Reviewed, UniProtKB/Swiss-Prot Q8NTB0 (MURB2_CORGL)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylenolpyruvoylglucosamine reductase 2
    EC=1.1.1.158
Alternative name(s):
    UDP-N-acetylmuramate dehydrogenase 2

Gene names

Name:	murB2
Ordered Locus Names:	Cgl0397, cg0476

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	UDP-N-acetylenolpyruvoylglucosamine reductase 2 HAMAP MF_00037		PRO_0000179204
Regions
	Domain	31 – 198	168	FAD-binding PCMH-type
Sites
	Active site	176	1	By similarity
	Active site	256	1	Proton donor By similarity
	Active site	357	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NTB0-1 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 269CDF67A484A6A4	FASTA	367	38,886
	10 20 30 40 50 60 MDSSLAQEIA AIDGVELDSE VTFADLTTLR IGGKPRSAVR CQTTEALVSA IKLLDDASLP 70 80 90 100 110 120 LLIVGGGSNL VVADGDLDVI AVIIETDDVS INLTDGLLTA DAGAVWDDVV HLSVDAGLGG 130 140 150 160 170 180 IECLSGIPGS AGATPVQNVG AYGTEVSDVL TRVQLLDRTT HQVSWVDASE LDLSYRYSNL 190 200 210 220 230 240 KFTNRAVVLA IELQLLTDGL SAPLRFGELG RRLAISEAEP HPRRPVRMVR DAVLELRRAK 250 260 270 280 290 300 GMVVEHTDHD TWSAGSFFTN PIVDPALADA VFEKVGEPTM PRFPAGDGKE KLSAAWLIER 310 320 330 340 350 360 AGFKKGHPGA GAKASLSTKH TLALTNRGDA RASDLVALAK EIRDGVLETF GVTLVPEPVW IGISIDD

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000036 Genomic DNA. Translation: BAB97790.1. BX927149 Genomic DNA. Translation: CAF19112.1. Different initiation.
RefSeq	NP_599645.1. YP_224698.1.
3D structure databases
HSSP	HSSP built from PDB template 2MBR based on UniProtKB P08373.
ModBase	Search...
Genome annotation databases
GeneID	1021429. 3344837.
GenomeReviews	Gene locus Cgl0397 in contig BA000036_GR. Gene locus cg0476 in contig BX927147_GR.
KEGG	cgb:cg0476. cgl:NCgl0386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8NTB0.
OMA	FAGVEWD.
Enzyme and pathway databases
BioCyc	CGLU196627-1:CG0476-MON.
BRENDA	1.1.1.158. 812.
Family and domain databases
HAMAP	MF_00037. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB2_CORGL

Accession

Primary (citable) accession number: Q8NTB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	October 1, 2002
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)