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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651PotassiumBy similarity
Metal bindingi123 – 1231PotassiumBy similarity
Binding sitei156 – 1561NAD(P)HX (for epimerase activity)By similarity
Metal bindingi159 – 1591PotassiumBy similarity
Binding sitei365 – 3651NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity
Binding sitei490 – 4901NAD(P)HX (for dehydratase activity)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi461 – 4655ADPBy similarity
Nucleotide bindingi480 – 48910ADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Ligandi

ATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:Cgl0595, cg0688
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Bifunctional NAD(P)H-hydrate repair enzyme NnrPRO_0000416415Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196627.cg0688.

Structurei

3D structure databases

ProteinModelPortaliQ8NSS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 231222YjeF N-terminalAdd
BLAST
Domaini277 – 568292YjeF C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 269269NAD(P)H-hydrate epimeraseBy similarityAdd
BLAST
Regioni64 – 685NAD(P)HX (for epimerase activity)By similarity
Regioni127 – 1337NAD(P)HX (for epimerase activity)By similarity
Regioni277 – 574298ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd
BLAST
Regioni418 – 4247NAD(P)HX (for dehydratase activity)By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated
Contains 1 YjeF C-terminal domain.Curated
Contains 1 YjeF N-terminal domain.Curated

Phylogenomic databases

eggNOGiCOG0062. LUCA.
COG0063. LUCA.
HOGENOMiHOG000228405.
OMAiWPTIDSK.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.10260. 1 hit.
HAMAPiMF_01965. NADHX_dehydratase. 1 hit.
MF_01966. NADHX_epimerase. 1 hit.
InterProiIPR000631. CARKD.
IPR029056. Ribokinase-like.
IPR004443. YjeF_N_dom.
[Graphical view]
PfamiPF01256. Carb_kinase. 1 hit.
PF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
SSF64153. SSF64153. 1 hit.
PROSITEiPS51383. YJEF_C_3. 1 hit.
PS51385. YJEF_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NSS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPVFSVDQI RRAENTLFEL QADPDELMIS AASAVADVAL AMVDGPAPAV
60 70 80 90 100
SSEESILLLV GPGGNGGDAL YAGAFLAEEG HHVDALLLGN GKVHQSALAY
110 120 130 140 150
YESLGGQIIS DFPPHYLYRL VIDGLFGIGG RGGLTPELAS LVESFSASGI
160 170 180 190 200
PILAIDVPSG VHADSGELPP GVMVTVEGFD NDAPMARQKI PAHIDADVTI
210 220 230 240 250
TFGGLRRAHA VSPACGEVLC ADINIAGGGG KSLSAELSQV QAEDATPQMF
260 270 280 290 300
ASKAYQRKDS LFERANLKAT APHIHRIGQH FTVLNMEPGP DHDKYSGGIV
310 320 330 340 350
GIVAGSGTYP GAAVLSVKAA VRATSAMVRY VGPALNFVIQ SLPEVVATQS
360 370 380 390 400
LATAGRVQAW VHGPGRGLEA EQSAELAELL SRPEPVLIDA DSLSLLQLSA
410 420 430 440 450
ELRQALRERK APTVLTPHKG EFERIAAELR SEGVEIPQAD KDPIGAAQAL
460 470 480 490 500
AKEFDCCVLL KGKYTVIAAH DFVHAINAGH SWLATPGSGD VLSGLVGAHL
510 520 530 540 550
AQSYAELNRL PEFFPDVTLS DSAIYTQIAP AATIHAVAAG LAARTEFGFA
560 570
PTSASLIADA IPAATAKVDL KRIV
Length:574
Mass (Da):59,796
Last modified:October 1, 2002 - v1
Checksum:i23A7F967ED25B1D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB97988.1.
BX927149 Genomic DNA. Translation: CAF19300.1.
RefSeqiNP_599831.1. NC_003450.3.
WP_011013753.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB97988; BAB97988; BAB97988.
CAF19300; CAF19300; cg0688.
GeneIDi1018599.
KEGGicgb:cg0688.
cgl:NCgl0570.
PATRICi21493240. VBICorGlu203724_0586.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB97988.1.
BX927149 Genomic DNA. Translation: CAF19300.1.
RefSeqiNP_599831.1. NC_003450.3.
WP_011013753.1. NC_006958.1.

3D structure databases

ProteinModelPortaliQ8NSS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg0688.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB97988; BAB97988; BAB97988.
CAF19300; CAF19300; cg0688.
GeneIDi1018599.
KEGGicgb:cg0688.
cgl:NCgl0570.
PATRICi21493240. VBICorGlu203724_0586.

Phylogenomic databases

eggNOGiCOG0062. LUCA.
COG0063. LUCA.
HOGENOMiHOG000228405.
OMAiWPTIDSK.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.10260. 1 hit.
HAMAPiMF_01965. NADHX_dehydratase. 1 hit.
MF_01966. NADHX_epimerase. 1 hit.
InterProiIPR000631. CARKD.
IPR029056. Ribokinase-like.
IPR004443. YjeF_N_dom.
[Graphical view]
PfamiPF01256. Carb_kinase. 1 hit.
PF03853. YjeF_N. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
SSF64153. SSF64153. 1 hit.
PROSITEiPS51383. YJEF_C_3. 1 hit.
PS51385. YJEF_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNR_CORGL
AccessioniPrimary (citable) accession number: Q8NSS3
Secondary accession number(s): Q6M7G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: October 1, 2002
Last modified: September 7, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.