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Q8NWS5

- DAPA_STAAW

UniProt

Q8NWS5 - DAPA_STAAW

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Protein
4-hydroxy-tetrahydrodipicolinate synthase
Gene
dapA, MW1283
Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA) By similarity.UniRule annotation

Catalytic activityi

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H2O.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei46 – 461Part of a proton relay during catalysis By similarity
Binding sitei47 – 471Pyruvate By similarity
Sitei109 – 1091Part of a proton relay during catalysis By similarity
Active sitei135 – 1351Proton donor/acceptor By similarity
Active sitei163 – 1631Schiff-base intermediate with substrate By similarity
Binding sitei206 – 2061Pyruvate; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. 4-hydroxy-tetrahydrodipicolinate synthase Source: UniProtKB-EC
  2. amine-lyase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
  2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-1307-MONOMER.
UniPathwayiUPA00034; UER00017.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate synthase (EC:4.3.3.7)
Short name:
HTPA synthase
Gene namesi
Name:dapA
Ordered Locus Names:MW1283
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000418: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2952954-hydroxy-tetrahydrodipicolinate synthaseUniRule annotation
PRO_0000103159Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196620.MW1283.

Structurei

3D structure databases

ProteinModelPortaliQ8NWS5.
SMRiQ8NWS5. Positions 2-293.

Family & Domainsi

Sequence similaritiesi

Belongs to the DapA family.

Phylogenomic databases

eggNOGiCOG0329.
HOGENOMiHOG000173604.
KOiK01714.
OMAiHPSGKSN.
OrthoDBiEOG6W7235.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00418. DapA.
InterProiIPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
TIGRFAMsiTIGR00674. dapA. 1 hit.
PROSITEiPS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NWS5-1 [UniParc]FASTAAdd to Basket

« Hide

MTHLFEGVGV ALTTPFTNNK INIEALKTHV NFLLENNAQA IIVNGTTAES    50
PTLTTDEKER ILKTVIDLVD KRVPVIAGTG TNDTEKSIQA SIQAKALGAD 100
AIMLITPYYN KTNQRGLVKH FEAIADAVKL PVVLYNVPSR TNMTIEPETV 150
EILSQHPYIV ALKDATNDFE YLEEVKKRID TNSFALYSGN DDNVVEYYQR 200
GGQGVISVIA NVIPKEFQAL YDAQQSGLDI QDQFKPIGTL LSALSVDINP 250
IPIKALTSYL GFGNYELRLP LVSLEDTDTK VLREAYDTFK AGENE 295
Length:295
Mass (Da):32,568
Last modified:October 19, 2002 - v1
Checksum:i4A41F05D949C249C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000033 Genomic DNA. Translation: BAB95148.1.
RefSeqiNP_646100.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB95148; BAB95148; BAB95148.
GeneIDi1003395.
KEGGisam:MW1283.
PATRICi19569164. VBIStaAur44266_1343.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000033 Genomic DNA. Translation: BAB95148.1 .
RefSeqi NP_646100.1. NC_003923.1.

3D structure databases

ProteinModelPortali Q8NWS5.
SMRi Q8NWS5. Positions 2-293.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196620.MW1283.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB95148 ; BAB95148 ; BAB95148 .
GeneIDi 1003395.
KEGGi sam:MW1283.
PATRICi 19569164. VBIStaAur44266_1343.

Phylogenomic databases

eggNOGi COG0329.
HOGENOMi HOG000173604.
KOi K01714.
OMAi HPSGKSN.
OrthoDBi EOG6W7235.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00017 .
BioCyci SAUR196620:GJ9Z-1307-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00418. DapA.
InterProi IPR013785. Aldolase_TIM.
IPR005263. DapA.
IPR002220. DapA-like.
IPR020625. Dihydrodipicolinate_synth_AS.
[Graphical view ]
PANTHERi PTHR12128. PTHR12128. 1 hit.
Pfami PF00701. DHDPS. 1 hit.
[Graphical view ]
PIRSFi PIRSF001365. DHDPS. 1 hit.
PRINTSi PR00146. DHPICSNTHASE.
TIGRFAMsi TIGR00674. dapA. 1 hit.
PROSITEi PS00666. DHDPS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MW2.

Entry informationi

Entry nameiDAPA_STAAW
AccessioniPrimary (citable) accession number: Q8NWS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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