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Q8P1M4

- GYRB_STRP8

UniProt

Q8P1M4 - GYRB_STRP8

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Protein

DNA gyrase subunit B

Gene

gyrB

Organism
Streptococcus pyogenes serotype M18 (strain MGAS8232)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotation, Ca2+UniRule annotationNote: Binds two Mg(2+) per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi435 – 4351Magnesium 1; catalyticUniRule annotation
Sitei460 – 4601Interaction with DNAUniRule annotation
Sitei463 – 4631Interaction with DNAUniRule annotation
Metal bindingi508 – 5081Magnesium 1; catalyticUniRule annotation
Metal bindingi508 – 5081Magnesium 2UniRule annotation
Metal bindingi510 – 5101Magnesium 2UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA-dependent DNA replication Source: UniProtKB-HAMAP
  2. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO186103:GHJG-679-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrBUniRule annotation
Ordered Locus Names:spyM18_0795
OrganismiStreptococcus pyogenes serotype M18 (strain MGAS8232)
Taxonomic identifieri186103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000820: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 650650DNA gyrase subunit BPRO_0000145352Add
BLAST

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis.UniRule annotation

Protein-protein interaction databases

STRINGi186103.spyM18_0795.

Structurei

3D structure databases

ProteinModelPortaliQ8P1M4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini429 – 543115ToprimUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type II topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0187.
HOGENOMiHOG000075154.
KOiK02470.
OMAiHIIIMTD.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_01898. GyrB.
InterProiIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01059. gyrB. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8P1M4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIEENKHFEK KMQEYDASQI QVLEGLEAVR MRPGMYIGST AKEGLHHLVW
60 70 80 90 100
EIVDNSIDEA LAGFASHIKV FIEADNSITV VDDGRGIPVD IQAKTGRPAV
110 120 130 140 150
ETVFTVLHAG GKFGGGGYKV SGGLHGVGSS VVNALSTQLD VRVYKNGQIH
160 170 180 190 200
YQEFKRGAVV ADLEIIGTTD VTGTTVHFTP DPEIFTETTQ FDYSVLAKRI
210 220 230 240 250
QELAFLNRGL KISITDKRSG MEQEEHFHYE GGIGSYVEFL NDKKDVIFET
260 270 280 290 300
PIYTDGELEG IAVEVAMQYT TSYQETVMSF ANNIHTHEGG THEQGFRAAL
310 320 330 340 350
TRVINDYAKK NKILKENEDN LTGEDVREGL TAVISVKHPN PQFEGQTKTK
360 370 380 390 400
LGNSEVVKIT NRLFSEAFQR FLLENPQVAR KIVEKGILAS KARIAAKRAR
410 420 430 440 450
EVTRKKSGLE ISNLPGKLAD CSSNDANQNE LFIVEGDSAG GSAKSGRNRE
460 470 480 490 500
FQAILPIRGK ILNVEKATMD KILANEEIRS LFTAMGTGFG ADFDVSKARY
510 520 530 540 550
QKLVIMTDAD VDGAHIRTLL LTLIYRFMRP VLEAGYVYIA QPPIYGVKVG
560 570 580 590 600
SEIKEYIQPG IDQEDQLKTA LEKYSIGRSK PTVQRYKGLG EMDDHQLWET
610 620 630 640 650
TMDPENRLMA RVTVDDAAEA DKVFDMLMGD RVEPRRDFIE ENAVYSTLDI
Length:650
Mass (Da):72,309
Last modified:October 1, 2002 - v1
Checksum:i2CF598897B872ACB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009949 Genomic DNA. Translation: AAL97459.1.
RefSeqiNP_606960.1. NC_003485.1.

Genome annotation databases

EnsemblBacteriaiAAL97459; AAL97459; spyM18_0795.
GeneIDi994970.
KEGGispm:spyM18_0795.
PATRICi19748210. VBIStrPyo4396_0695.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009949 Genomic DNA. Translation: AAL97459.1 .
RefSeqi NP_606960.1. NC_003485.1.

3D structure databases

ProteinModelPortali Q8P1M4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186103.spyM18_0795.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL97459 ; AAL97459 ; spyM18_0795 .
GeneIDi 994970.
KEGGi spm:spyM18_0795.
PATRICi 19748210. VBIStrPyo4396_0695.

Phylogenomic databases

eggNOGi COG0187.
HOGENOMi HOG000075154.
KOi K02470.
OMAi HIIIMTD.
OrthoDBi EOG6P334W.

Enzyme and pathway databases

BioCyci SPYO186103:GHJG-679-MONOMER.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPi MF_01898. GyrB.
InterProi IPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsi TIGR01059. gyrB. 1 hit.
PROSITEi PS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks."
    Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS8232.

Entry informationi

Entry nameiGYRB_STRP8
AccessioniPrimary (citable) accession number: Q8P1M4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3