Q8NXG7 (2NPD_STAAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable nitronate monooxygenase
EC=1.13.12.16

Alternative name(s):
Nitroalkane oxidase

Gene names

Ordered Locus Names:

MW0803

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites By similarity.
Catalytic activity	Ethylnitronate + O2 = acetaldehyde + nitrite + other products.
Cofactor	Binds 1 FMN per subunit By similarity.
Sequence similarities	Belongs to the nitronate monooxygenase family.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	nitronate monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 355	355	Probable nitronate monooxygenase		PRO_0000360896
Regions
	Nucleotide binding	21 – 23	3	FMN By similarity
	Nucleotide binding	216 – 218	3	FMN By similarity
	Nucleotide binding	239 – 240	2	FMN By similarity
Sites
	Active site	182	1	Proton acceptor Potential
	Binding site	182	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NXG7 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: C3453C9EBBAEB37C	FASTA	355	38,582
	10 20 30 40 50 60 MWNKNRLTQM LSIEYPIIQA GMAGSTTPKL VASVSNSGGL GTIGAGYFNT QQLEDEIDYV 70 80 90 100 110 120 RQLTSNSFGV NVFVPSQQSY TSSQIENMNA WLKPYRRALH LEEPVVKITE EQQFKCHIDT 130 140 150 160 170 180 TIKKQVPVCC FTFGIPSESI IKRLKEANIK LIGTATSVDE AIANEKAGMD AIVAQGSEAG 190 200 210 220 230 240 GHRGSFLKPK NQLPMVGTIS LVPQIVDVVS IPVIAAGGIM DGRGVLASIV LGAEGVQMGT 250 260 270 280 290 300 AFLTSQDSNA SELLRDAIIN SKETDTVVTK AFSGKLARGI NNRFIEEMSQ YEGDIPDYPI 310 320 330 340 350 QNELTSSIRK AAANIGDKEL THMWSGQSPR LATTHPANTI MSNIINQINQ IMQYK

References

[1]

"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MW2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000033 Genomic DNA. Translation: BAB94668.1.
RefSeq	NP_645620.1. NC_003923.1.
3D structure databases
ProteinModelPortal	Q8NXG7.
ModBase	Search...
Protein-protein interaction databases
STRING	196620.MW0803.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB94668; BAB94668; BAB94668.
GeneID	1002915.
KEGG	sam:MW0803.
PATRIC	19568171. VBIStaAur44266_0846.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2070.
HOGENOM	HOG000123287.
KO	K00459.
OMA	YLSLWSG.
ProtClustDB	CLSK885016.
Enzyme and pathway databases
BioCyc	SAUR196620:GJ9Z-825-MONOMER.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR004136. 2Npropane_dOase. IPR013785. Aldolase_TIM. [Graphical view]
Pfam	PF03060. NMO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

2NPD_STAAW

Accession

Primary (citable) accession number: Q8NXG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program