Reviewed, UniProtKB/Swiss-Prot Q8P1F1 (PANE_STRP8)

Names and origin

Protein names

Recommended name:
    Putative 2-dehydropantoate 2-reductase
    EC=1.1.1.169
Alternative name(s):
    Ketopantoate reductase
      Short name=KPA reductase
      Short name=KPR

Gene names

Name:	apbA
Ordered Locus Names:	spyM18_0911

Organism

Streptococcus pyogenes serotype M18 [Complete proteome] [HAMAP]

Taxonomic identifier

301451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Putative 2-dehydropantoate 2-reductase		PRO_0000157321
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	184	1	Proton donor By similarity
	Binding site	102	1	NADP; via amide nitrogen By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	188	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
	Binding site	268	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8P1F1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: AC4DCD25E5A1CE96	FASTA	307	33,841
	10 20 30 40 50 60 MLVYIAGSGA MGCRFGYQIS KTNNDVILLD NWEDHINAIK ENGLVVTGDV EETVKLPIMK 70 80 90 100 110 120 PTEATQEADL IILFTKAMQL PQMLQDIKGI IGKETKVLCL LNGLGHEDVI RQYIPEHNIL 130 140 150 160 170 180 MGVTVWTAGL EGPGRAHLQG VGALNLQSMD PNNQDAGHQV ADLLNKANLN ATYDENVVPN 190 200 210 220 230 240 IWRKACVNGT MNSTCALLDC TIGELFASED GLKMVKEIIH EFVIVGQAEG VELNEEEITQ 250 260 270 280 290 300 YVMDTSVKAA HHYPSMHQDL VQNHRLTEID FINGAVNTKG EKLGINTPYC RMITELVHAK EAVLNIQ

References

[1]

"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks." Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed: 11917108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MGAS8232 / Serotype M18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009949 Genomic DNA. Translation: AAL97561.1.
RefSeq	NP_607062.1.
3D structure databases
SMR	Q8P1F1. Positions 1-304.
ModBase	Search...
Genome annotation databases
GeneID	994053.
GenomeReviews	Gene locus spyM18_0911 in contig AE009949_GR.
KEGG	spm:spyM18_0911.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG668370.
OMA	IDSWQEH.
Enzyme and pathway databases
BioCyc	SPYO186103:SPYM18_0911-MONOMER.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21708:SF21. ApbA. 1 hit.
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_STRP8

Accession

Primary (citable) accession number: Q8P1F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	October 1, 2002
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)