Reviewed, UniProtKB/Swiss-Prot Q8NVS9 (SSPP_STAAW)

Names and origin

Protein names

Recommended name:
    Staphopain A
    EC=3.4.22.48
Alternative name(s):
    Staphylopain A
    Staphylococcal cysteine proteinase A

Gene names

Name:	sspP
Synonyms:	scpA
Ordered Locus Names:	MW1850

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cysteine protease able to degrade elastin By similarity.
Catalytic activity	Broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate.
Enzyme regulation	Prematurely activated/folded staphopain A is inhibited by staphostatin A (scpB), which is probably required to protect staphylococcal cytoplasmic proteins from degradation by scpA By similarity.
Subunit structure	In the cytoplasm, prematurely activated/folded scpA forms a stable non-covalent complex with scpB By similarity.
Subcellular location	Secreted By similarity.
Post-translational modification	Cleavage leads to the activation of scpA probably by an auto-catalytic manner By similarity.
Miscellaneous	The catalytic maturation of scpA appears to reside outside the cascade of activation started by the metalloprotease aureolysin (aur) By similarity.
Sequence similarities	Belongs to the peptidase C47 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 214	189	By similarity		PRO_0000026557
	Chain	215 – 388	174	Staphopain A		PRO_0000026558
Sites
	Active site	238	1	By similarity
	Active site	334	1	By similarity
	Active site	355	1	By similarity
	Site	214 – 215	2	Cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NVS9-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0D502FD9F50933F7	FASTA	388	44,142
	10 20 30 40 50 60 MKRNFPKLIA LSLILSLSVT PIANAESNSN IKAKDKKHVQ VNVEDKSIPT EVRNLAQKDY 70 80 90 100 110 120 LSYVTSLDKI YNKEKASYTL GEPFKIYKFN KKSDGNYYFP VLNTEGNIDY IVTISPKVTK 130 140 150 160 170 180 YSSSSSKYTI NVSPFLSKVL NQYKDQQITI LTNSKGYYVV TQNHKAKLVL KTPRLEDKKL 190 200 210 220 230 240 KKTESIPTGN NVTQLKQKAS VTMPTSQFKS NNYTYNEQYV NKLENFKIRE TQGNNGWCAG 250 260 270 280 290 300 YTMSALLNAT YNTNKYHAEA VMRFLHPNLQ GQRFQFTGLT PREMIYFGQT QGRSPQLLNR 310 320 330 340 350 360 MTTYNEVDNL TKNNKGIAVL GSRVESRNGM HAGHAMAVVG NAKLDNGQEV IIIWNPWDNG 370 380 FMTQDAKNNV IPVSNGDHYQ WYSSIYGY

References

[1]

"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed: 12044378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000033 Genomic DNA. Translation: BAB95715.1.
RefSeq	NP_646667.1.
3D structure databases
HSSP	HSSP built from PDB template 1CV8 based on UniProtKB P81297.
SMR	Q8NVS9. Positions 216-388.
ModBase	Search...
Protein family/group databases
MEROPS	C47.001.
Genome annotation databases
GeneID	1003965.
GenomeReviews	Gene locus MW1850 in contig BA000033_GR.
KEGG	sam:MW1850.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NVS9.
OMA	Q8NVS9. YTINVSS.
Enzyme and pathway databases
BioCyc	SAUR196620:MW1850-MON.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR008750. Peptidase_C47. [Graphical view]
Pfam	PF05543. Peptidase_C47. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. False negative. PS00139. THIOL_PROTEASE_CYS. False negative. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SSPP_STAAW

Accession

Primary (citable) accession number: Q8NVS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	October 1, 2002
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)