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Protein

Endonuclease III

Gene

nth

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi212 – 2121Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi219 – 2191Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi222 – 2221Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi228 – 2281Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-HAMAP
  3. DNA binding Source: UniProtKB-HAMAP
  4. DNA N-glycosylase activity Source: UniProtKB-HAMAP
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. base-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseImported, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported, Nuclease

Keywords - Biological processi

DNA damage, DNA repairUniRule annotation

Keywords - Ligandi

4Fe-4SUniRule annotation, DNA-bindingUniRule annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciCGLU1204414-WGS:GSME-288-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotationImported
Ordered Locus Names:cg0353Imported, Cgl0293Imported
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)Imported
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi196627.cg0353.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini125 – 15127HhHUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation
Contains 1 HhH domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252208.
KOiK10773.
OMAiNNKSKHL.
OrthoDBiEOG6H4KC5.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFiPIRSF001435. Nth. 1 hit.
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NTL4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSITPQKRP RVGSHIANKG QETDIGRKRR ARRINRTLTV AYPDAHCELD
60 70 80 90 100
FTNPLELTVA TILSAQCTDV RVNQVTPALF KRYPTATDYA NADRTELEEF
110 120 130 140 150
IRPTGFYRNK ATSLIGLGEA LISLHDGQVP GTLEQLVELP GVGRKTANVV
160 170 180 190 200
LGNAFGVPGI TVDTHFGRLV RRLKLTDEED PVKVEKVMNE LIEKPEWTMF
210 220 230 240 250
SHRLIFHGRR ICHSRRAACG ACMLAADCPS FGLEGPSDPF EAQKLIKSDD
260
REHLLKMAGM
Length:260
Mass (Da):28,918
Last modified:October 1, 2002 - v1
Checksum:i823BACDBDE55190F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB97686.1.
BX927148 Genomic DNA. Translation: CAF18863.1.
RefSeqiNP_599545.1. NC_003450.3.
YP_224592.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB97686; BAB97686; BAB97686.
CAF18863; CAF18863; cg0353.
GeneIDi1021358.
3343750.
KEGGicgb:cg0353.
cgl:NCgl0288.
PATRICi21492650. VBICorGlu203724_0297.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB97686.1.
BX927148 Genomic DNA. Translation: CAF18863.1.
RefSeqiNP_599545.1. NC_003450.3.
YP_224592.1. NC_006958.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg0353.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB97686; BAB97686; BAB97686.
CAF18863; CAF18863; cg0353.
GeneIDi1021358.
3343750.
KEGGicgb:cg0353.
cgl:NCgl0288.
PATRICi21492650. VBICorGlu203724_0297.

Phylogenomic databases

HOGENOMiHOG000252208.
KOiK10773.
OMAiNNKSKHL.
OrthoDBiEOG6H4KC5.

Enzyme and pathway databases

BioCyciCGLU1204414-WGS:GSME-288-MONOMER.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFiPIRSF001435. Nth. 1 hit.
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genomic sequence of Corynebacterium glutamicum ATCC 13032."
    Nakagawa S.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025Imported.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025Imported and DSM 20300Imported.
  4. Kalinowski J.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 20300Imported.

Entry informationi

Entry nameiQ8NTL4_CORGL
AccessioniPrimary (citable) accession number: Q8NTL4
Secondary accession number(s): Q6M869
Entry historyi
Integrated into UniProtKB/TrEMBL: October 1, 2002
Last sequence update: October 1, 2002
Last modified: March 4, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.