Q8P607 (CYSH_XANCP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	XCC3175

Organism

Xanthomonas campestris pv. campestris

Taxonomic identifier

340 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100657

Sequences

	Sequence		Length	Mass (Da)
	Q8P607 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 6CB195BA3A561C26	FASTA	241	27,637
	10 20 30 40 50 60 MTALPATSIA APSLDDLDAL NAHLETLRAD ERVAWALQHG PQQAALSSSF GAQSAVTLHL 70 80 90 100 110 120 LTQQRPDIPV ILIDTGYLFP ETYRFADALT ERLSLNLQVY RPLVSRAWME ARHGRLWEQG 130 140 150 160 170 180 MVGIDQYNNL RKVEPMRRAL DELNVGTWFT GLRRSQSGGR AQTPIVQKRG DRYKISPIAD 190 200 210 220 230 240 WTDRDVWQYL QAHALPYHPL WEQGYVSIGD FHTTRRWEPG MREEDTRFFG LKRECGIHED I

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008922 Genomic DNA. Translation: AAM42445.1.
RefSeq	NP_638521.1. NC_003902.1.
3D structure databases
ProteinModelPortal	Q8P607.
SMR	Q8P607. Positions 12-212.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	999271.
GenomeReviews	Gene locus XCC3175 in contig AE008922_GR.
KEGG	xcc:XCC3175.
PATRIC	24077409. VBIXanCam115730_3391.
Phylogenomic databases
HOGENOM	HBG758022.
OMA	AIHGTRF.
ProtClustDB	PRK02090.
Enzyme and pathway databases
BioCyc	XCAM190485:XCC3175-MONOMER.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase_CysH. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMs	TIGR00434. CysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_XANCP

Accession

Primary (citable) accession number: Q8P607

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program