Probable dolichyl-phosphate-mannose--protein mannosyltransferase - Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable dolichyl-phosphate-mannose--protein mannosyltransferase
EC=2.4.1.109

Gene names

Name:	pmt
Ordered Locus Names:	Cgl0890, cg1014

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium ›

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins By similarity.
Catalytic activity	Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Disruption phenotype	Loss of protein glycosylation. Ref.3
Sequence similarities	Belongs to the glycosyltransferase 39 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein O-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	dolichyl-phosphate-mannose-protein mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 520

520

Probable dolichyl-phosphate-mannose--protein mannosyltransferase

PRO_0000421157

Regions

Transmembrane

38 – 58

21

Helical; Potential

Transmembrane

119 – 139

21

Helical; Potential

Transmembrane

145 – 165

21

Helical; Potential

Transmembrane

168 – 188

21

Helical; Potential

Transmembrane

237 – 257

21

Helical; Potential

Transmembrane

274 – 294

21

Helical; Potential

Transmembrane

388 – 408

21

Helical; Potential

Transmembrane

418 – 438

21

Helical; Potential

Transmembrane

441 – 461

21

Helical; Potential

Transmembrane

485 – 505

21

Helical; Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q8NRZ6 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: 9F6C21ED4B20788D
Show »

FASTA

520

58,734

        10         20         30         40         50         60 
MSQALPVRDQ GRDQGIFAGT LPPAPPKFKW TRLDTYTWAI IAVFALVTRF TGLSSATASG 

        70         80         90        100        110        120 
TPVFDEKHYV PQAWDMVRSW INPITGGIES NPGYGLVVHP PLAKQLEALG EWVFGYTPLG 

       130        140        150        160        170        180 
WRIMVAIFGT LTIFAIMAIA RRLSGSTMVT FIAGILALAD GVLLVSSRFG MLDIFLVFFI 

       190        200        210        220        230        240 
TAAAWALIRD HQQMHQRLND LLLTNGQITK DFGPRFGFRW WRFTTGVFLG LALSVKWSGL 

       250        260        270        280        290        300 
YYIAFFGLTS VFLDLWLRKR YGVRRYVTGT LKNDVIPALG SLVIIPALLY IWSWRAWFAS 

       310        320        330        340        350        360 
ETSVYRHAKT DGTITEDSIL QLFPESIAGW IHYHISVLEF HGSLTTSSGH SHPWDSKPWA 

       370        380        390        400        410        420 
WLVSGRPILY FSSTDISCDV GGTCRRMIYL FGTPAIWWLT VPVILWALWS FFARRSRGYV 

       430        440        450        460        470        480 
VPLVAFAAGF LPWLAAYDRQ MYFFYATALV PFTIIMLALA CGELWGRGKM TPTGLTRGSM 

       490        500        510        520 
AVVTYISLVV MMFLAFSPLF YGFVIPDYVY ESLMWFPSWR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[2]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The Corynebacterium glutamicum gene pmt encoding a glycosyltransferase related to eukaryotic protein-O-mannosyltransferases is essential for glycosylation of the resuscitation promoting factor (Rpf2) and other secreted proteins." Mahne M., Tauch A., Puhler A., Kalinowski J. FEMS Microbiol. Lett. 259:226-233(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB98283.1. BX927150 Genomic DNA. Translation: CAF19596.1.
RefSeq	NP_600117.1. NC_003450.3. YP_225182.1. NC_006958.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg1014.
Protocols and materials databases
DNASU	1018883.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB98283; BAB98283; BAB98283. CAF19596; CAF19596; cg1014.
GeneID	1018883. 3343200.
KEGG	cgb:cg1014. cgl:NCgl0854.
PATRIC	21493828. VBICorGlu203724_0873.
Phylogenomic databases
HOGENOM	HOG000097888.
OMA	KHYVPQA.
ProtClustDB	CLSK2502769.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-884-MONOMER.
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR003342. Glyco_trans_39. [Graphical view]
Pfam	PF02366. PMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMT_CORGL

Accession

Primary (citable) accession number: Q8NRZ6
Secondary accession number(s): Q6M6Q4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2013
Last sequence update:	October 1, 2002
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents