Q8NUD8 (DNAA_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chromosomal replication initiator protein DnaA

Gene names

Name:	dnaA
Ordered Locus Names:	Cgl0001, cg0001

Organism

Corynebacterium glutamicum (Brevibacterium flavum)

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids By similarity. HAMAP MF_00377
Subcellular location	Cytoplasm By similarity HAMAP MF_00377.
Sequence similarities	Belongs to the DnaA family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication initiation Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Chromosomal replication initiator protein DnaA HAMAP MF_00377		PRO_0000114169
Regions
	Nucleotide binding	224 – 231	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8NUD8 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 3B934D1E3F93CD2E	FASTA	524	58,508
	10 20 30 40 50 60 MSQNSSSLLE TWRQVVADLT TLSQQADSGF DPLTPTQRAY LNLTKPIAIV DGYAVLSTPN 70 80 90 100 110 120 AMAKNVIEND LGDALTRVLS LRMGRSFSLA VSVEPEQEIP ETPAQQEFKY QPDAPVISSN 130 140 150 160 170 180 KAPKQYEVGG RGEASTSDGW ERTHSAPAPE PHPAPIADPE PELATPQRIP RETPAHNPNR 190 200 210 220 230 240 EVSLNPKYTF ESFVIGPFNR FANAAAVAVA ESPAKAFNPL FISGGSGLGK THLLHAVGNY 250 260 270 280 290 300 AQELQPGLRI KYVSSEEFTN DYINSVRDDR QETFKRRYRN LDILMVDDIQ FLAGKEGTQE 310 320 330 340 350 360 EFFHTFNALH QADKQIILSS DRPPKQLTTL EDRLRTRFEG GLITDIQPPD LETRIAILMK 370 380 390 400 410 420 KAQTDGTHVD REVLELIASR FESSIRELEG ALIRVSAYSS LINQPIDKEM AIVALRDILP 430 440 450 460 470 480 EPEDMEITAP VIMEVTAEYF EISVDTLRGA GKTRAVAHAR QLAMYLCREL TDMSLPKIGD 490 500 510 520 VFGGKDHTTV MYADRKIRQE MTEKRDTYDE IQQLTQLIKS RGRN

References

[1]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB97394.1. BX927148 Genomic DNA. Translation: CAF18566.1.
RefSeq	NP_599253.1. NC_003450.3. YP_224295.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NUD8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1021144. 3342676.
GenomeReviews	Gene locus Cgl0001 in contig BA000036_GR. Gene locus cg0001 in contig BX927147_GR.
KEGG	cgb:cg0001. cgl:NCgl0001.
PATRIC	21492026. VBICorGlu203724_0001.
Phylogenomic databases
HOGENOM	HBG695666.
OMA	DEVAFLI.
PhylomeDB	Q8NUD8.
ProtClustDB	PRK00149.
Enzyme and pathway databases
BioCyc	CGLU196627:CG0001-MONOMER.
Family and domain databases
HAMAP	MF_00377. DnaA_bact. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR001957. Chromosome_initiator_DnaA. IPR020591. Chromosome_initiator_DnaA-like. IPR018312. Chromosome_initiator_DnaA_CS. IPR013159. DnaA_C. IPR013317. DnaA_Hda. IPR010921. Trp_repressor/repl_initiator. [Graphical view]
Gene3D	G3DSA:1.10.1750.10. DnaA_C. 1 hit.
KO	K02313.
Pfam	PF00308. Bac_DnaA. 1 hit. PF08299. Bac_DnaA_C. 1 hit. [Graphical view]
PRINTS	PR00051. DNAA.
SMART	SM00382. AAA. 1 hit. SM00760. Bac_DnaA_C. 1 hit. [Graphical view]
SUPFAM	SSF48295. Trp_repress_rep. 1 hit.
TIGRFAMs	TIGR00362. DnaA. 1 hit.
PROSITE	PS01008. DNAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAA_CORGL

Accession

Primary (citable) accession number: Q8NUD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	October 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program