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Q8P1M4

- GYRB_STRP8

UniProt

Q8P1M4 - GYRB_STRP8

Protein

DNA gyrase subunit B

Gene

gyrB

Organism
Streptococcus pyogenes serotype M18 (strain MGAS8232)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.UniRule annotation

    Catalytic activityi

    ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi435 – 4351Magnesium 1; catalyticUniRule annotation
    Sitei460 – 4601Interaction with DNAUniRule annotation
    Sitei463 – 4631Interaction with DNAUniRule annotation
    Metal bindingi508 – 5081Magnesium 1; catalyticUniRule annotation
    Metal bindingi508 – 5081Magnesium 2UniRule annotation
    Metal bindingi510 – 5101Magnesium 2UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. DNA binding Source: UniProtKB-HAMAP
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA-dependent DNA replication Source: UniProtKB-HAMAP
    2. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    ATP-binding, DNA-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSPYO186103:GHJG-679-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA gyrase subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
    Gene namesi
    Name:gyrBUniRule annotation
    Ordered Locus Names:spyM18_0795
    OrganismiStreptococcus pyogenes serotype M18 (strain MGAS8232)
    Taxonomic identifieri186103 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000000820: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 650650DNA gyrase subunit BPRO_0000145352Add
    BLAST

    Interactioni

    Subunit structurei

    Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis.UniRule annotation

    Protein-protein interaction databases

    STRINGi186103.spyM18_0795.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8P1M4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini429 – 543115ToprimUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type II topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0187.
    HOGENOMiHOG000075154.
    KOiK02470.
    OMAiHIIIMTD.
    OrthoDBiEOG6P334W.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    3.40.50.670. 1 hit.
    HAMAPiMF_01898. GyrB.
    InterProiIPR002288. DNA_gyrase_B_C.
    IPR011557. GyrB.
    IPR003594. HATPase_ATP-bd.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR001241. Topo_IIA.
    IPR013506. Topo_IIA_bsu_dom2.
    IPR013759. Topo_IIA_cen_dom.
    IPR013760. Topo_IIA_like_dom.
    IPR018522. TopoIIA_CS.
    IPR006171. Toprim_domain.
    [Graphical view]
    PfamiPF00204. DNA_gyraseB. 1 hit.
    PF00986. DNA_gyraseB_C. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00418. TPI2FAMILY.
    SMARTiSM00387. HATPase_c. 1 hit.
    SM00433. TOP2c. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    SSF56719. SSF56719. 1 hit.
    TIGRFAMsiTIGR01059. gyrB. 1 hit.
    PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8P1M4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIEENKHFEK KMQEYDASQI QVLEGLEAVR MRPGMYIGST AKEGLHHLVW    50
    EIVDNSIDEA LAGFASHIKV FIEADNSITV VDDGRGIPVD IQAKTGRPAV 100
    ETVFTVLHAG GKFGGGGYKV SGGLHGVGSS VVNALSTQLD VRVYKNGQIH 150
    YQEFKRGAVV ADLEIIGTTD VTGTTVHFTP DPEIFTETTQ FDYSVLAKRI 200
    QELAFLNRGL KISITDKRSG MEQEEHFHYE GGIGSYVEFL NDKKDVIFET 250
    PIYTDGELEG IAVEVAMQYT TSYQETVMSF ANNIHTHEGG THEQGFRAAL 300
    TRVINDYAKK NKILKENEDN LTGEDVREGL TAVISVKHPN PQFEGQTKTK 350
    LGNSEVVKIT NRLFSEAFQR FLLENPQVAR KIVEKGILAS KARIAAKRAR 400
    EVTRKKSGLE ISNLPGKLAD CSSNDANQNE LFIVEGDSAG GSAKSGRNRE 450
    FQAILPIRGK ILNVEKATMD KILANEEIRS LFTAMGTGFG ADFDVSKARY 500
    QKLVIMTDAD VDGAHIRTLL LTLIYRFMRP VLEAGYVYIA QPPIYGVKVG 550
    SEIKEYIQPG IDQEDQLKTA LEKYSIGRSK PTVQRYKGLG EMDDHQLWET 600
    TMDPENRLMA RVTVDDAAEA DKVFDMLMGD RVEPRRDFIE ENAVYSTLDI 650
    Length:650
    Mass (Da):72,309
    Last modified:October 1, 2002 - v1
    Checksum:i2CF598897B872ACB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009949 Genomic DNA. Translation: AAL97459.1.
    RefSeqiNP_606960.1. NC_003485.1.

    Genome annotation databases

    EnsemblBacteriaiAAL97459; AAL97459; spyM18_0795.
    GeneIDi994970.
    KEGGispm:spyM18_0795.
    PATRICi19748210. VBIStrPyo4396_0695.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE009949 Genomic DNA. Translation: AAL97459.1 .
    RefSeqi NP_606960.1. NC_003485.1.

    3D structure databases

    ProteinModelPortali Q8P1M4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 186103.spyM18_0795.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL97459 ; AAL97459 ; spyM18_0795 .
    GeneIDi 994970.
    KEGGi spm:spyM18_0795.
    PATRICi 19748210. VBIStrPyo4396_0695.

    Phylogenomic databases

    eggNOGi COG0187.
    HOGENOMi HOG000075154.
    KOi K02470.
    OMAi HIIIMTD.
    OrthoDBi EOG6P334W.

    Enzyme and pathway databases

    BioCyci SPYO186103:GHJG-679-MONOMER.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    3.40.50.670. 1 hit.
    HAMAPi MF_01898. GyrB.
    InterProi IPR002288. DNA_gyrase_B_C.
    IPR011557. GyrB.
    IPR003594. HATPase_ATP-bd.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    IPR001241. Topo_IIA.
    IPR013506. Topo_IIA_bsu_dom2.
    IPR013759. Topo_IIA_cen_dom.
    IPR013760. Topo_IIA_like_dom.
    IPR018522. TopoIIA_CS.
    IPR006171. Toprim_domain.
    [Graphical view ]
    Pfami PF00204. DNA_gyraseB. 1 hit.
    PF00986. DNA_gyraseB_C. 1 hit.
    PF02518. HATPase_c. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00418. TPI2FAMILY.
    SMARTi SM00387. HATPase_c. 1 hit.
    SM00433. TOP2c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    SSF56719. SSF56719. 1 hit.
    TIGRFAMsi TIGR01059. gyrB. 1 hit.
    PROSITEi PS00177. TOPOISOMERASE_II. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks."
      Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M.
      Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MGAS8232.

    Entry informationi

    Entry nameiGYRB_STRP8
    AccessioniPrimary (citable) accession number: Q8P1M4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3