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Q8NX62

- HDOX1_STAAW

UniProt

Q8NX62 - HDOX1_STAAW

Protein

Heme oxygenase (staphylobilin-producing) 1

Gene

isdG

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the oxo-bilirubin chromophore staphylobilin (a mixture of the linear tetrapyrroles 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin) in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.UniRule annotation

    Catalytic activityi

    Protoheme + 4 AH2 + 4 O2 = 5-oxo-delta-bilirubin + Fe2+ + CO + 4 A + 4 H2O.UniRule annotation
    Protoheme + 4 AH2 + 4 O2 = 15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71IronUniRule annotation
    Sitei67 – 671Transition state stabilizerUniRule annotation
    Metal bindingi77 – 771Iron (heme axial ligand)UniRule annotation

    GO - Molecular functioni

    1. heme binding Source: UniProtKB-HAMAP
    2. heme oxygenase (decyclizing) activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. monooxygenase activity Source: UniProtKB-KW

    GO - Biological processi

    1. heme catabolic process Source: UniProtKB-HAMAP
    2. iron assimilation Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciSAUR196620:GJ9Z-1042-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heme oxygenase (staphylobilin-producing) 1UniRule annotation (EC:1.14.99.48UniRule annotation)
    Alternative name(s):
    Heme oxygenase 1UniRule annotation
    Heme-degrading monooxygenase 1UniRule annotation
    Iron-regulated surface determinant 1UniRule annotation
    Iron-responsive surface determinant 1UniRule annotation
    Gene namesi
    Name:isdGUniRule annotation
    Ordered Locus Names:MW1018
    OrganismiStaphylococcus aureus (strain MW2)
    Taxonomic identifieri196620 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000418: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi7 – 71N → A: Loss of activity. 1 Publication
    Mutagenesisi17 – 171K → A: No effect. 1 Publication
    Mutagenesisi23 – 231F → A: No effect. 1 Publication
    Mutagenesisi38 – 381M → A: Slight loss of activity. Changes in heme binding. 1 Publication
    Mutagenesisi67 – 671W → A: Loss of activity. 1 Publication
    Mutagenesisi70 – 701S → A: No effect. 1 Publication
    Mutagenesisi77 – 771H → A: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 107107Heme oxygenase (staphylobilin-producing) 1PRO_0000270089Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 PublicationUniRule annotation

    Protein-protein interaction databases

    STRINGi196620.MW1018.

    Structurei

    Secondary structure

    1
    107
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 1210
    Helixi16 – 216
    Helixi29 – 313
    Beta strandi35 – 4511
    Beta strandi47 – 6014
    Helixi61 – 688
    Helixi71 – 799
    Beta strandi91 – 10616

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1XBWX-ray1.90A/B/C/D1-107[»]
    ProteinModelPortaliQ8NX62.
    SMRiQ8NX62. Positions 1-107.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8NX62.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 9290ABMAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 298Heme bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.UniRule annotation
    Contains 1 ABM domain.Curated

    Phylogenomic databases

    eggNOGiCOG2329.
    HOGENOMiHOG000008026.
    KOiK07145.
    OMAiVRHQSQD.
    OrthoDBiEOG6GTZMS.

    Family and domain databases

    HAMAPiMF_01272. Heme_degrading_monooxygenase.
    InterProiIPR007138. ABM-like.
    IPR011008. Dimeric_a/b-barrel.
    IPR023953. IsdG.
    [Graphical view]
    PfamiPF03992. ABM. 1 hit.
    [Graphical view]
    SUPFAMiSSF54909. SSF54909. 1 hit.
    PROSITEiPS51725. ABM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8NX62-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKFMAENRLT LTKGTAKDII ERFYTRHGIE TLEGFDGMFV TQTLEQEDFD    50
    EVKILTVWKS KQAFTDWLKS DVFKAAHKHV RSKNEDESSP IINNKVITYD 100
    IGYSYMK 107
    Length:107
    Mass (Da):12,546
    Last modified:October 1, 2002 - v1
    Checksum:iDB13A134D5EC4FF0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000033 Genomic DNA. Translation: BAB94883.1.
    RefSeqiNP_645835.1. NC_003923.1.

    Genome annotation databases

    EnsemblBacteriaiBAB94883; BAB94883; BAB94883.
    GeneIDi1003130.
    KEGGisam:MW1018.
    PATRICi19568616. VBIStaAur44266_1067.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000033 Genomic DNA. Translation: BAB94883.1 .
    RefSeqi NP_645835.1. NC_003923.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1XBW X-ray 1.90 A/B/C/D 1-107 [» ]
    ProteinModelPortali Q8NX62.
    SMRi Q8NX62. Positions 1-107.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196620.MW1018.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB94883 ; BAB94883 ; BAB94883 .
    GeneIDi 1003130.
    KEGGi sam:MW1018.
    PATRICi 19568616. VBIStaAur44266_1067.

    Phylogenomic databases

    eggNOGi COG2329.
    HOGENOMi HOG000008026.
    KOi K07145.
    OMAi VRHQSQD.
    OrthoDBi EOG6GTZMS.

    Enzyme and pathway databases

    BioCyci SAUR196620:GJ9Z-1042-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q8NX62.

    Family and domain databases

    HAMAPi MF_01272. Heme_degrading_monooxygenase.
    InterProi IPR007138. ABM-like.
    IPR011008. Dimeric_a/b-barrel.
    IPR023953. IsdG.
    [Graphical view ]
    Pfami PF03992. ABM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54909. SSF54909. 1 hit.
    PROSITEi PS51725. ABM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MW2.
    2. "Staphylococcus aureus IsdG and IsdI, heme-degrading enzymes with structural similarity to monooxygenases."
      Wu R., Skaar E.P., Zhang R., Joachimiak G., Gornicki P., Schneewind O., Joachimiak A.
      J. Biol. Chem. 280:2840-2846(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), FUNCTION, SUBUNIT, MUTAGENESIS OF ASN-7; LYS-17; PHE-23; MET-38; TRP-67; SER-70 AND HIS-77.

    Entry informationi

    Entry nameiHDOX1_STAAW
    AccessioniPrimary (citable) accession number: Q8NX62
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    IsdG seems to carry out oxygenation of the heme without the assistance of any of the prosthetic groups or cofactors normally associated with activation of molecular oxygen.

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3