Reviewed, UniProtKB/Swiss-Prot Q8NZ49 (FTHS2_STRP8)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase 2
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase 2
      Short name=FHS 2
      Short name=FTHFS 2

Gene names

Name:	fhs2
Ordered Locus Names:	spyM18_2144

Organism

Streptococcus pyogenes serotype M18 [Complete proteome] [HAMAP]

Taxonomic identifier

301451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Formate--tetrahydrofolate ligase 2 HAMAP MF_01543		PRO_0000199392
Regions
	Nucleotide binding	66 – 73	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NZ49-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 7A7222A02BD5A804	FASTA	557	59,054
	10 20 30 40 50 60 MVLSDIEIAN SVTMEPISKV ANQLGIDEEA LCLYGKYKAK IDARQLVALK DKPDGKLILV 70 80 90 100 110 120 TAISPTPAGE GKTTTSVGLV DALSAIGKKA VIALREPSLG PVFGVKGGAA GGGHAQVVPM 130 140 150 160 170 180 EDINLHFTGD FHAIGVANNL LAALIDNHIH HGNSLGIDSR RITWKRVVDM NDRQLRHIVD 190 200 210 220 230 240 GLQGKVNGVP REDGYDITVA SEIMAILCLS ENISDLKARL EKIIIGYNYQ GEPVTAKDLK 250 260 270 280 290 300 AGGALAALLK DAIHPNLVQT LEHTPALIHG GPFANIAHGC NSVLATKLAL KYGDYAVTEA 310 320 330 340 350 360 GFGADLGAEK FIDIKCRMSG LRPAAVVLVA TIRALKMHGG VPKADLATEN VQAVVDGLPN 370 380 390 400 410 420 LDKHLANIQD VYGLPVVVAI NKFPLDTDAE LQAVYDACDK RGVDVVISDV WANGGAGGRE 430 440 450 460 470 480 LAEKVVALAE QDNQFCFVYE EDDSIETKLT KIVTKVYGGK GIRLTPAAKR ELADLERLSF 490 500 510 520 530 540 GNYPICMAKT QYSFSDDAKK LGAPTDFTVT ISNLKVSAGA GFIVALTGAI MTMPGLPKVP 550 ASETIDIDEE GNITGLF

References

[1]

"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks." Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed: 11917108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MGAS8232 / Serotype M18.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE009949 Genomic DNA. Translation: AAL98593.1.
RefSeq	NP_608094.1.
3D structure databases
HSSP	HSSP built from PDB template 1EG7 based on UniProtKB P21164.
ModBase	Search...
Genome annotation databases
GeneID	994232.
GenomeReviews	Gene locus spyM18_2144 in contig AE009949_GR.
KEGG	spm:spyM18_2144.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NZ49.
OMA	KMPGLPK.
Enzyme and pathway databases
BioCyc	SPYO186103:SPYM18_2144-MON.
Family and domain databases
HAMAP	MF_01543. [Tree]
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS2_STRP8

Accession

Primary (citable) accession number: Q8NZ49

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)