Q8P3Z4 (SYW_XANCP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	XCC3923

Organism

Xanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568) [Reference proteome] [HAMAP]

Taxonomic identifier

190485 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas › Xanthomonas campestris › Xanthomonas campestris pv. campestris

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Tryptophan--tRNA ligase HAMAP-Rule MF_00140		PRO_0000136713
Regions
	Motif	13 – 21	9	"HIGH" region HAMAP-Rule MF_00140
	Motif	204 – 208	5	"KMSKS" region HAMAP-Rule MF_00140
Sites
	Binding site	207	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8P3Z4 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 1043117CB2239037	FASTA	431	47,101
	10 20 30 40 50 60 MQMTTRVLTG ITPSGTPHLG NYVGAIRPAI QASTATDAES FYFLADLHSL IKAQDPARTQ 70 80 90 100 110 120 RSTLEIAASW LACGLDPDKV WFYRQSDVPE TTELMWLLTC VAGKGILNRA HAYKAAMDKN 130 140 150 160 170 180 RAEGEDEDAG VTAGLFMYPV LMAADILIFN AHKVPVGRDQ IQHIEMARDF AQRFNHVYGQ 190 200 210 220 230 240 DYFTLPDAVI DEQVATLPGL DGRKMSKSYN NTIPLFAPRE ELRKLVFSIL TDSRAPGEAK 250 260 270 280 290 300 DTQGSALFQL YQAFATPQET AAFAQAFADG ISWADAKQQL FERIDLEIAP LRARYEALMA 310 320 330 340 350 360 EPAKIEATLR AGGARLRARY ATPLLAQLRD AVGLRDLSSQ ASAVGPATAV KAALPVFKQY 370 380 390 400 410 420 RESDGQFYFK LHDAAGTLLL QSDGFASPRE AGQLIARLKQ AEDTTQLQLP GVQLPADAAP 430 VLAALRALRE A

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008922 Genomic DNA. Translation: AAM43145.1.
RefSeq	NP_639263.1. NC_003902.1.
3D structure databases
ProteinModelPortal	Q8P3Z4.
ModBase	Search...
Protein-protein interaction databases
STRING	190485.XCC3923.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM43145; AAM43145; XCC3923.
GeneID	1000585.
KEGG	xcc:XCC3923.
PATRIC	24079041. VBIXanCam115730_4198.
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059939.
KO	K01867.
OMA	ISWADAK.
ProtClustDB	PRK12284.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_XANCP

Accession

Primary (citable) accession number: Q8P3Z4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program