Q8NY00 (SYM_STAAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Synonyms:	metS
Ordered Locus Names:	MW0445

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	657 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 657	657	Methionine--tRNA ligase HAMAP-Rule MF_01228		PRO_0000139244
Regions
	Domain	557 – 657	101	tRNA-binding
	Motif	13 – 23	11	"HIGH" region HAMAP-Rule MF_01228
	Motif	308 – 312	5	"KMSKS" region HAMAP-Rule MF_01228
Sites
	Binding site	311	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NY00 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 12C96E35EFB3D01A	FASTA	657	74,872
	10 20 30 40 50 60 MAKETFYITT PIYYPSGNLH IGHAYSTVAG DVIARYKRMQ GYDVRYLTGT DEHGQKIQEK 70 80 90 100 110 120 AQKAGKTEIE YLDEMIAGIK QLWAKLEISN DDFIRTTEER HKHVVEQVFE RLLKQGDIYL 130 140 150 160 170 180 GEYEGWYSVP DETYYTESQL VDPQYENGKI IGGKSPDSGH EVELVKEESY FFNISKYTDR 190 200 210 220 230 240 LLEFYDQNPD FIQPPSRKNE MINNFIKPGL ADLAVSRTSF NWGVHVPSNP KHVVYVWIDA 250 260 270 280 290 300 LVNYISALGY LSDDESLFNK YWPADIHLMA KEIVRFHSII WPILLMALDL PLPKKVFAHG 310 320 330 340 350 360 WILMKDGKMS KSKGNVVDPN ILIDRYGLDA TRYYLMRELP FGSDGVFTPE AFVERTNFDL 370 380 390 400 410 420 ANDLGNLVNR TISMVNKYFD GELPAYQGPL HELDEEMEAM ALETVKSYTE SMESLQFSVA 430 440 450 460 470 480 LSTVWKFISR TNKYIDETTP WVLAKDDSQK DMLGNVMAHL VENIRYAAVL LRPFLTHAPK 490 500 510 520 530 540 EIFEQLNINN PQFMEFSSLE QYGVLTESIM VTGQPKPIFP RLDSEAEIAY IKESMQPPAT 550 560 570 580 590 600 EEEKEEIPSK PQIDIKDFDK VEIKAATIIN AEHVKKSDKL LKIQVDLDSE QRQIVSGIAK 610 620 630 640 650 FYTPDDIIGK KVAVVTNLKP AKLMGQKSEG MILSAEKDGV LTLVSLPSAI PNGAVIK

References

[1]

"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MW2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000033 Genomic DNA. Translation: BAB94310.1.
RefSeq	NP_645262.1. NC_003923.1.
3D structure databases
ProteinModelPortal	Q8NY00.
ModBase	Search...
Protein-protein interaction databases
STRING	196620.MW0445.
Proteomic databases
PRIDE	Q8NY00.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB94310; BAB94310; BAB94310.
GeneID	1002549.
KEGG	sam:MW0445.
PATRIC	19567388. VBIStaAur44266_0472.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0143.
HOGENOM	HOG000200401.
KO	K01874.
OMA	RINYDLA.
ProtClustDB	PRK12267.
Enzyme and pathway databases
BioCyc	SAUR196620:GJ9Z-450-MONOMER.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.40.50.620. 2 hits.
HAMAP	MF_01228. Met_tRNA_synth_type2.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR014758. Met-tRNA_synth. IPR023457. Met-tRNA_synth_2. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	Q8NY00.
ChEMBL	CHEMBL5372.

Entry information

Entry name

SYM_STAAW

Accession

Primary (citable) accession number: Q8NY00

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program