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Protein

Formate--tetrahydrofolate ligase 2

Gene

fhs2

Organism
Streptococcus pyogenes serotype M18 (strain MGAS8232)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 738ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPYO186103:GHJG-1833-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase 2UniRule annotation (EC:6.3.4.3UniRule annotation)
Alternative name(s):
Formyltetrahydrofolate synthetase 2UniRule annotation
Short name:
FHS 2UniRule annotation
Short name:
FTHFS 2UniRule annotation
Gene namesi
Name:fhs2UniRule annotation
Ordered Locus Names:spyM18_2144
OrganismiStreptococcus pyogenes serotype M18 (strain MGAS8232)
Taxonomic identifieri186103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000820: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Formate--tetrahydrofolate ligase 2PRO_0000199392Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi186103.spyM18_2144.

Structurei

3D structure databases

ProteinModelPortaliQ8NZ49.
SMRiQ8NZ49. Positions 5-555.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the formate--tetrahydrofolate ligase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiMDMNDRQ.
OrthoDBiEOG6PCPSP.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NZ49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSDIEIAN SVTMEPISKV ANQLGIDEEA LCLYGKYKAK IDARQLVALK
60 70 80 90 100
DKPDGKLILV TAISPTPAGE GKTTTSVGLV DALSAIGKKA VIALREPSLG
110 120 130 140 150
PVFGVKGGAA GGGHAQVVPM EDINLHFTGD FHAIGVANNL LAALIDNHIH
160 170 180 190 200
HGNSLGIDSR RITWKRVVDM NDRQLRHIVD GLQGKVNGVP REDGYDITVA
210 220 230 240 250
SEIMAILCLS ENISDLKARL EKIIIGYNYQ GEPVTAKDLK AGGALAALLK
260 270 280 290 300
DAIHPNLVQT LEHTPALIHG GPFANIAHGC NSVLATKLAL KYGDYAVTEA
310 320 330 340 350
GFGADLGAEK FIDIKCRMSG LRPAAVVLVA TIRALKMHGG VPKADLATEN
360 370 380 390 400
VQAVVDGLPN LDKHLANIQD VYGLPVVVAI NKFPLDTDAE LQAVYDACDK
410 420 430 440 450
RGVDVVISDV WANGGAGGRE LAEKVVALAE QDNQFCFVYE EDDSIETKLT
460 470 480 490 500
KIVTKVYGGK GIRLTPAAKR ELADLERLSF GNYPICMAKT QYSFSDDAKK
510 520 530 540 550
LGAPTDFTVT ISNLKVSAGA GFIVALTGAI MTMPGLPKVP ASETIDIDEE

GNITGLF
Length:557
Mass (Da):59,054
Last modified:October 1, 2002 - v1
Checksum:i7A7222A02BD5A804
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009949 Genomic DNA. Translation: AAL98593.1.
RefSeqiNP_608094.1. NC_003485.1.

Genome annotation databases

EnsemblBacteriaiAAL98593; AAL98593; spyM18_2144.
GeneIDi994232.
KEGGispm:spyM18_2144.
PATRICi19750694. VBIStrPyo4396_1911.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009949 Genomic DNA. Translation: AAL98593.1.
RefSeqiNP_608094.1. NC_003485.1.

3D structure databases

ProteinModelPortaliQ8NZ49.
SMRiQ8NZ49. Positions 5-555.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186103.spyM18_2144.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL98593; AAL98593; spyM18_2144.
GeneIDi994232.
KEGGispm:spyM18_2144.
PATRICi19750694. VBIStrPyo4396_1911.

Phylogenomic databases

eggNOGiCOG2759.
HOGENOMiHOG000040280.
KOiK01938.
OMAiMDMNDRQ.
OrthoDBiEOG6PCPSP.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciSPYO186103:GHJG-1833-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks."
    Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M.
    Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MGAS8232.

Entry informationi

Entry nameiFTHS2_STRP8
AccessioniPrimary (citable) accession number: Q8NZ49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: October 1, 2002
Last modified: February 4, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.