Reviewed, UniProtKB/Swiss-Prot Q8NYG1 (LYTM_STAAW)

Names and origin

Protein names

Recommended name:
    Glycyl-glycine endopeptidase lytM
    EC=3.4.24.75
Alternative name(s):
    Autolysin lytM

Gene names

Name:	lytM
Ordered Locus Names:	MW0252

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Peptidoglycan hydrolase (autolysin) specifically acting on polyglycine interpeptide bridges of the cell wall peptidoglycan By similarity.
Catalytic activity	Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the peptidase M23B family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation Virulence
Cellular component	Secreted
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	By similarity
	Chain	26 – 316	291	Glycyl-glycine endopeptidase lytM		PRO_0000026825
Sites
	Metal binding	117	1	Zinc By similarity
	Metal binding	210	1	Zinc By similarity
	Metal binding	214	1	Zinc By similarity
	Metal binding	293	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NYG1-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 3A53C4331E8B024F	FASTA	316	34,355
	10 20 30 40 50 60 MKKLTAAAIA TMGFATFTMA HQADAAETTN TQQAHTQMST QSQDVSYGTY YTIDSNGDYH 70 80 90 100 110 120 HTPDGNWNQA MFDNKEYSYT FVDAQGHTHY FYNCYPKNAN ANGSGQTYVN PAIAGDNNDY 130 140 150 160 170 180 TASQSQQHIN QYGYQSNVGP DASYYSHSNN NQAYNSHDGN GKVNYPNGTS NQNGGLASKA 190 200 210 220 230 240 TASGHAKDAS WLTSRKQLQP YGQYHGGGAH YGVDYAMPEN SPVYSLTDGT VVQAGWSNYG 250 260 270 280 290 300 GGNQVTIKEA NSNNYQWYMH NNRLTVSAGD KVKAGDQIAY SGSTGNSTAP HVHFQRMSGG 310 IGNQYAVDPT SYLQSR

References

[1]

"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed: 12044378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000033 Genomic DNA. Translation: BAB94117.1.
RefSeq	NP_645067.1.
3D structure databases
SMR	Q8NYG1. Positions 45-314, 185-315.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8NYG1.
Protein family/group databases
MEROPS	M23.004.
Genome annotation databases
GeneID	1002352.
GenomeReviews	Gene locus MW0252 in contig BA000033_GR.
KEGG	sam:MW0252.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3942.
HOGENOM	HBG693570.
OMA	AYNSHNG.
Enzyme and pathway databases
BioCyc	SAUR196620:MW0252-MONOMER.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR016047. Peptidase_M23. IPR002886. Peptidase_M23B. [Graphical view]
PANTHER	PTHR21666:SF7. Peptidase_M23B. 1 hit.
Pfam	PF01551. Peptidase_M23. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYTM_STAAW

Accession

Primary (citable) accession number: Q8NYG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	October 1, 2002
Last modified:	January 19, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)