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Q8P068

- SYI_STRP8

UniProt

Q8P068 - SYI_STRP8

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptococcus pyogenes serotype M18 (strain MGAS8232)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO186103:GHJG-1319-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:spyM18_1531
OrganismiStreptococcus pyogenes serotype M18 (strain MGAS8232)
Taxonomic identifieri186103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000820: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 933933Isoleucine--tRNA ligasePRO_0000098482Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi186103.spyM18_1531.

Structurei

3D structure databases

ProteinModelPortaliQ8P068.
SMRiQ8P068. Positions 1-918.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8P068-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLKETLNLG KTAFPMRADL PNKEPQWQAA WEQAELYKKR QELNAGKPAF
60 70 80 90 100
HLHDGPPYAN GNIHVGHALN KISKDIIVRS KSMSGFQAPY VPGWDTHGLP
110 120 130 140 150
IEQVLAKQGI KRKEMDLAEY LEMCRQYALS QVDKQRDDFK RLGVSADWEN
160 170 180 190 200
PYVTLDPQFE ADQIRVFGAM AEKGYIYRGA KPVYWSWSSE SALAEAEIEY
210 220 230 240 250
HDIDSTSLYY ANKVKDGKGI LDTNTYIVVW TTTPFTVTAS RGLTVGPDMD
260 270 280 290 300
YLVVKPAGSD RQYVVAEGLL DSLAGKFGWE SFETLASHKG ADLEYIVTEH
310 320 330 340 350
PWDTDVEELV ILGDHVTLES GTGIVHTAPG FGEDDYNVGT KYKLEVAVTV
360 370 380 390 400
DERGLMMENA GPDFHGQFYN KVTPIVIDKL GDLLLAQEVI NHSYPFDWRT
410 420 430 440 450
KKPIIWRAVP QWFASVSDFR QDILDEIEKT TFHPSWGETR LYNMIRDRGD
460 470 480 490 500
WVISRQRAWG VPLSIFYAED GTAIMTKEVT DHVADLFQEN GSIIWWQKEA
510 520 530 540 550
KDLLPEGFTH PGSPNGEFTK ETDIMDVWFD SGSSWNGVMN ARENLSYPAD
560 570 580 590 600
LYLEGSDQYR GWFNSSLITS VAVNGHAPYK AILSQGFVLD GKGEKMSKSK
610 620 630 640 650
GNIISPNDVA KQYGADILRL WVASVDTDND VRVSMEILGQ VSETYRKIRN
660 670 680 690 700
TLRFLIANTS DFNPATDTVA YADLGAVDKY MTIVFNQLVA TITDAYERYD
710 720 730 740 750
FMAIYKAVVN FVTVDLSAFY LDFAKDVVYI EAANSLERRR MQTVFYDILV
760 770 780 790 800
KITKLLTPIL PHTTEEIWSY LEYESEAFVQ LAEMPVAETF SAQEDILEAW
810 820 830 840 850
SAFMTLRTQA QKALEEARNA KIIGKSLEAH LTIYASEEVK TLLTALDSDI
860 870 880 890 900
ALLLIVSQLT IADLADAPAD AVAFEGIAFM VEHAIGEVCE RSRRIDPTTR
910 920 930
MRSYNAFVCD HSAKIIEENF PEAVAEGFEE SGK
Length:933
Mass (Da):105,113
Last modified:October 1, 2002 - v1
Checksum:iC93E9EBD10AEAF28
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009949 Genomic DNA. Translation: AAL98099.1.
RefSeqiNP_607600.1. NC_003485.1.

Genome annotation databases

EnsemblBacteriaiAAL98099; AAL98099; spyM18_1531.
GeneIDi994701.
KEGGispm:spyM18_1531.
PATRICi19749576. VBIStrPyo4396_1374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE009949 Genomic DNA. Translation: AAL98099.1 .
RefSeqi NP_607600.1. NC_003485.1.

3D structure databases

ProteinModelPortali Q8P068.
SMRi Q8P068. Positions 1-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186103.spyM18_1531.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL98099 ; AAL98099 ; spyM18_1531 .
GeneIDi 994701.
KEGGi spm:spyM18_1531.
PATRICi 19749576. VBIStrPyo4396_1374.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPYO186103:GHJG-1319-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks."
    Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS8232.

Entry informationi

Entry nameiSYI_STRP8
AccessioniPrimary (citable) accession number: Q8P068
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3