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Protein

Thermonuclease

Gene

nuc

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5' position of the phosphodiester bond.By similarity

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi100 – 1001CalciumPROSITE-ProRule annotation
Active sitei114 – 1141By similarity
Metal bindingi119 – 1191CalciumPROSITE-ProRule annotation
Metal bindingi120 – 1201Calcium; via carbonyl oxygenPROSITE-ProRule annotation
Active sitei122 – 1221By similarity
Active sitei166 – 1661By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-791-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermonuclease (EC:3.1.31.1)
Short name:
TNase
Alternative name(s):
Micrococcal nuclease
Staphylococcal nuclease
Gene namesi
Name:nuc
Ordered Locus Names:MW0769
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Propeptidei24 – 6037By similarityPRO_0000045237Add
BLAST
Chaini61 – 228168ThermonucleasePRO_0000045238Add
BLAST

Keywords - PTMi

Zymogen

Structurei

Secondary structure

1
228
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi88 – 969Combined sources
Beta strandi98 – 1069Combined sources
Beta strandi109 – 1157Combined sources
Beta strandi116 – 1194Combined sources
Beta strandi125 – 1317Combined sources
Helixi134 – 14613Combined sources
Beta strandi151 – 1544Combined sources
Beta strandi160 – 1623Combined sources
Turni163 – 1653Combined sources
Beta strandi167 – 1737Combined sources
Helixi178 – 1847Combined sources
Beta strandi187 – 1904Combined sources
Helixi195 – 1973Combined sources
Helixi201 – 21313Combined sources
Helixi217 – 2193Combined sources
Turni221 – 2233Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LKVNMR-A80-228[»]
2M00NMR-A80-228[»]
2OXPX-ray2.00A80-228[»]
2PW5X-ray2.10A80-228[»]
2PW7X-ray2.10A80-228[»]
2PYKX-ray2.10A80-228[»]
2PZTX-ray2.10A80-228[»]
2PZUX-ray2.10A80-228[»]
2PZWX-ray2.10A80-228[»]
3D4WX-ray1.90A80-228[»]
3D8GX-ray1.99A80-228[»]
3MVVX-ray1.72A80-228[»]
3QOJX-ray1.60A80-228[»]
3QOLX-ray1.90A80-228[»]
3QONX-ray2.00A80-228[»]
3R3OX-ray1.90A80-228[»]
ProteinModelPortaliQ8NXI6.
SMRiQ8NXI6. Positions 80-228.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NXI6.

Family & Domainsi

Sequence similaritiesi

Belongs to the thermonuclease family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000017398.
OMAiNEMTFIG.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8NXI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEYLLSAGI CMAIVSILLI GMAISNVSKG QYAKRFFFFA TSCLVLTLVV
60 70 80 90 100
VSSLSSSANA SQTDNGVNRS GSEHPTVYSA TSTKKLHKEP ATLIKAIDGD
110 120 130 140 150
TVKLMYKGQP MTFRLLLVDT PETKHPKKGV EKYGPEASAF TKKMVENAKK
160 170 180 190 200
IEVEFDKGQR TDKYGRGLAY IYADGKMVNE ALVRQGLAKV AYVYKPNNTH
210 220
EQLLRKSEAQ AKKEKLNIWS EDNADSGQ
Length:228
Mass (Da):25,126
Last modified:October 1, 2002 - v1
Checksum:i14DA80DB4FBD43FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94634.1.
RefSeqiWP_001793574.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB94634; BAB94634; BAB94634.
PATRICi19568097. VBIStaAur44266_0809.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94634.1.
RefSeqiWP_001793574.1. NC_003923.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LKVNMR-A80-228[»]
2M00NMR-A80-228[»]
2OXPX-ray2.00A80-228[»]
2PW5X-ray2.10A80-228[»]
2PW7X-ray2.10A80-228[»]
2PYKX-ray2.10A80-228[»]
2PZTX-ray2.10A80-228[»]
2PZUX-ray2.10A80-228[»]
2PZWX-ray2.10A80-228[»]
3D4WX-ray1.90A80-228[»]
3D8GX-ray1.99A80-228[»]
3MVVX-ray1.72A80-228[»]
3QOJX-ray1.60A80-228[»]
3QOLX-ray1.90A80-228[»]
3QONX-ray2.00A80-228[»]
3R3OX-ray1.90A80-228[»]
ProteinModelPortaliQ8NXI6.
SMRiQ8NXI6. Positions 80-228.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB94634; BAB94634; BAB94634.
PATRICi19568097. VBIStaAur44266_0809.

Phylogenomic databases

HOGENOMiHOG000017398.
OMAiNEMTFIG.

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-791-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8NXI6.

Family and domain databases

Gene3Di2.40.50.90. 1 hit.
InterProiIPR016071. Staphylococal_nuclease_OB-fold.
IPR002071. Thermonucl_AS.
[Graphical view]
PfamiPF00565. SNase. 1 hit.
[Graphical view]
SMARTiSM00318. SNc. 1 hit.
[Graphical view]
SUPFAMiSSF50199. SSF50199. 1 hit.
PROSITEiPS01123. TNASE_1. 1 hit.
PS01284. TNASE_2. 1 hit.
PS50830. TNASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUC_STAAW
AccessioniPrimary (citable) accession number: Q8NXI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.