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Protein

Thermonuclease

Gene

nuc

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100CalciumPROSITE-ProRule annotation1
Active sitei114By similarity1
Metal bindingi119CalciumPROSITE-ProRule annotation1
Metal bindingi120Calcium; via carbonyl oxygenPROSITE-ProRule annotation1
Active sitei122By similarity1
Active sitei166By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thermonuclease (EC:3.1.31.1)
Short name:
TNase
Alternative name(s):
Micrococcal nuclease
Staphylococcal nuclease
Gene namesi
Name:nuc
Ordered Locus Names:MW0769
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
PropeptideiPRO_000004523724 – 60By similarityAdd BLAST37
ChainiPRO_000004523861 – 228ThermonucleaseAdd BLAST168

Keywords - PTMi

Zymogen

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi88 – 96Combined sources9
Beta strandi98 – 106Combined sources9
Beta strandi109 – 115Combined sources7
Beta strandi116 – 119Combined sources4
Beta strandi125 – 131Combined sources7
Helixi134 – 146Combined sources13
Beta strandi151 – 154Combined sources4
Beta strandi160 – 162Combined sources3
Turni163 – 165Combined sources3
Beta strandi167 – 173Combined sources7
Helixi178 – 184Combined sources7
Beta strandi187 – 190Combined sources4
Helixi195 – 197Combined sources3
Helixi201 – 213Combined sources13
Helixi217 – 219Combined sources3
Turni221 – 223Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LKVNMR-A80-228[»]
2M00NMR-A80-228[»]
2OXPX-ray2.00A80-228[»]
2PW5X-ray2.10A80-228[»]
2PW7X-ray2.10A80-228[»]
2PYKX-ray2.10A80-228[»]
2PZTX-ray2.10A80-228[»]
2PZUX-ray2.10A80-228[»]
2PZWX-ray2.10A80-228[»]
3D4WX-ray1.90A80-228[»]
3D8GX-ray1.99A80-228[»]
3MVVX-ray1.72A80-228[»]
3QOJX-ray1.60A80-228[»]
3QOLX-ray1.90A80-228[»]
3QONX-ray2.00A80-228[»]
3R3OX-ray1.90A80-228[»]
ProteinModelPortaliQ8NXI6.
SMRiQ8NXI6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NXI6.

Family & Domainsi

Sequence similaritiesi

Belongs to the thermonuclease family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000017398.
KOiK01174.
OMAiNEMTFIG.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NXI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYLLSAGI CMAIVSILLI GMAISNVSKG QYAKRFFFFA TSCLVLTLVV
60 70 80 90 100
VSSLSSSANA SQTDNGVNRS GSEHPTVYSA TSTKKLHKEP ATLIKAIDGD
110 120 130 140 150
TVKLMYKGQP MTFRLLLVDT PETKHPKKGV EKYGPEASAF TKKMVENAKK
160 170 180 190 200
IEVEFDKGQR TDKYGRGLAY IYADGKMVNE ALVRQGLAKV AYVYKPNNTH
210 220
EQLLRKSEAQ AKKEKLNIWS EDNADSGQ
Length:228
Mass (Da):25,126
Last modified:October 1, 2002 - v1
Checksum:i14DA80DB4FBD43FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94634.1.
RefSeqiWP_001793574.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB94634; BAB94634; BAB94634.
KEGGisam:MW0769.
PATRICi19568097. VBIStaAur44266_0809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94634.1.
RefSeqiWP_001793574.1. NC_003923.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LKVNMR-A80-228[»]
2M00NMR-A80-228[»]
2OXPX-ray2.00A80-228[»]
2PW5X-ray2.10A80-228[»]
2PW7X-ray2.10A80-228[»]
2PYKX-ray2.10A80-228[»]
2PZTX-ray2.10A80-228[»]
2PZUX-ray2.10A80-228[»]
2PZWX-ray2.10A80-228[»]
3D4WX-ray1.90A80-228[»]
3D8GX-ray1.99A80-228[»]
3MVVX-ray1.72A80-228[»]
3QOJX-ray1.60A80-228[»]
3QOLX-ray1.90A80-228[»]
3QONX-ray2.00A80-228[»]
3R3OX-ray1.90A80-228[»]
ProteinModelPortaliQ8NXI6.
SMRiQ8NXI6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB94634; BAB94634; BAB94634.
KEGGisam:MW0769.
PATRICi19568097. VBIStaAur44266_0809.

Phylogenomic databases

HOGENOMiHOG000017398.
KOiK01174.
OMAiNEMTFIG.

Miscellaneous databases

EvolutionaryTraceiQ8NXI6.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUC_STAAW
AccessioniPrimary (citable) accession number: Q8NXI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.