Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.1 Publication

Kineticsi

  1. KM=0.21 mM for UDP-GlcNAc1 Publication
  2. KM=0.24 mM for 1D-inositol 3-phosphate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei91D-inositol 3-phosphate1
    Binding sitei23UDP-GlcNAc; via amide nitrogen1
    Binding sitei781D-inositol 3-phosphate1
    Binding sitei1101D-inositol 3-phosphate1
    Binding sitei1341D-inositol 3-phosphate1
    Binding sitei1541D-inositol 3-phosphate1
    Binding sitei231UDP-GlcNAc1
    Binding sitei236UDP-GlcNAc1
    Binding sitei294UDP-GlcNAc; via amide nitrogen and carbonyl oxygen1
    Metal bindingi303Magnesium; via carbonyl oxygen1
    Metal bindingi304Magnesium; via carbonyl oxygen1
    Metal bindingi306Magnesium; via carbonyl oxygen1
    Binding sitei316UDP-GlcNAc1
    Binding sitei324UDP-GlcNAc1
    Metal bindingi330Magnesium1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciCORYNE:G18NG-9958-MONOMER.
    MetaCyc:G18HP-6702-MONOMER.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250)
    Alternative name(s):
    N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase
    Short name:
    GlcNAc-Ins-P N-acetylglucosaminyltransferase
    Gene namesi
    Name:mshA
    Ordered Locus Names:Cgl0401, cg0481
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
    Proteomesi
    • UP000000582 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003998231 – 418D-inositol 3-phosphate glycosyltransferaseAdd BLAST418

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi196627.NCgl0389.

    Structurei

    Secondary structure

    1418
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 6Combined sources5
    Helixi24 – 37Combined sources14
    Beta strandi41 – 47Combined sources7
    Helixi51 – 53Combined sources3
    Beta strandi55 – 60Combined sources6
    Beta strandi63 – 68Combined sources6
    Beta strandi73 – 75Combined sources3
    Helixi78 – 84Combined sources7
    Helixi85 – 99Combined sources15
    Beta strandi104 – 109Combined sources6
    Helixi110 – 124Combined sources15
    Beta strandi128 – 131Combined sources4
    Helixi136 – 139Combined sources4
    Helixi149 – 164Combined sources16
    Beta strandi166 – 172Combined sources7
    Helixi173 – 183Combined sources11
    Helixi187 – 189Combined sources3
    Beta strandi190 – 192Combined sources3
    Turni199 – 201Combined sources3
    Helixi211 – 216Combined sources6
    Beta strandi221 – 230Combined sources10
    Helixi234 – 236Combined sources3
    Helixi238 – 251Combined sources14
    Beta strandi256 – 262Combined sources7
    Helixi274 – 281Combined sources8
    Turni285 – 287Combined sources3
    Beta strandi288 – 291Combined sources4
    Helixi296 – 305Combined sources10
    Beta strandi307 – 311Combined sources5
    Helixi320 – 327Combined sources8
    Beta strandi332 – 336Combined sources5
    Helixi340 – 343Combined sources4
    Turni346 – 348Combined sources3
    Beta strandi349 – 355Combined sources7
    Helixi358 – 370Combined sources13
    Helixi372 – 407Combined sources36

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3C48X-ray2.10A/B1-418[»]
    3C4QX-ray2.80A/B1-418[»]
    3C4VX-ray2.60A/B1-418[»]
    ProteinModelPortaliQ8NTA6.
    SMRiQ8NTA6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ8NTA6.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni15 – 16UDP-GlcNAc binding2
    Regioni20 – 251D-inositol 3-phosphate binding6

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4107R5M. Bacteria.
    COG0438. LUCA.
    HOGENOMiHOG000077288.
    KOiK15521.
    OMAiHTMAKVK.

    Family and domain databases

    HAMAPiMF_01695. MshA. 1 hit.
    InterProiIPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4-like_N.
    IPR017814. Mycothiol_biosynthesis_MshA.
    [Graphical view]
    PfamiPF13439. Glyco_transf_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8NTA6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRVAMISMHT SPLQQPGTGD SGGMNVYILS TATELAKQGI EVDIYTRATR
    60 70 80 90 100
    PSQGEIVRVA ENLRVINIAA GPYEGLSKEE LPTQLAAFTG GMLSFTRREK
    110 120 130 140 150
    VTYDLIHSHY WLSGQVGWLL RDLWRIPLIH TAHTLAAVKN SYRDDSDTPE
    160 170 180 190 200
    SEARRICEQQ LVDNADVLAV NTQEEMQDLM HHYDADPDRI SVVSPGADVE
    210 220 230 240 250
    LYSPGNDRAT ERSRRELGIP LHTKVVAFVG RLQPFKGPQV LIKAVAALFD
    260 270 280 290 300
    RDPDRNLRVI ICGGPSGPNA TPDTYRHMAE ELGVEKRIRF LDPRPPSELV
    310 320 330 340 350
    AVYRAADIVA VPSFNESFGL VAMEAQASGT PVIAARVGGL PIAVAEGETG
    360 370 380 390 400
    LLVDGHSPHA WADALATLLD DDETRIRMGE DAVEHARTFS WAATAAQLSS
    410
    LYNDAIANEN VDGETHHG
    Length:418
    Mass (Da):45,669
    Last modified:October 1, 2002 - v1
    Checksum:i3865B32C25DB6C07
    GO

    Sequence cautioni

    The sequence CAF19117 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000036 Genomic DNA. Translation: BAB97794.1.
    BX927149 Genomic DNA. Translation: CAF19117.1. Different initiation.
    RefSeqiNP_599648.1. NC_003450.3.
    WP_011013628.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB97794; BAB97794; BAB97794.
    CAF19117; CAF19117; cg0481.
    GeneIDi1021208.
    KEGGicgb:cg0481.
    cgl:NCgl0389.
    PATRICi21492860. VBICorGlu203724_0401.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000036 Genomic DNA. Translation: BAB97794.1.
    BX927149 Genomic DNA. Translation: CAF19117.1. Different initiation.
    RefSeqiNP_599648.1. NC_003450.3.
    WP_011013628.1. NC_006958.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3C48X-ray2.10A/B1-418[»]
    3C4QX-ray2.80A/B1-418[»]
    3C4VX-ray2.60A/B1-418[»]
    ProteinModelPortaliQ8NTA6.
    SMRiQ8NTA6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi196627.NCgl0389.

    Protein family/group databases

    CAZyiGT4. Glycosyltransferase Family 4.

    Protocols and materials databases

    DNASUi1021208.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiBAB97794; BAB97794; BAB97794.
    CAF19117; CAF19117; cg0481.
    GeneIDi1021208.
    KEGGicgb:cg0481.
    cgl:NCgl0389.
    PATRICi21492860. VBICorGlu203724_0401.

    Phylogenomic databases

    eggNOGiENOG4107R5M. Bacteria.
    COG0438. LUCA.
    HOGENOMiHOG000077288.
    KOiK15521.
    OMAiHTMAKVK.

    Enzyme and pathway databases

    BioCyciCORYNE:G18NG-9958-MONOMER.
    MetaCyc:G18HP-6702-MONOMER.

    Miscellaneous databases

    EvolutionaryTraceiQ8NTA6.

    Family and domain databases

    HAMAPiMF_01695. MshA. 1 hit.
    InterProiIPR001296. Glyco_trans_1.
    IPR028098. Glyco_trans_4-like_N.
    IPR017814. Mycothiol_biosynthesis_MshA.
    [Graphical view]
    PfamiPF13439. Glyco_transf_4. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMSHA_CORGL
    AccessioniPrimary (citable) accession number: Q8NTA6
    Secondary accession number(s): Q6M7W1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: October 1, 2002
    Last modified: November 2, 2016
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.