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Protein

Thymidylate synthase

Gene

thyA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Cgl0844, cg0966
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001009 Componenti: Chromosome UP000000582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Thymidylate synthasePRO_0000140952Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg0966.

Structurei

Secondary structure

1
266
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1712Combined sources
Beta strandi19 – 213Combined sources
Beta strandi29 – 4012Combined sources
Helixi41 – 433Combined sources
Beta strandi49 – 513Combined sources
Helixi55 – 6713Combined sources
Beta strandi70 – 723Combined sources
Helixi73 – 775Combined sources
Turni82 – 865Combined sources
Helixi97 – 1037Combined sources
Helixi114 – 12411Combined sources
Beta strandi132 – 1343Combined sources
Turni138 – 1403Combined sources
Helixi141 – 1433Combined sources
Beta strandi144 – 1463Combined sources
Beta strandi149 – 15810Combined sources
Beta strandi161 – 17212Combined sources
Turni173 – 1764Combined sources
Helixi177 – 19418Combined sources
Beta strandi198 – 21215Combined sources
Helixi213 – 2153Combined sources
Helixi216 – 2238Combined sources
Beta strandi232 – 2354Combined sources
Helixi246 – 2483Combined sources
Beta strandi249 – 2535Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H0RX-ray2.30A/B1-266[»]
4H0UX-ray2.75A/B/C/D1-266[»]
ProteinModelPortaliQ8NS38.
SMRiQ8NS38. Positions 7-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NS38-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVPTPYEDL LRKIAEEGSH KDDRTGTGTT SLFGQQIRFD LNEGFPLLTT
60 70 80 90 100
KKVHFHSVVG ELLWFLQGDS NVKWLQDNNI RIWNEWADED GELGPVYGVQ
110 120 130 140 150
WRSWPTPDGR HIDQISGALE TLRNNPDSRR NIVSAWNVSE LENMALPPCH
160 170 180 190 200
LLFQLYVADG KLSCQLYQRS ADMFLGVPFN IASYALLTHM FAQQAGLEVG
210 220 230 240 250
EFIWTGGDCH IYDNHKEQVA EQLSREARPY PTLELNKAAS MFEYSFDDIT
260
VSGYDPHPLI RGKVAV
Length:266
Mass (Da):30,230
Last modified:October 1, 2002 - v1
Checksum:iE63BA3445460DF09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98237.1.
BX927150 Genomic DNA. Translation: CAF19550.1.
RefSeqiNP_600073.1. NC_003450.3.
WP_003862391.1. NC_006958.1.
YP_225136.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98237; BAB98237; BAB98237.
CAF19550; CAF19550; cg0966.
GeneIDi1018839.
23503601.
KEGGicgb:cg0966.
cgl:NCgl0810.
PATRICi21493738. VBICorGlu203724_0828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000036 Genomic DNA. Translation: BAB98237.1.
BX927150 Genomic DNA. Translation: CAF19550.1.
RefSeqiNP_600073.1. NC_003450.3.
WP_003862391.1. NC_006958.1.
YP_225136.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H0RX-ray2.30A/B1-266[»]
4H0UX-ray2.75A/B/C/D1-266[»]
ProteinModelPortaliQ8NS38.
SMRiQ8NS38. Positions 7-266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196627.cg0966.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB98237; BAB98237; BAB98237.
CAF19550; CAF19550; cg0966.
GeneIDi1018839.
23503601.
KEGGicgb:cg0966.
cgl:NCgl0810.
PATRICi21493738. VBICorGlu203724_0828.

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiTYSY_CORGL
AccessioniPrimary (citable) accession number: Q8NS38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: May 27, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.