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Q8NZU4

- TRMB_STRP8

UniProt

Q8NZU4 - TRMB_STRP8

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Streptococcus pyogenes serotype M18 (strain MGAS8232)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441S-adenosyl-L-methionineUniRule annotation
Binding sitei69 – 691S-adenosyl-L-methionineUniRule annotation
Binding sitei96 – 961S-adenosyl-L-methionineUniRule annotation
Active sitei118 – 1181By similarity
Binding sitei118 – 1181S-adenosyl-L-methionineUniRule annotation
Binding sitei122 – 1221SubstrateUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSPYO186103:GHJG-1494-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:spyM18_1734
OrganismiStreptococcus pyogenes serotype M18 (strain MGAS8232)
Taxonomic identifieri186103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000820: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211tRNA (guanine-N(7)-)-methyltransferasePRO_0000171408Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi186103.spyM18_1734.

Structurei

3D structure databases

ProteinModelPortaliQ8NZU4.
SMRiQ8NZU4. Positions 6-211.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1296Interaction with RNAUniRule annotation
Regioni191 – 1944Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NZU4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRVRKRKGAE KHLANNPHYV ILNPEDAKGR WHDVFGNDRP IHIEVGSGKG
60 70 80 90 100
GFITGMALKN PDINYIGIDI QLSVLSYALD KVLASEVPNV KLLRVDGSSL
110 120 130 140 150
TNYFEDGEVD MMYLNFSDPW PKTKHEKRRL TYKDFLDTYK RILPEHGEIH
160 170 180 190 200
FKTDNRGLFE YSLASFSQYG MTLRQIWLDL HASNYEGNVM TEYEEKFSNK
210
GQVIYRVEAN F
Length:211
Mass (Da):24,454
Last modified:October 1, 2002 - v1
Checksum:iFED21DCC8B393860
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009949 Genomic DNA. Translation: AAL98263.1.
RefSeqiNP_607764.1. NC_003485.1.
WP_011018101.1. NC_003485.1.

Genome annotation databases

EnsemblBacteriaiAAL98263; AAL98263; spyM18_1734.
GeneIDi994557.
KEGGispm:spyM18_1734.
PATRICi19749956. VBIStrPyo4396_1551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009949 Genomic DNA. Translation: AAL98263.1 .
RefSeqi NP_607764.1. NC_003485.1.
WP_011018101.1. NC_003485.1.

3D structure databases

ProteinModelPortali Q8NZU4.
SMRi Q8NZU4. Positions 6-211.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186103.spyM18_1734.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL98263 ; AAL98263 ; spyM18_1734 .
GeneIDi 994557.
KEGGi spm:spyM18_1734.
PATRICi 19749956. VBIStrPyo4396_1551.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci SPYO186103:GHJG-1494-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks."
    Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS8232.

Entry informationi

Entry nameiTRMB_STRP8
AccessioniPrimary (citable) accession number: Q8NZU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: October 1, 2002
Last modified: November 26, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3