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Q8P1W3 (TPIS_STRP8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1
Alternative name(s):
Triose-phosphate isomerase
Gene names
Name:tpiA
Ordered Locus Names:spyM18_0680
OrganismStreptococcus pyogenes serotype M18 (strain MGAS8232) [Complete proteome] [HAMAP]
Taxonomic identifier186103 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus ›

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP-Rule MF_00147

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP-Rule MF_00147

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP-Rule MF_00147

Subunit structure

Homodimer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
   Biological processGluconeogenesis
Glycolysis
Pentose shunt
   Cellular componentCytoplasm
   Molecular functionIsomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: HAMAP

glycolysis

Inferred from electronic annotation. Source: HAMAP

pentose-phosphate shunt

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontriose-phosphate isomerase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 252251Triosephosphate isomerase HAMAP-Rule MF_00147
PRO_0000090302

Sites

Active site961Electrophile By similarity
Active site1681Proton acceptor By similarity
Binding site101Substrate By similarity
Binding site121Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8P1W3 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 62EC992D86E9B00B

FASTA25226,604
        10         20         30         40         50         60 
MSRKPIIAGN WKMNKNPQEA KAFVEAVASK LPSTDLVDVA VAAPAVDLVT TIEAAKDSVL 

        70         80         90        100        110        120 
KVAAQNCYFE NTGAFTGETS PKVLAEMGAD YVVIGHSERR DYFHETDEDI NKKAKAIFAN 

       130        140        150        160        170        180 
GLTPIICCGE SLETYEAGKA AEFVGAQVSA ALAGLSAEQV ASLVLAYEPI WAIGTGKSAT 

       190        200        210        220        230        240 
QDDAQNMCKA VRDVVAADFG QEVADKVRVQ YGGSVKPENV KDYMACPDVD GALVGGASLE 

       250 
ADSFLALLDF LN

« Hide

References

[1]"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks."
Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M.
Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MGAS8232.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE009949 Genomic DNA. Translation: AAL97355.1.
RefSeqNP_606856.1. NC_003485.1.

3D structure databases

ProteinModelPortalQ8P1W3.
SMRQ8P1W3. Positions 3-250.
ModBaseSearch...

Protein-protein interaction databases

STRING186103.spyM18_0680.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL97355; AAL97355; spyM18_0680.
GeneID994030.
KEGGspm:spyM18_0680.
PATRIC19747991. VBIStrPyo4396_0587.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0149.
HOGENOMHOG000226412.
KOK01803.
OMAISGQWPR.
ProtClustDBPRK00042.

Enzyme and pathway databases

UniPathwayUPA00109; UER00189.
UPA00138.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00147_B. TIM_B.
InterProIPR013785. Aldolase_TIM.
IPR022896. TrioseP_Isoase_bac/euk.
IPR000652. Triosephosphate_isomerase.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERPTHR21139. PTHR21139. 1 hit.
PfamPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMSSF51351. Triophos_ismrse. 1 hit.
TIGRFAMsTIGR00419. tim. 1 hit.
PROSITEPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTPIS_STRP8
AccessionPrimary (citable) accession number: Q8P1W3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: January 23, 2007
Last modified: May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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