Q8NVH3 (THID_STAAW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
EC=2.7.1.49
EC=2.7.4.7

Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name=HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name=HMP-P kinase
Short name=HMP-phosphate kinase
Short name=HMPP kinase

Gene names

Name:	thiD
Ordered Locus Names:	MW2016

Organism

Staphylococcus aureus (strain MW2) [Complete proteome] [HAMAP]

Taxonomic identifier

196620 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P By similarity.
Catalytic activity	ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine. ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3. Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.
Sequence similarities	Belongs to the ThiD family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxymethylpyrimidine kinase activity Inferred from electronic annotation. Source: EC phosphomethylpyrimidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase		PRO_0000192032
Sites
	Binding site	45	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NVH3 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 42B2E36C97F1310D	FASTA	276	30,288
	10 20 30 40 50 60 MIKPKIALTI AGTDPTGGAG VMADLKSFHS CGVYGMGVVT SIVAQNTLGV QHIHNLNHQW 70 80 90 100 110 120 VDEQLDSVFN DTLPHAIKTG MIATADTMET IRHYLMQHES IPYVIDPVML AKSGDSLMDN 130 140 150 160 170 180 DTKQNLQHTL LPLADVVTPN LPEAEEITGL TIDSEEKIMQ AGRIFINEIG SKGVIIKGGH 190 200 210 220 230 240 SNDTDIAKDY LFTNEGVQTF ENERFKTKHT HGTGCTFSAV ITAELAKGRR LFEAVHKAKK 250 260 270 FISMSIQYTP EIGRGRGPVN HFAYLKKEGL DDELSK

References

[1]

"Genome and virulence determinants of high virulence community-acquired MRSA." Baba T., Takeuchi F., Kuroda M., Yuzawa H., Aoki K., Oguchi A., Nagai Y., Iwama N., Asano K., Naimi T., Kuroda H., Cui L., Yamamoto K., Hiramatsu K. Lancet 359:1819-1827(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MW2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000033 Genomic DNA. Translation: BAB95881.1.
RefSeq	NP_646833.1. NC_003923.1.
3D structure databases
ProteinModelPortal	Q8NVH3.
ModBase	Search...
Protein-protein interaction databases
STRING	196620.MW2016.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB95881; BAB95881; BAB95881.
GeneID	1004132.
KEGG	sam:MW2016.
PATRIC	19570748. VBIStaAur44266_2092.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0351.
HOGENOM	HOG000225273.
KO	K00941.
OMA	FSAMRAY.
ProtClustDB	PRK06427.
Enzyme and pathway databases
BioCyc	SAUR196620:GJ9Z-2085-MONOMER.
UniPathway	UPA00060; UER00137. UPA00060; UER00138.
Family and domain databases
InterPro	IPR013749. HMP-P_kinase-1. IPR004399. HMP-P_kinase-2. [Graphical view]
Pfam	PF08543. Phos_pyr_kin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00097. HMP-P_kinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THID_STAAW

Accession

Primary (citable) accession number: Q8NVH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program