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Q8P1M4 (GYRB_STRP8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA gyrase subunit B

EC=5.99.1.3
Gene names
Name:gyrB
Ordered Locus Names:spyM18_0795
OrganismStreptococcus pyogenes serotype M18 (strain MGAS8232) [Complete proteome] [HAMAP]
Taxonomic identifier186103 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length650 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01898

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01898

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity.

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 650650DNA gyrase subunit B HAMAP-Rule MF_01898
PRO_0000145352

Regions

Domain429 – 543115Toprim

Sites

Metal binding4351Magnesium 1; catalytic By similarity
Metal binding5081Magnesium 1; catalytic By similarity
Metal binding5081Magnesium 2 By similarity
Metal binding5101Magnesium 2 By similarity
Site4601Interaction with DNA By similarity
Site4631Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8P1M4 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 2CF598897B872ACB

FASTA65072,309
        10         20         30         40         50         60 
MIEENKHFEK KMQEYDASQI QVLEGLEAVR MRPGMYIGST AKEGLHHLVW EIVDNSIDEA 

        70         80         90        100        110        120 
LAGFASHIKV FIEADNSITV VDDGRGIPVD IQAKTGRPAV ETVFTVLHAG GKFGGGGYKV 

       130        140        150        160        170        180 
SGGLHGVGSS VVNALSTQLD VRVYKNGQIH YQEFKRGAVV ADLEIIGTTD VTGTTVHFTP 

       190        200        210        220        230        240 
DPEIFTETTQ FDYSVLAKRI QELAFLNRGL KISITDKRSG MEQEEHFHYE GGIGSYVEFL 

       250        260        270        280        290        300 
NDKKDVIFET PIYTDGELEG IAVEVAMQYT TSYQETVMSF ANNIHTHEGG THEQGFRAAL 

       310        320        330        340        350        360 
TRVINDYAKK NKILKENEDN LTGEDVREGL TAVISVKHPN PQFEGQTKTK LGNSEVVKIT 

       370        380        390        400        410        420 
NRLFSEAFQR FLLENPQVAR KIVEKGILAS KARIAAKRAR EVTRKKSGLE ISNLPGKLAD 

       430        440        450        460        470        480 
CSSNDANQNE LFIVEGDSAG GSAKSGRNRE FQAILPIRGK ILNVEKATMD KILANEEIRS 

       490        500        510        520        530        540 
LFTAMGTGFG ADFDVSKARY QKLVIMTDAD VDGAHIRTLL LTLIYRFMRP VLEAGYVYIA 

       550        560        570        580        590        600 
QPPIYGVKVG SEIKEYIQPG IDQEDQLKTA LEKYSIGRSK PTVQRYKGLG EMDDHQLWET 

       610        620        630        640        650 
TMDPENRLMA RVTVDDAAEA DKVFDMLMGD RVEPRRDFIE ENAVYSTLDI 

« Hide

References

[1]"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks."
Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M.
Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MGAS8232.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE009949 Genomic DNA. Translation: AAL97459.1.
RefSeqNP_606960.1. NC_003485.1.

3D structure databases

ProteinModelPortalQ8P1M4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186103.spyM18_0795.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL97459; AAL97459; spyM18_0795.
GeneID994970.
KEGGspm:spyM18_0795.
PATRIC19748210. VBIStrPyo4396_0695.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0187.
HOGENOMHOG000075154.
KOK02470.
OMAHIIIMTD.
OrthoDBEOG6P334W.

Enzyme and pathway databases

BioCycSPYO186103:GHJG-679-MONOMER.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPMF_01898. GyrB.
InterProIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01059. gyrB. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGYRB_STRP8
AccessionPrimary (citable) accession number: Q8P1M4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2002
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families