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Q8NS38

- TYSY_CORGL

UniProt

Q8NS38 - TYSY_CORGL

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Protein

Thymidylate synthase

Gene

thyA

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491UniRule annotation

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dTMP biosynthetic process Source: UniProtKB-HAMAP
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:Cgl0844, cg0966
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Thymidylate synthasePRO_0000140952Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg0966.

Structurei

Secondary structure

1
266
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 1712
Beta strandi19 – 213
Beta strandi29 – 4012
Helixi41 – 433
Beta strandi49 – 513
Helixi55 – 6713
Beta strandi70 – 723
Helixi73 – 775
Turni82 – 865
Helixi97 – 1037
Helixi114 – 12411
Beta strandi132 – 1343
Turni138 – 1403
Helixi141 – 1433
Beta strandi144 – 1463
Beta strandi149 – 15810
Beta strandi161 – 17212
Turni173 – 1764
Helixi177 – 19418
Beta strandi198 – 21215
Helixi213 – 2153
Helixi216 – 2238
Beta strandi232 – 2354
Helixi246 – 2483
Beta strandi249 – 2535

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H0RX-ray2.30A/B1-266[»]
4H0UX-ray2.75A/B/C/D1-266[»]
ProteinModelPortaliQ8NS38.
SMRiQ8NS38. Positions 7-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257899.
KOiK00560.
OMAiFGRQIRY.
OrthoDBiEOG6K6V53.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8NS38-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVPTPYEDL LRKIAEEGSH KDDRTGTGTT SLFGQQIRFD LNEGFPLLTT
60 70 80 90 100
KKVHFHSVVG ELLWFLQGDS NVKWLQDNNI RIWNEWADED GELGPVYGVQ
110 120 130 140 150
WRSWPTPDGR HIDQISGALE TLRNNPDSRR NIVSAWNVSE LENMALPPCH
160 170 180 190 200
LLFQLYVADG KLSCQLYQRS ADMFLGVPFN IASYALLTHM FAQQAGLEVG
210 220 230 240 250
EFIWTGGDCH IYDNHKEQVA EQLSREARPY PTLELNKAAS MFEYSFDDIT
260
VSGYDPHPLI RGKVAV
Length:266
Mass (Da):30,230
Last modified:October 1, 2002 - v1
Checksum:iE63BA3445460DF09
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB98237.1.
BX927150 Genomic DNA. Translation: CAF19550.1.
RefSeqiNP_600073.1. NC_003450.3.
YP_225136.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB98237; BAB98237; BAB98237.
CAF19550; CAF19550; cg0966.
GeneIDi1018839.
3345293.
KEGGicgb:cg0966.
cgl:NCgl0810.
PATRICi21493738. VBICorGlu203724_0828.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000036 Genomic DNA. Translation: BAB98237.1 .
BX927150 Genomic DNA. Translation: CAF19550.1 .
RefSeqi NP_600073.1. NC_003450.3.
YP_225136.1. NC_006958.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4H0R X-ray 2.30 A/B 1-266 [» ]
4H0U X-ray 2.75 A/B/C/D 1-266 [» ]
ProteinModelPortali Q8NS38.
SMRi Q8NS38. Positions 7-266.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg0966.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB98237 ; BAB98237 ; BAB98237 .
CAF19550 ; CAF19550 ; cg0966 .
GeneIDi 1018839.
3345293.
KEGGi cgb:cg0966.
cgl:NCgl0810.
PATRICi 21493738. VBICorGlu203724_0828.

Phylogenomic databases

eggNOGi COG0207.
HOGENOMi HOG000257899.
KOi K00560.
OMAi FGRQIRY.
OrthoDBi EOG6K6V53.

Enzyme and pathway databases

UniPathwayi UPA00575 .

Family and domain databases

Gene3Di 3.30.572.10. 1 hit.
HAMAPi MF_00008. Thymidy_synth_bact.
InterProi IPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view ]
Pfami PF00303. Thymidylat_synt. 1 hit.
[Graphical view ]
PRINTSi PR00108. THYMDSNTHASE.
SUPFAMi SSF55831. SSF55831. 1 hit.
TIGRFAMsi TIGR03284. thym_sym. 2 hits.
PROSITEi PS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiTYSY_CORGL
AccessioniPrimary (citable) accession number: Q8NS38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: October 1, 2002
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3