLLEAQ RRGHRLHYVR PGGLSLHEGR AVAQVAPLSV
     REDKASWFTL GAFTELVFGP GQVVLMRKDP PVDAEFIYDT QVLAVAQRAG AQVVNDPQGL
     RDYNEKLAAL LFPQCCPPTL VSRDAAALKA FVLAHGQAVL KPLDGMGGRS IFRSGTGDPN
     LNVILETLTD GGRKLTLAQR FIPDITAGDK RILLVDGEPV DYCLARIPQG DEFRGNLAAG
     GRGEGRPLSE RDRWIAAQVG PEMKRRGMRF VGLDVIGDYL TEVNVTSPTC VRELDAQFGL
     NIAGLLFDAI EAGTAQ
//