Q8NU97 (PKN2_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinases PknA
EC=2.7.11.1

Gene names

Name:	pknA
Synonyms:	drp72
Ordered Locus Names:	Cgl0042, cg0059

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Sequence caution	The sequence BAB88662.1 differs from that shown. Reason: Frameshift at position 348.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Serine/threonine-protein kinases PknA		PRO_0000171199
Regions
	Domain	20 – 281	262	Protein kinase
	Nucleotide binding	26 – 34	9	ATP By similarity
	Compositional bias	354 – 440	87	Thr-rich
Sites
	Active site	148	1	Proton acceptor By similarity
	Binding site	49	1	ATP By similarity
Experimental info
	Sequence conflict	119	1	R → S in BAB88662. Ref.1
	Sequence conflict	143	1	D → N in BAB88662. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8NU97 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 53C10AD97BB95595	FASTA	469	50,375
	10 20 30 40 50 60 MSQEDITGKD RLQELIGADY RLQWIIGHGG MSTVWLADDV VNDREVAIKV LRPEFSDNQE 70 80 90 100 110 120 FLNRFRNEAQ AAENIDSEHV VATYDYREVP DPAGHTFCFI VMEFVRGESL ADLLEREGRL 130 140 150 160 170 180 PEDLALDVME QAAHGLSVIH RMDMVHRDIK PGNMLITANG IVKITDFGIA KAAAAVPLTR 190 200 210 220 230 240 TGMVVGTAQY VSPEQAQGKE VTAASDIYSL GVVGYEMMAG RRPFTGDSSV SVAIAHINQA 250 260 270 280 290 300 PPQMPTSISA QTRELIGIAL RKDPGRRFPD GNEMALAVSA VRLGKRPPQP RTSAMMAQAE 310 320 330 340 350 360 APSPSESTAM LGRVARPATI TQEAAPKRGS GIGIGLFIAA LLAVIIGAVI YAGTTGILFN 370 380 390 400 410 420 DTPEETTTPE TITETYTPTV EETTSQWVPP TPPTRSTFTE PETTSHRPTT SEESTSEEPT 430 440 450 460 TEAPTSSRTV PQIPTSTPRT SASVPVETNA PADDLIDAVN GLLDVGGAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"drp72 of Corynebacterium glutamicum." Hirano S., Kimura E., Kawahara Y., Sugimoto S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB083046 Genomic DNA. Translation: BAB88662.1. Frameshift. BA000036 Genomic DNA. Translation: BAB97435.1. BX927148 Genomic DNA. Translation: CAF18610.1.
RefSeq	NP_599294.1. NC_003450.3. YP_224339.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NU97.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48320N.
STRING	196627.cg0059.
PTM databases
PhosSite	P0904601.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB97435; BAB97435; BAB97435. CAF18610; CAF18610; cg0059.
GeneID	1021413. 3343790.
KEGG	cgb:cg0059. cgl:NCgl0041.
PATRIC	21492120. VBICorGlu203724_0043.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000037187.
KO	K08884.
OMA	NEMALAV.
ProtClustDB	CLSK632623.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PKN2_CORGL

Accession

Primary (citable) accession number: Q8NU97
Secondary accession number(s): Q8RQP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	October 1, 2002
Last modified:	May 29, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program