ID   SAHH_CORGL              Reviewed;         474 AA.
AC   Q8NSC4;
DT   25-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   16-JUN-2009, entry version 44.
DE   RecName: Full=Adenosylhomocysteinase;
DE            EC=3.3.1.1;
DE   AltName: Full=S-adenosyl-L-homocysteine hydrolase;
DE            Short=AdoHcyase;
GN   Name=ahcY; Synonyms=sahH; OrderedLocusNames=Cgl0752, cg0860;
OS   Corynebacterium glutamicum (Brevibacterium flavum).
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Corynebacteriaceae; Corynebacterium.
OX   NCBI_TaxID=1718;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
RA   Nakagawa S.;
RT   "Complete genomic sequence of Corynebacterium glutamicum ATCC 13032.";
RL   Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025;
RX   MEDLINE=22830012; PubMed=12948626; DOI=10.1016/S0168-1656(03)00154-8;
RA   Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M.,
RA   Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L.,
RA   Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B.,
RA   McHardy A.C., Meyer F., Moeckel B., Pfefferle W., Puehler A.,
RA   Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I.,
RA   Tauch A.;
RT   "The complete Corynebacterium glutamicum ATCC 13032 genome sequence
RT   and its impact on the production of L-aspartate-derived amino acids
RT   and vitamins.";
RL   J. Biotechnol. 104:5-25(2003).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-homocysteine + H(2)O = L-
CC       homocysteine + adenosine.
CC   -!- COFACTOR: Binds 1 NAD per subunit (By similarity).
CC   -!- PATHWAY: Amino-acid biosynthesis; homocysteine biosynthesis; L-
CC       homocysteine from S-adenosyl-L-homocysteine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the adenosylhomocysteinase family.
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DR   EMBL; BA000036; BAB98145.1; -; Genomic_DNA.
DR   EMBL; BX927150; CAF19456.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_599981.1; -.
DR   RefSeq; YP_225042.1; -.
DR   HSSP; P10760; 1B3R.
DR   GeneID; 1018748; -.
DR   GeneID; 3343536; -.
DR   GenomeReviews; BA000036_GR; Cgl0752.
DR   GenomeReviews; BX927147_GR; cg0860.
DR   KEGG; cgb:cg0860; -.
DR   KEGG; cgl:NCgl0719; -.
DR   HOGENOM; Q8NSC4; -.
DR   OMA; Q8NSC4; HMRAMKD.
DR   BioCyc; CGLU196627-1:CG0860-MON; -.
DR   BRENDA; 3.3.1.1; 812.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004013; F:adenosylhomocysteinase activity; IEA:HAMAP.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:HAMAP.
DR   HAMAP; MF_00563; -; 1.
DR   InterPro; IPR000043; Ad_hcy_hydrolase.
DR   InterPro; IPR015878; Ado_hCys_hydrolase_NAD-bd.
DR   Gene3D; G3DSA:3.40.50.1480; Ad_hcy_hydrolase; 1.
DR   PANTHER; PTHR23420; Ad_hcy_hydrolase; 1.
DR   Pfam; PF05221; AdoHcyase; 1.
DR   Pfam; PF00670; AdoHcyase_NAD; 1.
DR   PIRSF; PIRSF001109; Ad_hcy_hydrolase; 1.
DR   TIGRFAMs; TIGR00936; ahcY; 1.
DR   PROSITE; PS00738; ADOHCYASE_1; 1.
DR   PROSITE; PS00739; ADOHCYASE_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Hydrolase; NAD; One-carbon metabolism.
FT   CHAIN         1    474       Adenosylhomocysteinase.
FT                                /FTId=PRO_0000116960.
FT   REGION      224    392       NAD binding (By similarity).
FT   BINDING      53     53       Substrate (By similarity).
FT   BINDING     135    135       Substrate (By similarity).
FT   BINDING     197    197       Substrate (By similarity).
FT   BINDING     227    227       Substrate (By similarity).
FT   BINDING     231    231       Substrate (By similarity).
SQ   SEQUENCE   474 AA;  51996 MW;  74673F92624C4F9D CRC64;
     MDFKVADLSL AEAGRHQIRL AEYEMPGLMQ LRKEFADEQP LKGARIAGSI HMTVQTAVLI
     ETLTALGAEV RWASCNIFST QDEAAAAIVV GSGTVEEPAG VPVFAWKGES LEEYWWCINQ
     IFSWGDELPN MILDDGGDAT MAVIRGREYE QAGLVPPAEA NDSDEYIAFL GMLREVLAAE
     PGKWGKIAEA VKGVTEETTT GVHRLYHFAE EGVLPFPAMN VNDAVTKSKF DNKYGTRHSL
     IDGINRATDM LMGGKNVLVC GYGDVGKGCA EAFDGQGARV KVTEADPINA LQALMDGYSV
     VTVDEAIEDA DIVITATGNK DIISFEQMLK MKDHALLGNI GHFDNEIDMH SLLHRDDVTR
     TTIKPQVDEF TFSTGRSIIV LSEGRLLNLG NATGHPSFVM SNSFADQTIA QIELFQNEGQ
     YENEVYRLPK VLDEKVARIH VEALGGQLTE LTKEQAEYIG VDVAGPFKPE HYRY
//