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Protein

Threonine--tRNA ligase

Gene

thrS

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi336 – 3361Zinc; catalyticUniRule annotation
Metal bindingi387 – 3871Zinc; catalyticUniRule annotation
Metal bindingi517 – 5171Zinc; catalyticUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-1649-MONOMER.
BRENDAi6.1.1.3. 3352.

Names & Taxonomyi

Protein namesi
Recommended name:
Threonine--tRNA ligaseUniRule annotation (EC:6.1.1.3UniRule annotation)
Alternative name(s):
Threonyl-tRNA synthetaseUniRule annotation
Short name:
ThrRSUniRule annotation
Gene namesi
Name:thrSUniRule annotation
Ordered Locus Names:MW1626
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 645645Threonine--tRNA ligasePRO_0000101052Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

Secondary structure

1
645
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi9 – 113Combined sources
Beta strandi18 – 203Combined sources
Helixi22 – 276Combined sources
Helixi31 – 366Combined sources
Beta strandi39 – 424Combined sources
Beta strandi45 – 473Combined sources
Beta strandi58 – 625Combined sources
Helixi67 – 8822Combined sources
Beta strandi89 – 913Combined sources
Beta strandi103 – 1097Combined sources
Turni116 – 1183Combined sources
Helixi119 – 13113Combined sources
Beta strandi136 – 1405Combined sources
Turni142 – 1443Combined sources
Helixi145 – 1495Combined sources
Helixi154 – 1607Combined sources
Beta strandi164 – 1663Combined sources
Beta strandi170 – 1745Combined sources
Beta strandi177 – 1804Combined sources
Helixi189 – 1913Combined sources
Beta strandi194 – 20411Combined sources
Beta strandi209 – 2113Combined sources
Beta strandi213 – 22412Combined sources
Helixi225 – 24016Combined sources
Helixi243 – 2497Combined sources
Beta strandi253 – 2564Combined sources
Turni257 – 2593Combined sources
Beta strandi260 – 2667Combined sources
Helixi268 – 28720Combined sources
Beta strandi290 – 2923Combined sources
Beta strandi297 – 3004Combined sources
Helixi302 – 3076Combined sources
Helixi309 – 3124Combined sources
Beta strandi321 – 3233Combined sources
Turni324 – 3263Combined sources
Beta strandi327 – 3315Combined sources
Beta strandi333 – 3353Combined sources
Helixi336 – 3449Combined sources
Helixi350 – 3523Combined sources
Beta strandi355 – 36410Combined sources
Turni369 – 3713Combined sources
Turni374 – 3763Combined sources
Beta strandi379 – 39012Combined sources
Helixi392 – 3943Combined sources
Helixi395 – 41218Combined sources
Beta strandi418 – 4247Combined sources
Beta strandi430 – 4345Combined sources
Helixi437 – 45418Combined sources
Beta strandi461 – 4633Combined sources
Beta strandi471 – 4766Combined sources
Beta strandi484 – 49411Combined sources
Helixi495 – 4984Combined sources
Beta strandi506 – 5083Combined sources
Beta strandi514 – 52310Combined sources
Helixi524 – 53512Combined sources
Turni541 – 5433Combined sources
Beta strandi548 – 5547Combined sources
Helixi555 – 57016Combined sources
Turni571 – 5733Combined sources
Beta strandi576 – 5783Combined sources
Helixi585 – 59511Combined sources
Beta strandi598 – 6036Combined sources
Helixi605 – 6095Combined sources
Beta strandi612 – 6176Combined sources
Beta strandi625 – 6273Combined sources
Helixi628 – 64013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NYQX-ray3.20A/B1-645[»]
1NYRX-ray2.80A/B1-645[»]
ProteinModelPortaliQ8NW68.
SMRiQ8NW68. Positions 1-645.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8NW68.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni242 – 540299CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000003879.
OMAiFYYDFAY.
OrthoDBiEOG61KBFJ.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NW68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQINIQFPD GNKKAFDKGT TTEDIAQSIS PGLRKKAVAG KFNGQLVDLT
60 70 80 90 100
KPLETDGSIE IVTPGSEEAL EVLRHSTAHL MAHAIKRLYG NVKFGVGPVI
110 120 130 140 150
EGGFYYDFDI DQNISSDDFE QIEKTMKQIV NENMKIERKV VSRDEAKELF
160 170 180 190 200
SNDEYKLELI DAIPEDENVT LYSQGDFTDL CRGVHVPSTA KIKEFKLLST
210 220 230 240 250
AGAYWRGDSN NKMLQRIYGT AFFDKKELKA HLQMLEERKE RDHRKIGKEL
260 270 280 290 300
ELFTNSQLVG AGLPLWLPNG ATIRREIERY IVDKEVSMGY DHVYTPVLAN
310 320 330 340 350
VDLYKTSGHW DHYQEDMFPP MQLDETESMV LRPMNCPHHM MIYANKPHSY
360 370 380 390 400
RELPIRIAEL GTMHRYEASG AVSGLQRVRG MTLNDSHIFV RPDQIKEEFK
410 420 430 440 450
RVVNMIIDVY KDFGFEDYSF RLSYRDPEDK EKYFDDDDMW NKAENMLKEA
460 470 480 490 500
ADELGLSYEE AIGEAAFYGP KLDVQVKTAM GKEETLSTAQ LDFLLPERFD
510 520 530 540 550
LTYIGQDGEH HRPVVIHRGV VSTMERFVAF LTEETKGAFP TWLAPKQVQI
560 570 580 590 600
IPVNVDLHYD YARQLQDELK SQGVRVSIDD RNEKMGYKIR EAQMQKIPYQ
610 620 630 640
IVVGDKEVEN NQVNVRQYGS QDQETVEKDE FIWNLVDEIR LKKHR
Length:645
Mass (Da):74,460
Last modified:October 1, 2002 - v1
Checksum:i18FF7CFC22892FBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB95491.1.
RefSeqiWP_000435132.1. NC_003923.1.

Genome annotation databases

EnsemblBacteriaiBAB95491; BAB95491; BAB95491.
PATRICi19569866. VBIStaAur44266_1694.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA. Translation: BAB95491.1.
RefSeqiWP_000435132.1. NC_003923.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NYQX-ray3.20A/B1-645[»]
1NYRX-ray2.80A/B1-645[»]
ProteinModelPortaliQ8NW68.
SMRiQ8NW68. Positions 1-645.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB95491; BAB95491; BAB95491.
PATRICi19569866. VBIStaAur44266_1694.

Phylogenomic databases

HOGENOMiHOG000003879.
OMAiFYYDFAY.
OrthoDBiEOG61KBFJ.

Enzyme and pathway databases

BioCyciSAUR196620:GJ9Z-1649-MONOMER.
BRENDAi6.1.1.3. 3352.

Miscellaneous databases

EvolutionaryTraceiQ8NW68.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
3.40.50.800. 1 hit.
HAMAPiMF_00184. Thr_tRNA_synth.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR012675. Beta-grasp_dom.
IPR004095. TGS.
IPR012676. TGS-like.
IPR002320. Thr-tRNA-ligase_IIa.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF03129. HGTP_anticodon. 1 hit.
PF02824. TGS. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR01047. TRNASYNTHTHR.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55186. SSF55186. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00418. thrS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MW2.

Entry informationi

Entry nameiSYT_STAAW
AccessioniPrimary (citable) accession number: Q8NW68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 1, 2002
Last modified: March 16, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.