Reviewed, UniProtKB/Swiss-Prot Q8P5D7 (BETA_XANCP)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	XCC3404

Organism

Xanthomonas campestris pv. campestris [Complete proteome] [HAMAP]

Taxonomic identifier

340 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Choline dehydrogenase HAMAP MF_00750		PRO_0000205609
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8P5D7-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 12409448073F6478	FASTA	556	61,445
	10 20 30 40 50 60 MQREYDYIII GAGSAGNVLA ARLTEDPGVS VLLLEAGGPD YRLDFRTQMP AALAFPLQGR 70 80 90 100 110 120 RYNWAYETEP EPHMDNRRME CGRGKGLGGS SLINGMCYIR GNALDFDHWA KRPGLEDWGY 130 140 150 160 170 180 RDVLPYFRKA ETRDIGANDY HGGEGPVSVA TPKNDNNVLF QAMVDAGVQA GYPRTDDLNG 190 200 210 220 230 240 YQQEGFGPMD RTVTPQGRRA STARGYLDMA KPRDSLHIVT HATTDRILFA GKRAVGVHYL 250 260 270 280 290 300 VGNSSEGIDA HARREVLVCA GAIASPQLLQ RSGVGAPDLL RALDVQLVHD LPGVGQNLQD 310 320 330 340 350 360 HLEVYMQYAC TKPVSLYPAL QWWNQPAIGA EWLFAGTGTG ASNQFEAGGF IRTREEFDWP 370 380 390 400 410 420 NIQYHFLPVA INYNGSNAVK EHGFQAHVGS MRTPSRGRVH ARSRDPRQHP SILFNYQSTD 430 440 450 460 470 480 QDWQEFRDAI RITREIIAQP ALDPYRGREI SPSADCKTDA ELDAFVRARA ETAYHPSCSC 490 500 510 520 530 540 AMGTDDMAVV DGQGRVHGME GLRVIDASIM PRIITGNLNA TTIMIAEKIV DRIRGRAPLP 550 RSTADYYVAG DAPVRR

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE012459 Genomic DNA. Translation: AAM42674.1.
RefSeq	NP_638750.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	999936.
GenomeReviews	Gene locus XCC3404 in contig AE008922_GR.
KEGG	xcc:XCC3404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8P5D7.
OMA	Q8P5D7. GRRMECG.
Enzyme and pathway databases
BioCyc	XCAM190485:XCC3404-MON.
BRENDA	1.1.99.1. 281360.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_XANCP

Accession

Primary (citable) accession number: Q8P5D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)