Q8NSJ4 (SYW_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan--tRNA ligase
EC=6.1.1.2

Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS

Gene names

Name:	trpS
Ordered Locus Names:	Cgl0677, cg0779

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tryptophan-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Tryptophan--tRNA ligase HAMAP-Rule MF_00140		PRO_0000136626
Regions
	Motif	22 – 31	10	"HIGH" region HAMAP-Rule MF_00140
	Motif	207 – 211	5	"KMSKS" region HAMAP-Rule MF_00140
Sites
	Binding site	210	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8NSJ4 [UniParc]. Last modified October 1, 2002. Version 1. Checksum: 13A34953B0583455	FASTA	345	37,827
	10 20 30 40 50 60 MTTQDKDLTA QTASRVLSGI QPTADSYHLG NYLGAVKQWI DLQDSYDAFY FIPDLHAITV 70 80 90 100 110 120 DQEPEELRNR TISGAAQLLA LGIDPERSTL FVQSHVPAHA ELSWVLTCLT GFGEASRMTQ 130 140 150 160 170 180 FKDKSSKRGA DRTSAGLFTY PMLMAADILL YRPHLVPVGE DQRQHLELTR TLAERFNNRF 190 200 210 220 230 240 GKAFEVPEGF IPQGASKIYD LQNPTAKMSK SGDNPKGIIN LLDDPKVSTK RIKSAVTDND 250 260 270 280 290 300 GVIAYDPENK PGVSNLLVIQ SALTGTSIDS LVDGYQGAGY GALKGDTADA LEAFTTPLKA 310 320 330 340 KYDEYMNDRG ELERVLAIGA ERATEVANET LADVYDKIGF LASRR

References

[1]

"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB98070.1. BX927149 Genomic DNA. Translation: CAF19382.1.
RefSeq	NP_599909.1. NC_003450.3. YP_224968.1. NC_006958.1.
3D structure databases
ProteinModelPortal	Q8NSJ4.
SMR	Q8NSJ4. Positions 14-341.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg0779.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB98070; BAB98070; BAB98070. CAF19382; CAF19382; cg0779.
GeneID	1018676. 3344474.
KEGG	cgb:cg0779. cgl:NCgl0647.
PATRIC	21493396. VBICorGlu203724_0663.
Phylogenomic databases
eggNOG	COG0180.
HOGENOM	HOG000059940.
KO	K01867.
OMA	HDCLYCV.
ProtClustDB	PRK00927.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-670-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00140_B. Trp_tRNA_synth_B.
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002306. Trp-tRNA-ligase. IPR024109. Trp-tRNA-ligase_bac-type. [Graphical view]
PANTHER	PTHR10055. PTHR10055. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01039. TRNASYNTHTRP.
TIGRFAMs	TIGR00233. trpS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYW_CORGL

Accession

Primary (citable) accession number: Q8NSJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program