Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Heme oxygenase (staphylobilin-producing) 1

Gene

isdG

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Allows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the oxo-bilirubin chromophore staphylobilin (a mixture of the linear tetrapyrroles 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin) in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.UniRule annotation1 Publication

Catalytic activityi

Protoheme + 4 AH2 + 4 O2 = 5-oxo-delta-bilirubin + Fe2+ + CO + 4 A + 4 H2O.UniRule annotation
Protoheme + 4 AH2 + 4 O2 = 15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7IronUniRule annotation1
Sitei67Transition state stabilizerUniRule annotation1
Metal bindingi77Iron (heme axial ligand)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.14.99.48. 3352.

Names & Taxonomyi

Protein namesi
Recommended name:
Heme oxygenase (staphylobilin-producing) 1UniRule annotation (EC:1.14.99.48UniRule annotation)
Alternative name(s):
Heme-degrading monooxygenase 1UniRule annotation
Iron-regulated surface determinant 1UniRule annotation
Iron-responsive surface determinant 1UniRule annotation
Gene namesi
Name:isdG
Ordered Locus Names:MW1018
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7N → A: Loss of activity. 1 Publication1
Mutagenesisi17K → A: No effect. 1 Publication1
Mutagenesisi23F → A: No effect. 1 Publication1
Mutagenesisi38M → A: Slight loss of activity. Changes in heme binding. 1 Publication1
Mutagenesisi67W → A: Loss of activity. 1 Publication1
Mutagenesisi70S → A: No effect. 1 Publication1
Mutagenesisi77H → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002700891 – 107Heme oxygenase (staphylobilin-producing) 1Add BLAST107

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Structurei

Secondary structure

1107
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 12Combined sources10
Helixi16 – 21Combined sources6
Helixi29 – 31Combined sources3
Beta strandi35 – 45Combined sources11
Beta strandi47 – 60Combined sources14
Helixi61 – 68Combined sources8
Helixi71 – 79Combined sources9
Turni83 – 85Combined sources3
Beta strandi91 – 106Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XBWX-ray1.90A/B/C/D1-107[»]
ProteinModelPortaliQ8NX62.
SMRiQ8NX62.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NX62.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 92ABMUniRule annotationAdd BLAST90

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 29Heme bindingUniRule annotation8

Sequence similaritiesi

Belongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.UniRule annotation
Contains 1 ABM domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000008026.
KOiK07145.
OMAiIVTIMTT.

Family and domain databases

HAMAPiMF_01272. Heme_degrading_monooxygenase. 1 hit.
InterProiIPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR023953. IsdG.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NX62-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFMAENRLT LTKGTAKDII ERFYTRHGIE TLEGFDGMFV TQTLEQEDFD
60 70 80 90 100
EVKILTVWKS KQAFTDWLKS DVFKAAHKHV RSKNEDESSP IINNKVITYD

IGYSYMK
Length:107
Mass (Da):12,546
Last modified:October 1, 2002 - v1
Checksum:iDB13A134D5EC4FF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94883.1.
RefSeqiWP_000670950.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB94883; BAB94883; BAB94883.
GeneIDi28381313.
KEGGisam:MW1018.
PATRICi19568616. VBIStaAur44266_1067.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB94883.1.
RefSeqiWP_000670950.1. NC_003923.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XBWX-ray1.90A/B/C/D1-107[»]
ProteinModelPortaliQ8NX62.
SMRiQ8NX62.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB94883; BAB94883; BAB94883.
GeneIDi28381313.
KEGGisam:MW1018.
PATRICi19568616. VBIStaAur44266_1067.

Phylogenomic databases

HOGENOMiHOG000008026.
KOiK07145.
OMAiIVTIMTT.

Enzyme and pathway databases

BRENDAi1.14.99.48. 3352.

Miscellaneous databases

EvolutionaryTraceiQ8NX62.

Family and domain databases

HAMAPiMF_01272. Heme_degrading_monooxygenase. 1 hit.
InterProiIPR007138. ABM_dom.
IPR011008. Dimeric_a/b-barrel.
IPR023953. IsdG.
[Graphical view]
PfamiPF03992. ABM. 1 hit.
[Graphical view]
SUPFAMiSSF54909. SSF54909. 1 hit.
PROSITEiPS51725. ABM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHDOX1_STAAW
AccessioniPrimary (citable) accession number: Q8NX62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: October 1, 2002
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

IsdG seems to carry out oxygenation of the heme without the assistance of any of the prosthetic groups or cofactors normally associated with activation of molecular oxygen.

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.