Q8NU98 (PKN1_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase PknB
EC=2.7.11.1

Gene names

Name:	pknB
Ordered Locus Names:	Cgl0041, cg0057

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 4 PASTA domains. Contains 1 protein kinase domain.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW penicillin binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Probable serine/threonine-protein kinase PknB		PRO_0000171197
Regions
	Domain	9 – 278	270	Protein kinase
	Domain	365 – 431	67	PASTA 1
	Domain	432 – 500	69	PASTA 2
	Domain	501 – 565	65	PASTA 3
	Domain	570 – 638	69	PASTA 4
	Nucleotide binding	15 – 23	9	ATP By similarity
Sites
	Active site	136	1	Proton acceptor By similarity
	Binding site	38	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NU98 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 0F3B4980ECF73BFB	FASTA	646	68,237
	10 20 30 40 50 60 MTFVIADRYE LDAVIGSGGM SEVFAATDTL IGREVAVKML RIDLAKDPNF RERFRREAQN 70 80 90 100 110 120 SGRLSHSSIV AVFDTGEVDK DGTSVPYIVM ERVQGRNLRE VVTEDGVFTP VEAANILIPV 130 140 150 160 170 180 CEALQASHDA GIIHRDVKPA NIMITNTGGV KVMDFGIARA VNDSTSAMTQ TSAVIGTAQY 190 200 210 220 230 240 LSPEQARGKP ADARSDIYAT GCVMYELVTG KPPFEGESPF AVAYQHVQED PTPPSDFIAD 250 260 270 280 290 300 LTPTSAVNVD AVVLTAMAKH PADRYQTASE MAADLGRLSR NAVSHAARAH VETEETPEEP 310 320 330 340 350 360 ETRFSTRTST QVAPAAGVAA ASTGSGSSSR KRGSRGLTAL AIVLSLGVVG VAGAFTYDYF 370 380 390 400 410 420 ANSSSTATSA IPNVEGLPQQ EALTELQAAG FVVNIVEEAS ADVAEGLVIR ANPSVGSEIR 430 440 450 460 470 480 QGATVTITVS TGREMINIPD VSGMTLEDAA RALEDVGLIL NQNVREETSD DVESGLVIDQ 490 500 510 520 530 540 NPEAGQEVVV GSSVSLTMSS GTESIRVPNL TGMNWSQAEQ NLISMGFNPT ASYLDSSEPE 550 560 570 580 590 600 GEVLSVSSQG TELPKGSSIT VEVSNGMLIQ APDLARMSTE QAISALRAAG WTAPDQSLIV 610 620 630 640 GDPIHTAALV DQNKIGFQSP TPATLFRKDA QVQVRLFEFD LAALVQ

References

[1]

"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB97434.1. BX927148 Genomic DNA. Translation: CAF18609.1.
RefSeq	NP_599293.1. NC_003450.3. YP_224338.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NU98.
SMR	Q8NU98. Positions 5-278.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48321N.
STRING	196627.cg0057.
PTM databases
PhosSite	P0904605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB97434; BAB97434; BAB97434. CAF18609; CAF18609; cg0057.
GeneID	1021362. 3344986.
KEGG	cgb:cg0057. cgl:NCgl0040.
PATRIC	21492118. VBICorGlu203724_0042.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000037185.
KO	K08884.
OMA	TGKDPGT.
ProtClustDB	CLSK632622.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-45-MONOMER.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR005543. PASTA. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF03793. PASTA. 4 hits. PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00740. PASTA. 3 hits. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51178. PASTA. 3 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKN1_CORGL

Accession

Primary (citable) accession number: Q8NU98

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program